Restorative Dentistry and Endodontics

  • 제28권4호
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  • Pages.295-302
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  • 2003
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  • 2234-7658(pISSN)
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  • 2234-7666(eISSN)
대한치과보존학회 (The Korean Academy of Conservative Dentistry)
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인산 칼슘 근관 봉함재의 세포독성에 관한 연구

Evaluation of the Cytotoxicity of Calcium Phosphate Root Canal Sealers

  • 이지현 (서울대학교 대학원 치의학과 치과보존학교실) ;
  • 백승호 (서울대학교 대학원 치의학과 치과보존학교실) ;
  • 배광식 (서울대학교 대학원 치의학과 치과보존학교실)
  • Lee, Jee-Hyun (Department of Conservative Dentistry, College of Dentistry, Seoul National University) ;
  • Baek, Seung-Ho (Department of Conservative Dentistry, College of Dentistry, Seoul National University) ;
  • Bae, Kwang-Shik (Department of Conservative Dentistry, College of Dentistry, Seoul National University)
  • 발행 : 2003.07.01
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초록

본 연구에서는 인산 칼슘 근관 봉함재 [Apatite Root Sealer (ARS) Type I, II, III]의 세포독성을 혼합후의 시간 경과에 따라서 다른 4계열의 근관 봉함재 (Pulp Canal Sealer EWT, AH Plus, Sealapex, Ketac Endo)와 비교하여 평가하였다. 근관 봉함재를 혼합한 후 1시간, 8시간, 24시간, 48시간, 1주, 2주,4주의 기간동안 배양액을 이용하여 추출액을 얻었다. L929 쥐섬유아세포를 24시간동안 각 시간군에서 얻은 추출액과 함께 배양한 후 dimethylthiazol diphenyltetrazolium (MTT) assay와 neutral red (NR) assay를 이용하여 세포독성(%)을 평가하였다. 실험 결과 ARS Type I, II, III는 전 시간군에 걸쳐 낮은 세포독성을 보였고 (23.65-0.55%) 특히 경화 초기에 다른 계열의 근관 봉함재보다 유의성 있게 낮은 세포독성을 나타내었다. ARS Type I, II, III간의 세포독성은 각 시간군에서 유의성 있는 차이를 보이지 않았다. AH Plus와 Ketac Endo는 초기에 높은 세포독성을 보였으나 AH Plus는 8시간 이후에, Ketac Endo는 24시간 이후에 독성이 급격히 감소하여 ARS와 유의성 있는 차이를 보이지 않았다. Pulp Canal Sealer EWT와 Sealapex는 4주까지 지속적인 세포독성을 나타내었다. 그리고 MTT assay와 NR assay를 이용하여 얻은 각 근관봉함재의 세포독성은 시간 경과에 따라서 비슷한 변화 양상을 나타내었다. 인산 칼슘 근관 봉함재는 생체적합성이 우수한 재료로써 앞으로 지속적인 개발과 평가가 필요할 것으로 사료된다.

키워드

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  2. cytotoxicity of four calcium silicate-based endodontic cements on human monocytes, a colorimetric MTT assay vol.39, pp.3, 2014, https://doi.org/10.5395/rde.2014.39.3.149