We have shown in Part I [1] of this study that medical needle-stick injuries are causing serious health problems to healthcare personnel and other professionals that require the attention of healthcare and textile researchers to develop new protective systems. Responding to such need, a needle force measurement device that is capable of measuring dynamic forces experienced by medical needles during needle penetration through protective articles was developed and described in part I. This paper reports the results of evaluation of protective woven fabrics from high performance fibers and standard and bifid medical needles using the force measurement system. The woven fabrics varied in cover factor, number of layers, and orientation angle. Standard and bifid needles with different gap widths were used to evaluate the resistance of the fabric to needle penetration.