한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제32권6호
  • /
  • Pages.931-936
  • /
  • 2003
  • /
  • 1226-3311(pISSN)
  • /
  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
권호 표지
DOI QR코드

DOI QR Code

노루궁뎅이 버섯 추출물이 암세포의 성장과 세포주기 조절단백질에 미치는 영향

Effect of Hericium erinaceus Extract on Cancer Cell Growth and Expression of Cell Cycle Associated Proteins

  • 박선희 (영남대학교 식품영양학과) ;
  • 장종선 (영남대학교 식품영양학과) ;
  • 이갑랑 (영남대학교 식품영양학과)
  • 발행 : 2003.08.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 항암효과를 가진 성분을 포함하여 여러 다양한 생리활성 물질을 함유하고 있을 것으로 기대되는 노루궁뎅이 버섯(Hericium erinaceus)을 이용하여 암세포 성장저해효과와 세포주기 조절자인 cyclin 단백질의 발현에 미치는 영향을 조사하였다. 노루궁뎅이 버섯의 메탄을 추출물과 그 분획물들의 사람의 암세포주인 HT29와 HepG2에 대한 성장 저해 효과를 MTT assay로 검토한 결과 노루궁뎅이 버섯의 메탄을 추출물과 헥산, 클로로포름 그리고 에틸아세테이트 분획물들이 높은 암세포 성장 저해 효과를 나타내었으며 농도 의존적인 경향을 보였다. 그러나 사람의 정상 간세포인 Chang cell에서는 세포독성이 나타나지 않았다 그리고 노루궁뎅이 버섯 메탄올추출물이 간암 세포주인 HepG2의 cyclin 단백질 발현에 미치는 영향을 조사한 결과 노루궁뎅이 버섯 메탄을 추출물이 cyclin A와 D 단백질 발현을 다소 감소시켰으며 특히 cyclin B1에 대한 효과가 더욱 크게 나타나 1 mg/mL 농도에서 48시간 처리 하였을 때 대조군에 비해 30% 정도까지 단백질 발현이 감소하였다. 이러한 결과로 노루궁뎅이 버섯은 세포주기 중 G2기에서 M기로의 전환에 관여하는 cyclin B1 단백질의 발현을 크게 감소시키므로 세포주기 진행을 차단시켜 간암세포의 증식을 억제시키는 것으로 사료된다.

We investigated inhibitory effects of Hericium erinaceus on the growth of cancer cells and the expression of cell cycle regulators, cyclins. Anticancer effects of Hericium erinaceus extract and fractions against cancer cell lines including HepG2 and HT29 were investigated. The methanol extract, the hexane fraction, the chloroform fraction and the ethylacetate fraction of Hericiu erinacew inhibited growth of cancer cells but they had no effect on the cytotoxicity of normal human liver cells under the same conditions. As shown by western blot analysis, the expression of cyclin B1 known as cell cycle regulator was markedly decreased after treatment with Hericium erinaceus extract in HepG2 cells. These results suggest that antiproliferative effect of Hericaum erinaceus extract is associated with markedly decreased expression of cyclin B1.

키워드

참고문헌

  1. Hunter T. 1993. Braking the cycle. Cell 75: 839-841. https://doi.org/10.1016/0092-8674(93)90528-X
  2. Marx J. 1994. How cells cycle toward cancer. Science 263: 319-321. https://doi.org/10.1126/science.8278804
  3. Norbury C, Nurse P. 1992. Animal cell cycles and their control. Ann Rev Biochem 61: 441-444. https://doi.org/10.1146/annurev.bi.61.070192.002301
  4. Pines J, Hunter T. 1990. Human cyclin A is adenovirus E1A associated protein p60 and behaves differently from cyclin B. Nature 346: 760-764. https://doi.org/10.1038/346760a0
  5. Pagano M, Pepperkok R, Verde F, Ansorge W, Draetta G. 1992. Cyclin A is required at two points in the human cell cycle. EMBO J 11: 961-965.
  6. Xiong Y, Connolly T, Futcher B, Beach D. 1991. Human D type cyclin. Cell 65: 691-694. https://doi.org/10.1016/0092-8674(91)90100-D
  7. Lee JH, Choi KS, Kim MY. 1997. Effect of ceramide on cell growth and cell cycle related proteins in U-937 cells. Yakhak Hoeji 41: 94-98.
  8. Jose M, Coco M, Astrid B, Tom V, Francois L, Rob M. 1999. Cyclin D1 overexpression enhances radiation induced apoptosis and radiosensitivity in a breast tumor cell line. Cancer Research 59: 1134-1138.
  9. John MP. 1997. Plant derived anticancer agents. Biochemical Pharmacology 53: 121-124 https://doi.org/10.1016/S0006-2952(96)00654-5
  10. Ahn DK. 1992. Medicinal fungi in Korea. Kor J Mycol 20: 154-166.
  11. Kawagishi H, Shimada A, Hosokawa S, Mori H, Sakamoto H, Ishiguro Y, Sakemi S, Bordner J, Kojima N, Furukawa S. 1996. Erinacines E, F and G stimulators of nerve growth factor (NGF)-synthesis from the mycelia of Hericium erinaceum. Tetrahedron Letters 37: 7399-7402. https://doi.org/10.1016/0040-4039(96)01687-5
  12. Mizuno T. 1995. Yamabushitake, Hericium erinaceum; bioactive substances and medicinal utilization. Food Reviews International 11: 173-178. https://doi.org/10.1080/87559129509541027
  13. Mizuno T, Wasa T, Ito H, Suzuki C, Ukai N. 1992. Antitumor-active polysaccharides isolated from the fruiting body of Hericium erinaceum; an edible and medicinal mushroom called yamabushitake or houtou. Biosci Biotech Biochem 56: 347-348. https://doi.org/10.1271/bbb.56.347
  14. Park SH, Kim OM, Lee KR. 2001. Antimutagenic and quinone reductase inducing activities of Hericium erinaceus extracts. J Kor Soc Food Sci Nutr 30: 1287-1292.
  15. Green LM, Reade JL, Ware CF. 1984. Rapid colometric assay for cell viability: Application to the quantitation of cytotoxic and growth inhibitory lymphokines. J Immunological Methods 70: 257-263. https://doi.org/10.1016/0022-1759(84)90190-X
  16. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analyt Biochem 72: 248-268. https://doi.org/10.1016/0003-2697(76)90527-3
  17. Chang JS. 2002. Study on the mechanism of cancer chemoprevention of Hypsizigus marmoreus. PhD Dissertation. Yeungnam University.
  18. Kim SH, Park KY. 1993. Inhibitory effects of chinese pepper on the mutagenicity and the growth of MG-63 human osteosarcoma cells. Kor J Appl Microbiol Biotechnol 21: 628-634.
  19. Park KY, Kim SH, Suh MJ, Chung HY. 1991. Effects of Garlic on the mutagenicity in Salmonella assay system and on the growth of HT-29 human colon carcinoma cells. Kor J Food Sci Technol 23: 370-374.
  20. Choi SY, Cheigh MJ, Lee BK. 1999. Growth suppression effect of traditional fermented soybean paste on the various tumor cells. J Kor Soc Food Sci Nutr 28: 458-463.
  21. Annette JH, Christopher AS, Leslie CS, Victoria EAS, Vanessa LL, Aedin CC, Gwyn TW. 1996. Apoptosis, molecular regulation of cell death. Eur J Biochem 236: 1-26. https://doi.org/10.1111/j.1432-1033.1996.00001.x
  22. Bae JT, Chang JS, Lee KR. 2002. Effect of Sarcodon aspratus extract on expression of cell cycle associated proteins in HepG2 cells. J Kor Soc Food Sci Nutr 31: 329-332. https://doi.org/10.3746/jkfn.2002.31.2.329
  23. Ahmad N, Cheng P, Mukhtar H. 2000. Cell cycle deregulation by green tea polyphenol epigallocatechin-3-gallate. Biochem Biophys Res Commum 275: 328-334. https://doi.org/10.1006/bbrc.2000.3297
  24. Lim CC, Kim IH, Cho JW, Beak WK, Sohn SS. 2000. Expression of cell cycle control genes in Korean gastric cancer cell lines. J Korean Cancer Assoc 32: 279-287.

피인용 문헌

  1. Physicochemical Characteristics and Antioxidant activity, Antimutagenicity, and Cytotoxicity of Hot-water Extract of Hericium erinaceus vol.28, pp.5, 2012, https://doi.org/10.9724/kfcs.2012.28.5.569
  2. 인진쑥 노루궁뎅이 버섯균사체 발효물의 항산화 활성 vol.31, pp.4, 2014, https://doi.org/10.12925/jkocs.2014.31.4.719
  3. 신경세사 단백질의 산화적 손상에 엄나무 발효물이 미치는 영향 vol.35, pp.1, 2018, https://doi.org/10.12925/jkocs.2018.35.1.194
  4. 엄나무 발효물의 항산화 및 항아밀로이드 활성 vol.35, pp.2, 2003, https://doi.org/10.12925/jkocs.2018.35.2.389