Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Isolation of protoplast from the marine red alga Porphyra pseudolinearis in Korea

한국산 해산 홍조류 긴잎돌김(Porphyra pseudolinearis Ueda)에서의 원형질체 분리

  • Kim, Young-Dae (Aquaculture Division, East Sea Regional Fisheries Research Institute) ;
  • Choi, Jae-Seok (Department of Biotechnology, Pukyong National University) ;
  • Lee, Ju (Aquaculture Division, East Sea Regional Fisheries Research Institute) ;
  • Son, Yong-Soo (Aquaculture Division, East Sea Regional Fisheries Research Institute) ;
  • Hong, Yong-Ki (Department of Biotechnology, Pukyong National University)
  • 김영대 (국립수산과학원 동해수산연구소 증식과) ;
  • 최재석 (부경대학교 생물공학과) ;
  • 이주 (국립수산과학원 동해수산연구소 증식과) ;
  • 손용수 (국립수산과학원 동해수산연구소 증식과) ;
  • 홍용기 (부경대학교 생물공학과)
  • Published : 2003.08.01
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Abstract

Optimal conditions of protoplast isolation from the seaweed Prophyra pseudolinearis have been described. The P. pseudolinearis is one of indigenous and dominant Porphyra species in the East Sea region. Protoplasts have been released by enzymatic treatment of 2% agarase and 2% hemicellulase in 25mM MES buffer, pH 6.0 containing 0.5 M sorbitol. The protoplasts could be fused with neutral red-stained protoplasts of P. okamurae by the addition of polyethylene glycol 8000 solution.

한국 동해안 특산종인 긴잎돌김의 원형질체 분리를 위한 적정한 조건을 구명하기 위한 연구가 이루어 졌다. 홍조류 긴잎돌김은 동해안에 있어 토착종이며 우점종중 하나이다. 원형질체는 2% agarase and 2% hemicellulase 의 효소를 이용하여 25mM Mes buffer, pH 6.0의 0.5M 솔비톨에서 분리되어 졌다. 분리되어진 원형질체는 PEG 8000에서 염색한 오카무라둥근돌김과 접합하였다.

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References

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