홍화씨로부터 Phytosterol 추출의 최적화 및 재구성지질의 합성

Optimization for the Phytosterol Extraction and Production of Structured Lipids from Safflower seed

  • 발행 : 2003.06.01

초록

반응표면분석에 의하여 홍화유 추출조건에 따른 campesterol, stigmasterol, $\beta$ -sitosterol 및 total sterol의 추출 최적화를 모니터링 하였으며, 추출온도(35∼75$^{\circ}C$, X$_1$), 추출시간(1∼11시간 X$_2$), 중심합성계획으로 추출조건을 최적화하였다. campesterol 최적 추출범위는 추출온도, 추출시간 및 시료의 전처리 가공온도가 각각 59.01$^{\circ}C$, 2.88시간, 75.04$^{\circ}C$ 이었다. 그러나 $\beta$-sitosterol, stigmasterol 및 total sterol 추출율 반응표면분석은 유의차가 없었다. 한편 홍화의 35$^{\circ}C$ 유지 추출수율은 평균 23.44% 이었으며 8$0^{\circ}C$ 추출조건에서는 평균 20.05% 이었다. 추출온도가 35$^{\circ}C$에서 8$0^{\circ}C$높아짐에 따라 홍화유의 추출온도에 따른 평균 총 tocopherol 함량이 0.172% 에서 0.207%으로 증가하였다. 지방산 분석 결과 linoleic acid의 함량은 70.24%이었다. 홍화유와 CLA를 이용한 재구성 지질 합성결과 24시간 반응 재구성 지질에 함유된 CLA 함량은 31.79mol% 이었다.

Response surface methodology was used for monitoring and optimizing the extraction conditions of campesterol, stigmasterol, ${\beta}$ -sitosterol, and total sterols from the safflower seed. The conditions of phytosterol extraction were optimized by using central composite design with the temperature(35∼75$^{\circ}C$, X$_1$), the time (1∼11hr, X$_2$), and the preheating temperature(60∼100$^{\circ}C$, X$_3$) as three variables. The extraction conditions for maximum campesterol content were 59.01$^{\circ}C$(X$_1$), 2.88hr(X$_2$), and 75.04$^{\circ}C$(X$_3$). But stigmasterol, ${\beta}$ -sitosterol and total sterols were not significantly different under designed extraction condition in this study. Besides, oil was extracted from safflower seed at various conditions and yields were 23.44% at 35$^{\circ}C$ and 20.05% at 80$^{\circ}C$, respectively. Total tocopherol content increased from 0.172% to 0.207% as the extraction temperature increased from 35$^{\circ}C$ to 80$^{\circ}C$. A structured lipids(SL) was synthesized enzymatically by extracted safflower oil and conjugated linoleic acid(CLA). After 24hr reaction, 31.79 mol% CLA was incorporated into the extracted safflower oil.

키워드

참고문헌

  1. Kim, J.H., Kwak, D.Y., Choi, M.S. and Moon, K.D. (1998) Comparison of the chemical compositions of korean and chinese safflower seed. Korean J. Food Sci. Technol., 30, 1203-1208
  2. Kang, M.H., Song, E.S., Chung, H.K., Shim, K.B., Kang,C.W., Ryu, Y.H. and Lee, J.B. (2001) Comparison of oxidation stability in sesame, corn and safflower oils. Kor. J. Intl. Agri., 13, 115-120
  3. Namba, T. (1986) Coloured illustrations of wakan-yaku, 1st ed., vol. 2, Hoikusha Publishing Co., Ltd., Osaka Japan.
  4. Baek, N.I., Bang, M.H., Song, J.C., Lee, S.Y. and Park, N.K. (1999) N-feruloylserotonin, antioxidative component from the seed Carthamus tinctorius L.. J. Korean Soc. agric. Chem. Biotechnol., 42, 366-368
  5. Kim, E.Y. (2001) Synthesis of stigmastanol as a serum cholesterol-lowering substance using Pd catalyst. Korean J. Biotechnol. Bioeng., 16, 76-81
  6. 김봉현 (2001) 식용유지 그 이용과 유지 식품. Naeha publishing company. pp. 88-91
  7. Lee, I.B., Choi, K.J., Yu, K.K. and Chang, K.W. (1992) Tocopherols and fatty acids in plant seeds from Korea. J. Korean Agric. Chem. soc., 35, 1-5
  8. Ha, Y.L. and Micheal, W.P. (1991) Naturally-occurring novel anticarcinogens : Conjugated dienoic derivatives of linoleic acid(CLA). J. Kor. Soc. Food Nutr., 20, 401-407
  9. Hur, S.J., Lee, J.I., Ha, Y.L., Park, G.B. and Joo, S.T.(2002) Biological activities of conjugated linoleic acid(CLA) and animal products. J. Anim. Sci. & Technol., (Kor) 44, 427-442 https://doi.org/10.5187/JAST.2002.44.4.427
  10. Lee, K.T. and Akoh, C.C. (1998) Structured lipids : synthesis and application. Food Rev. Int., 14, 17-34 https://doi.org/10.1080/87559129809541148
  11. Lee, K.T. and Akoh, C.C. (1996) Immobilized lipase-catalyzed production of structured lipids with eicosapentaenoic acid at specific positions. J. Am. Oil Chem. Soc., 73, 611-615 https://doi.org/10.1007/BF02518116
  12. 이기동, 이정은, 권중호. (2000) 식품공업에서 반응표면분석의 응용. 한국식품과학회. 33, 33-63
  13. 성내경 (1994) SAS/STAT 희귀분석/제3권 자유아카데미 서울, 한국
  14. Bae, D.K., Choi H.J., Son J.H., Park, M.H., Bae, J.H., An,B.J., Bae, M.J. and Choi, C. (2000) Optimization for the process of extracts of persimmon leaf using response surface methodology. J. Korean Soc. Agric. Chem. Biotechnol., 43, 218-224
  15. Jeong, Y.J., Lee, G.D. and Kim, K.S. (1998) Optimization for fermentation condition of persimmon vinegar using response surface methodology. Korean J. Food Sci. Technol., 30, 1203-1208
  16. Jeong, Y.J., Lee, M.H. and Lee, G.D. (1999) Monitoring for the process conditions of chitosan using response surface methodology. Korean J. Food Sci. Technol., 31,558-563
  17. Jeong, Y.J., Lee, M.H., Lee, G.D., Seo, J.H. and Kim, O.M. (2001) Establishment on the preparation condition of pumpkin honey Kochujang by response surface methodology. J. Korean Soc. Food Sci. Nutr., 30, 1102-1107