Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Photosynthetic Activity, and Lipid and Hydrocarbon Production by Alginate-Immobilized Cells of Botryococcus in Relation to Growth Phase

  • Published : 2003.10.01
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Abstract

Whole-cell immobilization of the hydrocarbon rich microalgae, Botryococcus braunii and B. protuberans, in alginate beads under air-lift batch cultures resulted in a significant increase in chlorophyll, carotenoid, dry weight, and 1ipid contents at stationary and resting growth phases, as compared to free cells. Photosynthetic activity in both the species, of Botryococcus was enhanced, relative to free cells, at any growth phase of cultures. Immobilization exerted a protective influence on ageing of the cultures as reflected by higher chlorophyll and dry weight contents. Entrapment also stabilized the chlorophyll and carotenoid contents even at stationary and resting phases as compared to free cells in both the species.

Keywords

References

  1. Appl. Microbiol. Biotechnol. v.23 Immobilization of Botryococcus braunii in alginate. Influence on chlorophyll content, photosynthetic activity and degeneration during batch cultures Bailliez,C.;C.Largeau;C.Berkaloff;E.Casadevall
  2. Appl. Microbiol. Biotechnol. v.23 Growth and hydrocarbon production of Botryococcus braunii immobilized in calcium alginate gel Bailliez,C.;C.Largeau;E.Casadevall
  3. Appl. Microbiol. Biotechnol. v.29 Photosynthesis, growth and hydrocarbon production of Botryococcus braunii immobilized by entrapment and adsorption in polyurethane foams Bailliez,C.;C.Largeau;E.Casadevall;L.W.Yang;C.Berkaloff
  4. Solar Energy R&D Eur. Community Series $D_2$ Long term stabilization of photobiological activities of immobilized algae. Photoproduction of hydrogen by immobilized adapted Scenedesmus cells Brouers,M.;F.Collard;J.Jeanfils;R.Loudeche
  5. Phil. Trans. R. Soc. London B v.300 Immobilized cells Bucke,C. https://doi.org/10.1098/rstb.1983.0011
  6. Biochem. Bioeng. v.27 Studies on batch and continuous cultures of Botryococcus braunii: Hydrocarbon production in relation to physiological state, cell ultrastructure and phosphate nutrition Casadevall,E.;D.Dif;C.Largeau;C.Gudin;D.Chaumont;D.Desanti https://doi.org/10.1002/bit.260270312
  7. Biochem. Bioeng. v.38 Continuous acetone-butanol-ethanol fermentation using immobilized cells of Clostridium acetobutylicum in packed bed reactor and integration with product removal by pre-evaporation Friedl,A.;N.Qureshi;I.S.Maddox https://doi.org/10.1002/bit.260380510
  8. Biotech. 84 : The World Biotech Report 1984 v.1 Direct bioconversion of solar energy into organic chemicals Gudin,C.;A.Bernard;D.Chaumont;C.Thepenier;T.Hardy
  9. Adv. Biotechnol. Processes v.6 Bioconversion of solar energy into organic chemicals by microalgae Gudin,C.;C.Thepenier
  10. Techniques in Lipidology Isolation, analysis and identification of lipids Kates,M.;T.S.Work(ed.);D.Work(ed.)
  11. Biochemistry of the Algae and Cyanobacteria v.28 The biotechnology of microalgae and cyanobacteria Kerby,N.W.;W.D.P.Stewart;L.J.Rogers(ed.);J.R.Gallon(ed.)
  12. Biotechnol. Letters v.4 Sustained ammonia production by immobilized filaments of the nitrogen fixing cyanobacterium Anabaena 27893 Musgrave,S.C.;N.W.Kerby;C.A.Codd;W.D.P.Stewart https://doi.org/10.1007/BF00141979
  13. J. Gen. Physiol. v.39 Relationship between pigments content and photosynthetic characteristics in a blue-green alga Myers,J.;W.A.Kratz https://doi.org/10.1085/jgp.39.1.11
  14. Angew Chem. v.21 Immobilized biomaterials: Techniques and applications Sharma,B.P.;L.F.Bailey;R.A.Messing https://doi.org/10.1002/anie.198208371
  15. Ph.D. Thesis, Banaras Hindu University Physiological and Biochemical Studies on the Alga, Botryococcus Singh,Y.
  16. World J. Microbiol. Biotechnol. v.8 Lipid and hydrocarbon production by Botryococcus spp. under nitrogen limitation and anaerobiosis Singh,Y.;H.D.Kumar https://doi.org/10.1007/BF01195829
  17. Phykos v.33 no.I;II Growth of Botryococcus spp. in improved medium Singh,Y.;H.D.Kumar
  18. Physiol. Plant v.63 Cytological and physiological behaviour of Euglena gracilis cells entrapped in a calcium alginate gel Tamponnet,C.;F.Costantino;J.N.Barbotin;R.Calvayrac https://doi.org/10.1111/j.1399-3054.1985.tb04265.x
  19. Experientia v.38 Microorganisms as hydrocarbon producers Tornabene,T.G. https://doi.org/10.1007/BF01944525
  20. Appl. Biochem. Biotechnol. v.8 Botryococcus braunii: An unusual hydrocarbon producing alga Wolf,F.R. https://doi.org/10.1007/BF02778262