Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Influence of Transition-Metal Cofactors on the Reductive Dechlorination of Polychlorinated Biphenyls (PCBs)

  • Kwon, O-Seob (School of Environmental Science and Engineering, and Nakdong River Environmental Research Center, Inje University) ;
  • Kim, Young-Jin (School of Environmental Science and Engineering, and Nakdong River Environmental Research Center, Inje University) ;
  • Cho, Kyung-Je (School of Environmental Science and Engineering, and Nakdong River Environmental Research Center, Inje University) ;
  • Lee, Jin-Ae (School of Environmental Science and Engineering, and Nakdong River Environmental Research Center, Inje University) ;
  • Kim, Young-Eui (School of Environmental Science and Engineering, and Nakdong River Environmental Research Center, Inje University) ;
  • Hwang, In-Young (School of Environmental Science and Engineering, and Nakdong River Environmental Research Center, Inje University) ;
  • Kwon, Jae-Hyun (School of Environmental Science and Engineering, and Nakdong River Environmental Research Center, Inje University)
  • Published : 2003.09.01
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Abstract

To enhance the reductive dechlorination of polychlorinated biphenyls (PCBs) under anaerobic conditions, we examined the adjunctive effects of cobalt (Co) and nickel (Ni), which are the central metals of transition-metal cofactors of coenzyme F$\_$430/ and vitamin B$\_$12/, respectively, on the dechlorination of Aroclor 1248. After 32 weeks of incubation, the average numbers of chlorines per biphenyl in culture vials supplemented with 0.2, 0.5, and 1.0 mM of Co reduced from 3.88 to 3.39, 2.92, and 3.28, respectively. However, the numbers of chlorine after supplementing with Ni decreased from 3.88 to 3.43, regardless of the Ni concentrations. The observed congener distribution patterns of all vials with different conditions were similar to the pattern produced by the dechlorination process of H' after 21 weeks of incubation, and these patterns were unchanged up to week 32, except for vials supplemented with 0.5 and 1.0 mM of Co. In vials containing 0.5 mM of Co, meta-rich congeners, such as 25/ 25-,24/25-, and 25/23-chlorobiphenyls (CBPs), which were found as accumulated products of dechlorination in other conditions, were further dechlorinated, and 25/2-, 24/2-, and 2/2-CBPs were concomitantly increased after 32 weeks of incubation. In this case, the congener distribution was similar to the dechlorination pattern of process M. From these results, we suggested that the enrichment of cultures with Co might stimulate the growth of specific populations of meta-dechlorinators, and that populations might promote a change in the dechlorination process from H' to M, which is known to be less effective on the dechlorination of the more highly chlorinated congeners of PCBs.

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References

  1. Appl. Environ. Microbiol. v.56 Effects of metals on Streptomyces coelicolor growth and actinorhodin production Abbas,A.S.;C.Edwards
  2. Environ. Sci. Technol. v.27 Factors influencing the rate of polychlorinated biphenyl dechlorination in Hudson River sediments Abramowicz,D.A.;M.J.Brennan;H.M. Van Dort;E.L.Gallagher https://doi.org/10.1021/es00043a011
  3. Biodegradation and bioremediation Alexander,M.
  4. Microbiol. Rev. v.43 Methanogens: reevaluation of a unique biological group Balch,W.E.;G.E.Fox;L.J.Magrum;C.R.Woese;R.S.Wolfe
  5. Microbial transformation and degradation of toxic organic chemicals Microbial reductive dechlorination of polychlorinated biphenyls Bedard,D.L.;J.F.QuensennⅢ;L.Y.Young(ed.);C.E.Cerniglia(ed.)
  6. Environ. Sci. Technol. v.30 Stimulation of microbial para-dechlorination of polychlorinated biphenyls that have persisted in Housatonic River sediment for decades Bedard,D.L.;S.C.Bunnell;L.A.Smullen https://doi.org/10.1021/es950463i
  7. J. Bacteriol. v.146 Plasmid specifying total degradation of 3-chlorobenzoate by a modified ortho pathway Chatterjee,D.K.;S.T.Kellogg;S.Hamadam;A.M.Chakrabarty
  8. Can. J. Fish. Aquat.Sci. v.57 Biotransformation of polychlorinated biphenyls in St. Lawrence River sediments reductive dechlorination and dechlorinating microbial populations Cho,Y.C.;J.Kim;R.C.Sokol;G.Y.Rhee https://doi.org/10.1139/cjfas-57-S1-95
  9. FEMS Microbiol. Ecol. v.42 Enhancement of microbial PCB dechlorination by chlorobenzoates, chlorophenols, and chlorobenzenes Cho,Y.C.;E.B.Ostrofsky;R.C.Sokol;R.C.Frohnhoefer;G.Y.Rhee https://doi.org/10.1111/j.1574-6941.2002.tb00994.x
  10. Environ. Microbiol. v.3 Identification of a microorganism that links its growth to the reductive dechlorination of 2,3,5,6,-chlorobiphenyl Cutter,L.A.;J.E.Watts;K.R.Sowers;H.D.May https://doi.org/10.1046/j.1462-2920.2001.00246.x
  11. Arch. Microbiol. v.148 Occurrence of corrinoid-containing membrane proteins in anaerobic bacteria Danler,W.;H.Schulz;G.Diekert;H.Konig;G.Fuchs https://doi.org/10.1007/BF00429647
  12. Environ. Sci. Technol. v.33 Use of halogenated benzonates and other halogenated aromatic compounds to stimulate the microbial dechloronation of PCBs Deweerd,K.A.;D.L.Bedard https://doi.org/10.1021/es9812498
  13. J. Bacteriol. v.161 Transposon mutagenesis and cloning analysis of the pathways for degradation of 2,4-dichlorophenoxyacetic acid and 3-chlorobenzoate in Alcligenes eutrophus JMP134(pJP4) Don,R.H.;A.J.Weightman;H.J.Knackmuss;K.N.Timmis
  14. Contaminated sedimants:Lectures on environmental aspects of particle-associated chemicals in aquatic systems Forstner,U.
  15. Environ. Sci. Technol. v.25 Reductive dechlorination catalyzed by bacterial transition-metal coenzyme Gantzer,C.J.;L.P.Wackett https://doi.org/10.1021/es00016a017
  16. Environ. Toxicol. Chem. v.18 Interactions of polychlorinated biphenyl-dechlorinating microorganisms with methanogens and sulfate reducers Kim,J.;G.Y.Rhee https://doi.org/10.1897/1551-5028(1999)018<2696:RDOPBI>2.3.CO;2
  17. J. Microbiol. v.39 Effect of moisture content on reductive dechlorinating of polychlorinated biphenyls and population dynamics of dechlorinating microorganisms Kwon,O.S.;Y.E.Kim;J.G.Park
  18. Appl. Environ. Microbiol. v.58 Subculturing of a polychlorinated biphenyl-decholorinating anaerobic enrchment on solid media May,H.D.;A.W.Boyle;W.A.Prince Ⅱ;C.K.Blake
  19. J. Bacteriol. v.162 Alcaligenes eutrophus CH34 is a facultative chemolithotroph with plasmid-bound resistance to heavy metals Mergeay,M.D.;H.G.Nies;J.Schlegel;G.P.Charles;F. Van Gijsegem
  20. Environ. Toxical. Chem. v.12 Anaerobic dechlorination of Aroclor 1242 as affected by some environmental conditions Rhee,G.Y.;B.Bush;C.M.Bethoney;A.DeNucci;H.M.Oh;R.C.Sokol https://doi.org/10.1897/1552-8618(1993)12[1033:ADOAAA]2.0.CO;2
  21. Environ. Sci. Technol. v.27 Long-term study of the anaerobic dechlorination of Aroclor 1254 with and without biphenyl enrichment Rhee,G.Y.;R.C.Sokol;B.Bush;C.M.Bethoney https://doi.org/10.1021/es00041a015
  22. Environ. Toxical. Chem. v.20 Kinetics of polychlorinated biphenyl dechlorination and growth of dechlorinating microorganisms Rhee,G.Y.;R.C.Sokol;C.M.Bethoney;Y.C.Cho;R.C.Frohnhoefer;T.Erkkila https://doi.org/10.1897/1551-5028(2001)020<0721:KOPBDA>2.0.CO;2
  23. Environ. Health Perspect. v.100 Toxicity, structuture, function, relationship, and human and environmental health impacts of polychlorinated biphenyls-Progress and problems Safe,S. https://doi.org/10.2307/3431532
  24. Appl. Environ. Microbiol. v.60 Regiospecificity of chlorophenol reductive dechlorination by vitamin $B_{12s}$ Smith,M.H.;S.L.Woods
  25. Appl. Environ. Microbiol. v.59 Construction and characterization of heavy metal-resistant haloaromatic-degrading Alcaligenes eutrophus strains Springael,D.;L.Diels;L.Hooyberghs;S.Kreps;M.Mergeay
  26. Anal. Chem. v.51 Sequential extraction procedure for the speciation of particulate trace metals Tessier,A.;P.G.C.Campbell;M.Bisson https://doi.org/10.1021/ac50043a017
  27. Appl. Environ. Microbiol. v.61 Evidence for para dechlorination of polychlorobiphenyls by methanogenic bacteria Ye,D.;J.F.Quensen Ⅲ;J.M.Tiedje;S.A.Boyd