Extract Characteristics of Old Pumpkin on Enzyme Treatment

효소제 처리에 따른 늙은 호박의 추출 특성

  • Published : 2003.09.01

Abstract

In the present study, we investigated the quality characteristic of old pumpkin extract treated with enzymes. As a results, all the groups treated with pectinase were better in quality characteristic than control group and the group treated with 0.15%(w/w) pectinase was specially great. All the groups treated with simultaneous pectinase and cellulase were higher in the extraction rate than the groups treated with pectinase or cellulase. The experimental groups were divided into non-treated control(I) and three treatment groups(II- IV) for optimum condition of enzyme treatment. The II and IV groups were treated with 0.15%(w/w) pectinase and 0.15%(w/w) cellulase, respectively, and the ill group was treated with both 0.15%(w/w) petinase and 0.05%(w/w) cellulase. Yield for old pumpkin extract of the III group (86.94%) was higher than that of other groups, but there were no significant difference among the groups in soluble solide content and pH of the extract. Reducing sugar and total sugar contents in the ill group were 2.81% and 4.60%, respectively. Total carotene content in the II group (5.36 mg%) was higher than other groups. Old pumpkin extracts in all the groups showed nitrite-scavenging ability to pH 1.2, 3.0 and 4.0. Total free amino acid content in the III group (176.7 mg%) was higher than other groups. Citrulline contents in the II and III groups were detected 1.66 and 1.41 mg%, respectively but the contents in other groups were not detected.

본 연구에서는 늙은 호박의 효소처리 조건에 따른 추출특성을 조사하였다. 그 결과, pectinase에 의한 영향에서는 효소제 처리를 한 모든 구가 대조구보다 품질 특성이 우수하였으며, 특히 0.15%(w/w) pectinase 처리구의 품질특성이 가장 높게 분석되었다. 혼합 효소제 처리에서는 pectinase와 cellulase의 단독 처리구에 비해 pectinase와 cellulase의 혼합 효소제의 추출율이 높게 나타났다. 최적 효소제 처리조건 설정을 위한 늙은 호박의 추출조건(I~IV)에서 추출물의 수율은 pectinase와 cellulase를 혼합 처리한 구(III)에서 86.94%로 가장 높게 나타났으며, 가용성 고형분 및 pH는 모든 처리구에서 비슷한 경향을 보였다. 환원당과 전당 함량은 각각 2.81, 4.60%로 나타난 효소제 혼합 처리구(III)가 높았으며 total carotene은 pectinase 처리구가 5.36mg%로 높은 함량을 나타내었다. 모든 처리구에 대한 아질산염소거능 결과는 pH 6.0을 제죄한 pH 1.2, 3.0, 4.0에서 소거능이 있는 것으로 나타났고 유리 아미노산 분석에서는 총 함량이 176.85, 171.89 mg%인 II, III 처리구가 무처리구에 비해 높은 함량으로 분석되었으며 citrulline은 I, IV 처리구에서는 분석되지 않았으나 II, III 처리구에서는 1.61, 1.4l mg%로 분석되었다. 이상의 결과로 늙은 호박의 추출조건으로는 무처리구보다는 효소제를 처리한 구에서 높은 수율 및 품질의 우수성이 인증되었으며 그 중 III 처리구인 pectinase와 cellulase(0.15 : 0.05, %)를 혼합하여 추출함으로써 추출율 및 품질특성이 우수한 추출물을 얻을 수 있을 것으로 생각된다.

Keywords

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