Restorative Dentistry and Endodontics

  • 제28권4호
  • /
  • Pages.303-313
  • /
  • 2003
  • /
  • 2234-7658(pISSN)
  • /
  • 2234-7666(eISSN)
대한치과보존학회 (The Korean Academy of Conservative Dentistry)
권호 표지
DOI QR코드

DOI QR Code

근관감염세균의 분포에 관한 연구

DISTRIBUTION OF ORAL PATHOGENS IN INFECTIONS OF ENDODONTIC ORIGIN

  • 김승윤 (경희대학교 대학원 치의학과 치과보존학교실) ;
  • 최호영 (경희대학교 대학원 치의학과 치과보존학교실) ;
  • 박상혁 (경희대학교 대학원 치의학과 치과보존학교실) ;
  • 최기운 (경희대학교 대학원 치의학과 치과보존학교실)
  • Kim, Seung-Yoon (Department of Conservative Dentistry, Division of Dentistry, Graduate School Kyung-Hee University) ;
  • Choi, Ho-Young (Department of Conservative Dentistry, Division of Dentistry, Graduate School Kyung-Hee University) ;
  • Park, Sang-Hyuk (Department of Conservative Dentistry, Division of Dentistry, Graduate School Kyung-Hee University) ;
  • Choi, Gi-Woon (Department of Conservative Dentistry, Division of Dentistry, Graduate School Kyung-Hee University)
  • 발행 : 2003.07.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

It has been documented that periodontopathic bacteria are also implicated in endodontic infections. 168 rDNA gene-directed PCR was to examine the prevalence of periodontopathic bacteria including Actinobacillus actinomycetemcomitans (Aa), Prevotella intermedia (Pi), Prevotella nigrescens (Pn), Porphyromonas gingivalis (Pg), Porphyromonas endodontalis (Pe), and Treponema denticola (Td) in the root canals of 36 endodontically infected teeth having apical lesions with or without clinical symptoms like pain, swelling, and fistula. 1. In 36 infected root canals, most frequently detected bacterial species was Pg (61.1%), followed by Td (52.8%) and Pe (38.9%). 2. Of 36 infected root canals, Aa was detected in 6 canals (16.7%) of the teeth, all of which showed clinical symptoms. 3. Of 36 infected root canals, Pi and Pn were found in 4 03.9%) and 5 (33.3%), respectively. Notably, prevalence of Pn in the symptomatic teeth was 50.0%. 4. One of black-pigmented anaerobic bacteria (BPB) including Pi, Pn, Pe, and Pg was detected in all of the teeth that showed pain or especially swelling but not fistula. It was, however, found that prevalence of BPB in the asymptomatic teeth or the teeth with fistula was only 40%. 5. Pe and Pg were detected in the teeth regardless of the presence or absence of symptoms. 6. Td was detected in the teeth regardless of the presence or absence of symptoms. High prevalence of BPB in the symptomatic teeth but low in the asymptomatic teeth suggests that BPB may play an important role in the pathogenesis of periapical lesions.

키워드

참고문헌

  1. Miller W.D. : Microorganisms of the human mouth. Philadelphia, S.S. White Dental Co., 1890
  2. Dahlen G. and Moller A.J.R: Microbiology of endodontic infections. In J. Slots and M.A. Taubman (eds.), Contemporary oral microbiology and immunology, St. Louis, Mosby-Year Book, Inc., p.444-475. 1992
  3. Kettering J.D. and Torabinejad M.: Microbiology and immunology. In S. Cohen and R.C. Burns (eds.), Pathways of the pulp, St. Louis, Mosby-Year Book, Inc., p.363-376. 1994
  4. Sjogren U, et. al.: Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. Int Endod J, 30:297-306. 1997 https://doi.org/10.1111/j.1365-2591.1997.tb00714.x
  5. Haapasalo M.: Black-pigmented Gram-negative anaerobes in endodontic infections. FEMS Immunol Med Microbiol, 6:213-218, 1993 https://doi.org/10.1111/j.1574-695X.1993.tb00329.x
  6. Sundqvist G.: Bacteriological studies of necrotic dental pulps. Thesis. Umea University, Odontology, Dissertation No.7, 1976
  7. Kobayashi T., Hayash. A., Yoshikawa R., Okuda K. and Hara K.: The microbial flora from root canals and periodontal pockets of non-vital teeth associated with advanced periodontitis. Int Endod J, 23:100-106, 1990 https://doi.org/10.1111/j.1365-2591.1990.tb00847.x
  8. Van Winkelhoff A.J., Carlee A.W. and de Graaff J.: Bacteroides endodontalis and other black-pigmented Bacteroides species in odontogenic abscesses. Infect Immun, 49:494-497, 1985
  9. Haapasalo M.: Bacteroides spp. in dental root canal infections. Endod Dent Traumatol, 5:1-10, 1989 https://doi.org/10.1111/j.1600-9657.1989.tb00330.x
  10. Sundqvist G., Johansson E. and Sjogren U.: Prevalence of black-pigmented Bacteroides species in root canal infections. J Endod, 15: 13-19. 1989 https://doi.org/10.1016/S0099-2399(89)80092-5
  11. Tanner A. and Stillman N.: Oral and dental infections with anaerobic bacteria: clinical features, predominant pathogens, and treatment. Clin Infect Dis, 16(suppl.4): S304-S309, 1993 https://doi.org/10.1093/clinids/16.Supplement_4.S304
  12. Baumgarten J.C. and Falkler W.A. Jr.: Reactivity of IgG from explant cultures of periapical lesions with implicated microorganisms. J Endod, 17:207-212, 1991 https://doi.org/10.1016/S0099-2399(06)81922-9
  13. Tanner A. and Stillman N.: Oral and dental infections with anaerobic bacteria: clinical features, predominant pathogens and treatment. Clin Infect Dis,16(Suppl.):S304-S309, 1993 https://doi.org/10.1093/clinids/16.Supplement_4.S304
  14. Haapasalo M., Ranta H., Ranta K. and Shah H.: Black-pigmented Bacteroides spp. in human apical periodontitis. Infect Immun, 53:149-153, 1986
  15. Chen H.: The correlation of black-pigmented Bacteroides spp. to symptoms associated with apical periodontitis. Chung-Hua Kou Chiang i Hsueh Chinese J Stomatol, 20:70-72, 1991
  16. Griffee M.B., Patterson S.S., Miller C.H ., Kafrawy A.H. and Newton C.W.: The relationship of Bacteroides melaninogenicus to symptoms associated with pulpal necrosis. Oral Surg Oral Med Oral Pethol. 50:457-461, 1980 https://doi.org/10.1016/S0030-4220(80)80015-6
  17. Yoshida M., Fukushima H., Yamamoto K., Ogawa K., Toda T. and Sagawa H.: Correlation between clinical symptoms and microorganisms isolated from root canals with periapical lesions. J Endod, 13:24-28, 1987 https://doi.org/10.1016/S0099-2399(87)80088-2
  18. Hahn C.-L., Falkler W.A. Jr. and Minah G.E.: Correlation between thermal sensitivity and microorganisms isolated from deep carious dentin. J Ended, 19:26-30, 1993 https://doi.org/10.1016/S0099-2399(06)81037-X
  19. Hashioka K., Suzuki K., Yoshida T., Nakane A., Horiba N. and Nakamura H.; Relationship between clinical symptoms and enzyme-producing bacteria isolated from infected root canals. J Endod, 20:75-77, 1994 https://doi.org/10.1016/S0099-2399(06)81185-4
  20. Gomes B.P., Drucker D.B. and Lilley J.D.: Associations of specific bacteria with some endodontic signs and symptoms. Int Endod J, 27:291-298, 1994 https://doi.org/10.1111/j.1365-2591.1994.tb00271.x
  21. Gomes B.P., Lilley J.D. and Drucker D.B.: Clinical significance of dental root canal microflora. J Dent, 24:47-55, 1996 https://doi.org/10.1016/0300-5712(95)00042-9
  22. Olsen G,J.: Microbial ecology. Variation among the masses. Nature, 345:20, 1990 https://doi.org/10.1038/345020a0
  23. Ward D.M., Weller R. and Bateson M.M.: 16S rRNA sequences reveal numerous uncultured microorganisms in a natural community. Nature, 345:63-65, 1990 https://doi.org/10.1038/345063a0
  24. Amann R.I., Ludwig W. and Schleife K.H.: Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev, 59:143-169, 1995
  25. Siqueira J.F.Jr., Rocas I.N., Souto R., de Uzeda M. and Colombo A.P.: Checkerboard DNA-DNA hybridization analysis of endodontic infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 89:744-748, 2000 https://doi.org/10.1067/moe.2000.106576
  26. Martin F.E., Nadkarni M.A., Jacques N.A. and Hunter N.: Quantitative microbiological study of human carious dentine by culture and real-time PCR: association of anaerobes with histopathological changes in chronic pulpitis. J Clin Microbiol, 40:1698-1704, 2002 https://doi.org/10.1128/JCM.40.5.1698-1704.2002
  27. Jung I.Y., Choi B., Kum K.Y., Yoo Y.J ., Yoon T.C., Lee S.J. and Lee C.Y.: Identification of oral spirochetes at the species level and their association with other bacteria in endodontic infections. Oral Surg Oral Med Oral Pathol Oral Radiol Ended, 92:329-334, 2001 https://doi.org/10.1067/moe.2001.117263
  28. Baumgartner J.C., Watts C.M. and Xia T.: Occurrence of Candida albicans in infections of endodontic origin. J Endod., 26:695-698, 2000 https://doi.org/10.1097/00004770-200012000-00003
  29. Ashimoto A., Chen C., Bakker I. and Slots J.: Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. Oral Microbiol Immunol, 11:266-273, 1996 https://doi.org/10.1111/j.1399-302X.1996.tb00180.x
  30. Bogen G. and Slots J.: Black-pigmented anaerobic rods in closed periapical lesions. Int Endod J. 32: 204-210, 1999 https://doi.org/10.1046/j.1365-2591.1999.00216.x
  31. Conrads G., Gharbia S.E., Gulabivala K., Lampert F.and Shah H.N.: The use of a 16S rDNA directed PCR for the detection of endodontopathogenic bacteria. J Ended, 23:433-438, 1997 https://doi.org/10.1016/S0099-2399(97)80297-X
  32. Goncalves R.B. and Mouton C.: Molecular detection of Bacteroides forsythus in infected root canals. J Endod, 25:336-340, 1999 https://doi.org/10.1016/S0099-2399(06)81167-2
  33. Siqueira J.F.Jr., Rocas I.N., Favieri A. and Santos K.R.: Detection of Treponema denticola in endodontic infections by 16S rRNA gene-directed polymerase chain reaction. Oral Microbiol Immunol, 15:335-337, 2000 https://doi.org/10.1034/j.1399-302x.2000.150512.x
  34. Siqueira J.F.Jr., Rjcas I.N., Oliveira .JC. and Santos K.R.: Detection of putative oral pathogens in acute periradicular abscesses by 16S rDNA-directed polymerase chain reaction. J Endod, 27:164-7, 2001 https://doi.org/10.1097/00004770-200103000-00004
  35. Siqueira J.F.Jr., Rocas I.N., Favieri A., Oliveira J.C. and Santos KR.: Polymerase chain reaction detectionof Treponema denticola in endodontic infections within root canals. Int Endod J. 34:280-284, 2001 https://doi.org/10.1046/j.1365-2591.2001.00382.x
  36. Rupf S., Kannengiesser S., Merte K., Pfister W., Sigusch B. and Eschrich K.: Comparison of profiles of key periodontal pathogens in periodontium and endodontium. Endod Dent Traumatol, 16:269-275, 2000 https://doi.org/10.1034/j.1600-9657.2000.016006269.x
  37. Socransky S.S., Jaffajee A.D., Cugini M.A., Smith C. and Kent R.L.Jr.: Microbial complexes in subgingival plaque. J Clin Periodontol, 25:134-144, 1998 https://doi.org/10.1111/j.1600-051X.1998.tb02419.x
  38. Rocas I.N., Siqueira J.F.Jr., Santos K.R. and Coelho A.M.: 'Red complex' (Bacteroides forsythus, Porphyromonas gingivalis, and Treponema denticola)in endodontic infections: a molecular approach. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 91:468-471, 2001 https://doi.org/10.1067/moe.2001.114379
  39. Sunde P.T., Tronstad L., Eribe E.R., Lind P.O. and Olsen I.: Assessment of periradicular microbiota by DNA-DNA hybridization. Endod Dent Traumatol, 16:191-196, 2000 https://doi.org/10.1034/j.1600-9657.2000.016005191.x
  40. Jansson L., Ehnevid H., Blomlof L., Weintraub A. and Lindskog S.: Endodontic pathogens in periodontal disease augmentation. J Clin Periodontol, 22:598-602, 1995 https://doi.org/10.1111/j.1600-051X.1995.tb00811.x
  41. Jansson L. Ehnevid H., Lindskog S. and Blomlof L.: The influence of endodontic infection on progression of marginal bone loss in periodontitis. J Clin Periodoniol, 22:729-734, 1995 https://doi.org/10.1111/j.1600-051X.1995.tb00254.x
  42. Baumgartner J.C., Watkins B.J, Bae K.S. and Xia T.: Association of black-pigmented bacteria with endodontic infections. J Endod, 25:413-415, 1999 https://doi.org/10.1016/S0099-2399(99)80268-4
  43. Fouad A.F., Barry J., Caimano M., Clawson M., Zhu Q., Carver R., Hazlett K. and Radolf J.D.: PCR-based identification of bacteria associated with endodontic infections. J Clin Microbiol, 40:3223-3231, 2002 https://doi.org/10.1128/JCM.40.9.3223-3231.2002
  44. Siqueira J.F.Jr., Rjcas I.N., Oliveira J.C. and Santos K.R.: Molecular detection of black-pigmented bacteria in infections of endodontic origin. J Endod. 27:563-566, 2001 https://doi.org/10.1097/00004770-200109000-00002
  45. Machado de Oliveira J.C ., Siqueira J.F.Jr., Alves G.B., Hirata R.Jr. and Andrade A.F.: Detection of Porphyromonas endodontalis in infected root canals by16S rRNA gene-directed polymerase chain reaction. J Endod, 26:729-732, 2000 https://doi.org/10.1097/00004770-200012000-00016
  46. Slots J.: Selective medium for isolation of Actinobacillus actinomycetemcomitans. J Clin Microbiol, 15:606-609, 1982
  47. Grenier D.: Demonstration of a bimodal coaggregation reaction between Porphyromonas gingivalis and Treponema denticola. Oral Microbiol Immunol, 7: 280-284, 1992 https://doi.org/10.1111/j.1399-302X.1992.tb00589.x
  48. Grenier D.: Nutritional interactions between two suspected periodontopathogens, Treponema denticola and Porphyromonas gingivalis. Infect Immun, 60:5298-5301, 1992
  49. Simonson L.G., McMahon K.T., Childers D.W. and Morton H.E.: Bacterial synergy of Treponema denticola and Porphyromonas gingivalis in a multinational population. Oral Microbiol Immunol, 7:111-112, 1992 https://doi.org/10.1111/j.1399-302X.1992.tb00519.x
  50. Loesche W., J. Bretz W.A., Kerschensteiner D., Stol lJ., Socransky S.S., Hujoel P. and Lopatin D.E.: Development of a diagnostic test for anaerobic periodontal infections based on plaque hydrolysis of benzoyl-DL-arginine-naphthylamide. J Clin Microbial, 28:1551-1559, 1990
  51. Kesavalu L., Holt S.C. and Ebersole J.L.: Virulence of a polymicrobic Treponema denticola and Porphyromonas gingivalis, in a murine model. Oral Microbiol Immunol, 13:373-373, 1998 https://doi.org/10.1111/j.1399-302X.1998.tb00694.x
  52. Loesche W.J. and Grossman N.S.: Periodontal disease as a specific. albeit chronic, infection: diagnosis and treatment. Clin Microbiol Rev. 14:727-752, 2001 https://doi.org/10.1128/CMR.14.4.727-752.2001

피인용 문헌

  1. Microbial profile of asymptomatic and symptomatic teeth with primary endodontic infections by pyrosequencing vol.36, pp.6, 2011, https://doi.org/10.5395/JKACD.2011.36.6.498
  2. Isolation of Propionibacterium acnes among the microbiota of primary endodontic infections with and without intraoral communication vol.20, pp.8, 2016, https://doi.org/10.1007/s00784-016-1739-x