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Study on the Simultaneous Production of the Bacterial Cellulose and Vinegar by Gluconacetobacter persimmonis KJ145T

Gluconacetobacter Persimmonis KJ145T를 이용한 Bacterial Cellulose및 식초의 동시 생산에 관한 연구

  • 정용진 (계명대학교 식품가공학과) ;
  • 여수환 (계명대학교 전통미생물자원개발 및 산업화연구센터) ;
  • 이오석 (계명대학교 전통미생물자원개발 및 산업화연구센터)
  • Published : 2003.10.01

Abstract

The changes of component through simultaneous production of bacterial cellulose and vinegars by G. persimmonis KJ145$^{T}$ were examined. As a results, pH was decreased to 3.22 at 8 days of fermentation and total acidity showed 4.66 which was the highest at the 8 days of fermentation. Brix didn't show any changes during the fermentation period. Free sugars of fermentation broth were consist of fructose, glucose and sucrose. The fructose concentration of fermentation broth was maintained highly during fermentation period (until the final 10 days) without a remarkable decrease. The cell growth of G. persimmonis KJ145$^{T}$ was very rapidly increased from the 2 days of fermentation and increased most at the 4 days of fermentation. The productivity of bacterial cellulose was increased in proportion to the fermentation period. Malic acid, succinic acid and oxalic acid were detected as a organic acid of vinegar. The concentration of acetic acid was rapidly increased from the 2 days and reached highest concentration at 8 days. In conclusion, the results indicated that the 8 days was the optimal fermentation period to produce the bacterial cellulose and vinegar by G. persimmonis KJ145$^{T}$ simultaneously.

G. persimmonis KJ145$^{T}$ 를 사용하여 bacterial cellulose와 식초 동시 생산에 따른 성분변화를 조사하였다. 그 결과 pH는 배양 8일째에 3.22로 감소하는 경향이었으며, 총산은 8일째에 가장 높은 4.66으로 나타났다. Brix는 배양기간 동안 거의 변화가 없었으며, 유리당은 fructose, glucose 및 sucrose로 구성되어 있고 fructose는 배양 종료 후, 10일째에도 거의 감소하지 않고 높게 나타났다. G. Persimmonis KJ145$^{T}$ 균체는 배양 2일부터 급격히 증가하여 4일에 가장 높게 나타났으며, bacterial cellulose는 배양기간이 길수록 증가하는 경향을 나타내었다. 발효액의 유기산은 malic acid, succinic acid, oxalic acid 등이 검출되었으며, acetic acid는 발효 2일째부터 급격히 증가하여 8일째에 가장 높게 생성되었다. 따라서 bacterial cellulose와 초산을 동시에 생산하기 위한 최적배양기간은 8일 정도로 나타났다.

Keywords

References

  1. Kwon SH, Jeong EJ, Lee GD, Jeong YJ. 2000. Preparation method of fruit vinegars by two stage fermentation and beverage including vinegar. Food Industry and Nutrition 5: 18-24.
  2. Jeong YJ, Shin SR, Kang MJ, Seo CH, Won CY, Kim KS. 1996. Preparation and quality evaluation of the quick fermented persimmon vinegar using deteriorated sweet persimmon. J East Asian of Dietary Life 6: 221-227.
  3. Jeong YJ, Seo JH, Lee GD, Park NY, Choi TH. 1999. The quality comparison of apple vinegar by two stages fermentation with commercial apple vinegar. J Korean Soc Food Sci Nutr 28: 353-358.
  4. Seo JH, Lee GD, Jeong YJ. 2001. Optimization of the vinegar fermentation using concentrated apple juice. J Korean Soc Food Sci Nutr 30: 460-465.
  5. Jeong YJ, Lee IS. 2000. A view of utilizing cellulose produced by Acetobacter bacteria. Food Industry and Nutrition 5: 22-29.
  6. Jonas R, Farah LF. 1998. Production and application of microbial cellulose. Polym Degrad Stab 59: 101-106. https://doi.org/10.1016/S0141-3910(97)00197-3
  7. Vandamme EJ, Baets SD, Vanbaelen A, Joris K, Wulf PD. 1998. Improved production of bacterial cellulose and its application potential. Polym Degrad Stab 59: 93-99. https://doi.org/10.1016/S0141-3910(97)00185-7
  8. Klemm D, Schumann D, Udhardt U, Marsch S. 2001. Bacterial synthesized cellulose-artificial blood vessels for microsurgery Prog Polym Sci 26: 1561-1603. https://doi.org/10.1016/S0079-6700(01)00021-1
  9. Jeong YJ, Lee IS. 2000. A view of utilizing cellulose produced by Acetobacter bacteria. Food Industry and Nutrition 5: 22-29.
  10. Lee OS, Jeong YJ. 2001. Industrial application and biosynthesis of bacterial cellulose. Food Industry and Nutrition 6: 10-14.
  11. Lee OS, Jang SY, Jeong YJ. 2002. Culture condition for the production of bacterial cellulose with Gluconacetobacter persimmonus KJ145. J Korean Soc Food Sci Nutr 31: 572-577. https://doi.org/10.3746/jkfn.2002.31.4.572
  12. Hestrin S, Schramm M. 1954. Synthesis of cellulose by Acetobacter xylinum. 2. Preparation of freeze dried cells capable of polymerizing glucose to cellulose. Biochem J 58: 345-352.
  13. Jeong YJ, Seas KI, Kim KS. 1996. Physiochemical properties of marketing and intensive persimmon vinegars. J East Asian of Dietary Life 6: 355-363.
  14. Cho RK, Sohn MR. 1998. New approach of nondestructive evaluation for sweetness in apple fruit using near-infrared spectroscopy. J Kor Soc Hort Sci 39: 745-750.