Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Isolation of Bacillus amyloliquefaciens MJ-3 and Its Effect on the Early Growth Promotion of Red Pepper Plug Seedlings in Compost

고추 플러그묘 초기 생육을 촉진시키는 Bacillus amyloliquefaciens MJ-3의 분리 및 상토내 처리 효과

  • Published : 2003.10.01
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Abstract

The effect of useful rhizobacterium added in bed soil on the early growth promotion of red pepper plug seedlings was investigated. Total 540 colonies of rhizobacteria from 385 samples of eggplant family roots were isolated. Among these, 5 isolates were selected for antifungal activity against pathogenic fungi such as Alternaria solani, Botrytis cinerea, Fusarium oxysporium, Phytophthora capsici, and Sclerotia sclerotiorum. Of all the isolates, MJ-3 having the most pronounced growth-promoting ability for red pepper was finally selected and identified as Bacillus amyloliquefaciens through characterization of biochemical and bacteriological aspects and 16S rDNA sequence. The plant height, stem diameter, root length and fresh weight of red pepper plants which were grown with inoculation of B. amyloliquefaciens MJ-3 were higher than those without inoculation. Especially the root weight of the inoculated red pepper plant increased by 44.3%, the content of endogenous plant hormone (CA$_1$) being 0.556 ng/g (dry weight).

본 연구에서는 가지과 작물의 근권에서 유용한 균주를 분리하고 근권ㆍ길항ㆍ생육촉진미생물을 선별ㆍ제제화하고 상토에 첨가하여 미생물제제 혼입 상토를 제조한 후 고추 플러그묘의 초기 생장에 미치는 영향을 조사하였다. 근권미생물로는 가지과 작물의 뿌리 및 재배 토양 등 385점의 균원시료로부터 540여점을 분리한 후, 가지과 작물에 공통적으로 발생되는 Alternaria solani, Botrytis cinerea, Fusarium oxysporium, Phytophthora capsici, Sclerotinia sclerotiorum 등의 병원균에 높은 길항력을 가진 미생물 20여종을 선발하고, 이들 길항미생물 중에서 고추의 초기 생육을 촉진시키는 MJ-3균주를 최종 선발하였다. 근권ㆍ길항ㆍ생육촉진미생물인 분리주 MJ-3은 Bergey's manual of systematic bacteriology의 방법과 16S rDNA sequence법 등으로 조사하여 Bacillus amyloliquefaciens동정하였다. 제제화한 B. amyloliquefaciens를 상토에 첨하여 미생물 혼입 상토를 제조하였고, 이를 이용하여 재배한 고추 플러그 묘는 초장과 경경, 뿌리길이, 생체중량 등이 모두 대조구에 비해 소량 증가되었으나, 특히 뿌리의 생체중량이 대조구가 0.61g/plant인데 비해 시험구는 0.88g/plant로 44.3% 증가하였다. 미생물제제 첨가 상토에서 재배한 고추의 gib-berellin (GA$_1$) 함량은 0.559ng/g(건조중량)이었다.

Keywords

References

  1. Cornell Univ. Infor. Bull. v.43 Cornell peatlite mixes for commercial plant growing Boodley,J.W.;Jr.Shelake
  2. J. Appl. Bacteriol. v.35 Plant growth substances produced by micro-organisms of soil and rhizospopher Brown,M.E. https://doi.org/10.1111/j.1365-2672.1972.tb03721.x
  3. Appl. Biochem. Biotechnol. v.48 Biological control of fungal pathogens Chet,I.;J.Inbar https://doi.org/10.1007/BF02825358
  4. Phytochem. v.25 Biosynthesis of gibberellin A12- aldehyde, gibberellin A12 and their kaurenoid precursors from [¹⁴C] mevalonic acid in a cell-free system from immature seed of Phaseolus coccineus Colin,G.;N.Turnbull;A.Crozier;L.Schwenen;J.E.Graebe https://doi.org/10.1016/S0031-9422(00)94509-6
  5. Plant Physiol. v.108 Genetic regulation of development in Sorghum bicolor. Ⅸ. The $ ma_3^ R$ < /TEX> allele disrupts diurnal control of gibberellin biosynthesis Foster,K.R.;P.W.Morgan
  6. Phytochem. v.46 Endogenous gibberellins in foliage of tomato (Lycopersicon esculentum) Grunzweig,J.M.;H.D.Rabinowitch;J.Katan;M.Wodner;Y.Ben-Tal https://doi.org/10.1016/S0031-9422(97)00383-X
  7. Planta v.103 Isolation and identification of the gibberellins of Cucumis sativus and Cucumis melo Hemphill,D.D.;L.R.Baker;H.M.Sell https://doi.org/10.1007/BF00386846
  8. Kor. J. Microbiol. Biotechol. v.30 Chitinase production and Isolation of Serratia plymuthica AL-1 antagonistic to white rot fungi from Allium fistulosum roots Joo,G.J.;I.H.Lee;J.H.Kim
  9. Can J. Microbiol. v.34 Alteration of wheat root systems and nitrogen fixation by associative nitrogen-fixing bacteria measured under field conditions Kucey,R.H.N. https://doi.org/10.1139/m88-125
  10. Plant and Soil v.57 Establishment nitrogen fixing and phosphate solublizing bacteria in rhizosphere and their effct on yield and nutrient uptake of wheat crop Kundu,B.S.;A.C.Gaur https://doi.org/10.1007/BF02211682
  11. Planta v.189 Gibberellin biosynthesis in cell-free extracts from developing Cucurbita maxima embryos and the identification Lange,T.;P.Hedden;J.E.Graebe https://doi.org/10.1007/BF00194431
  12. J. Plant Growth Regul. v.17 Effect of gibberellin biosynthesis inhibitors on native gibberellin content, growth and floral initiation in Sorghum bicolor Lee,I.J.;K.R.Foster;P.W.Morgan https://doi.org/10.1007/PL00007034
  13. Can. J. Microbiol. v.51 Growth promotion of canola seedlings by a strain of Pesudomonas putida under gnotobiotic conditions Lifschitz,R.;J.W.Kleopper;M.Kozlowshi;C.Simonson;J.Carlson;E.M.Tipping https://doi.org/10.1139/w04-136
  14. Appl. Environ. Microbiol. v.66 16S rRNA gene-based detection of tetrachoroethene-dechlorinating desulfuromonase and dehaloccoides species Loffler,J.E.;Q.Sun;J.Li;J.Tiedje https://doi.org/10.1128/AEM.66.4.1369-1374.2000
  15. U.S. Patent #5,418,165 Cold tolerant Trichoderma McBeath,J.
  16. Annu. rev. Plant Physiol. v.37 Siderophores in relation to plant growth and disease Nielands,J.B.;S.A.Leong https://doi.org/10.1146/annurev.pp.37.060186.001155
  17. Appl. Environ. Microbiol. v.61 Tn 5-directed cloning of pqq genes from Pseudomonas fluorescens CHAO:mutational inactivation of the genes results in overproduction of the anotic pyluteorin Schnider,U.;C.Keel;C.Voisard;G.Defago;D.Haas
  18. Bergey's manual of systematic bacteriology v.2 Sneath,P.A.;N.S.Mair;M.E.Sharpe;J.G.Holt
  19. Phytochem. v.61 Mulberry anthracnose antagonists(iturins) produced by bacillus amyloliquefaciens RC-2 Syuntaro,H.;S.Yoshida;H.Sugie;H.Yada;Y.Fujii https://doi.org/10.1016/S0031-9422(02)00365-5
  20. U.S. Patent #4,988,586 Method for the prevention of Fusarium dieases and microorganisms used for the same Toyoda,H.;R.Utsumi
  21. U.S. Patent #4,479,936 Method for protecting the growth of plants employing mutant siderophore producing strains of Pseudomonas putida Vandenbergh,P.A.;C.F.Gonzalez

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