DOI QR코드

DOI QR Code

A Study on the Cultural Characteristics of Pholiota nameko Mycelium

맛버섯 균사체의 배양 특성에 관한 연구

  • Published : 2003.08.01

Abstract

This Study was carried out to investigate the optimal mycelial growth of Pholiota nameko. The optimal medium for the mycelial growth was ME medium. The optimal temperature and pH were $25^{\circ}C$$\pm$1 and 5.5, respectively. The modified optimal medium compositions were glucose 3% (w/v), malt extract 0.25% (w/v), yeast extract 0.25% (w/v), $KH_2PO_4$ 0.046% (w/v), $K_2HPO_4$ 0.1% (w/v), $MgSO_4$$7H_2O$ 0.05% (w/v). From the result of experiments on the optimal temperature, pH and nutritional requirements, the mycelial growth of modified optimal medium was higher than that of ME medium.

맛버섯( Pholiota nameko KACC50453)의 균사체 배양 특성에 관한 연구 결과 맛버섯 균사체의 배양에 적합한 기본 배지는 ME였으며, 최적 배양 온도는 $25^{\circ}C$, 최적 초기 pH는 5.5였다. 맛버섯 균사체 생장을 위한 최적 배지 조성은 glucose 3%(w/v),malt extrac 0.25%(w/v), yeast extract 0.25%(w/v),$KH_2PO_4$ 0.046%(w/v),$K_2HPO_4$ 0.1%(w/v),$MgSO_4$ $.$$7H_2O$0.05% (w/v)로 나타났다.기본배지(ME)와 최적배지로 액체 배양한 결과 최적 배지에서 균사생장이 양호하였다.

Keywords

References

  1. Chang, H. Y., D. Y. Cha, A. S. Kang, I. P. Hong, K. P. Kim, S. J. Seok, Y. J. Ryu and J. M. Sung. 1995. Cultural characteristics of Fomitella fraxinea (Fr.) Imaz. Kor. J. Mycol. 23, 238-245.
  2. Chang, S. T. and W. A. Hayes. 1978. The biology and cultivation of edible mushrooms. pp. 461-473, Academic Press.
  3. Fuji, T., H. Maeda and N. Ishida. 1978. Isolation and characterization of a new antitumor polysaccharide, KS-2, extracted from culture mycelia of Lentinus edodes. J. Antibiot. 31, 1079-1090. https://doi.org/10.7164/antibiotics.31.1079
  4. Go, S. J., C. H. You and D. Y. Cha. 1981. Studies on the artificial substrate with rice straw and the spawning for the Pleurotus florida in Korea. Kor. J. Mycol. 9, 67-72.
  5. Hashimoto, K. and Z. Takahasi. 1974. Studies on the growth of Pleurotus ostreatus. Mushroom Science 9, 585-593.
  6. Jung, I. C., S. H. Kim, Y. I. Kwon, S. Y. Kim, J. S. Lee, S. Park, K. S. Park and J. S. Lee. 1997. Cultural condition for the mycelial growth of Phelinus igniarius on chemically defined medium and grains. Kor. J. Mycol. 25, 133-142.
  7. Kawai, M. and S. Abe. 1976. Studies on the artificial reproduction of Tricholoma matsutake (S. Ito et Imai) Sing. I. Effect of carbon and nitrogen sources in media on the vegetative growth of Tricholoma matsutake. Trans. Mycol. Soc. Japan 17, 159.
  8. Kazun, C., M. Nishijima, H. Miura and I. Kamoi. 1990. Structural examination of water-soluble glycans from fruit body of Lyophyllum ulmarium. Nippon Snokuhin Kogyo Gakkaishi 37, 765-772. https://doi.org/10.3136/nskkk1962.37.10_765
  9. Kim, B. K., M. J. Shin, O. N. Kim, H. W. Kim and E. C. Choi. 1989. Antitumor components of the cultural of Lepiota procera. Kor. J. Food Hygiene. 4, 109-118.
  10. Lee. J. W., C. H. Chung, H. J. Jeong and K. H. Lee. 1990. Anticomplementory and antitumor activities of the IKI, extract form the mycelia of Lentinus edodes IY-105. Kor. J. Appl. Microbiol. Biotechnol. 18, 571-577.
  11. Lee, J. Y. 1982. Mycology and cultivation of mushrooms. pp. 339-341, 3th eds., Daekwang Munhwasa, Seoul, Korea.
  12. Mizuno, T., K. Oshawa, N. Hagiware and R. Kuboyama. 1986. Fractionation and characterization of antitumor polysaccharides from Maitake, Grifola frondosa. Agric. Biol. Chem. 50, 1679-1688. https://doi.org/10.1271/bbb1961.50.1679
  13. Park, Y. D., Y. K. Hong, W. K. whang, J. D. Huh and S. Park. 1989. Comparisons of protein-bound polysaccharide contents obtained from mycelia culture broth and fruit body of Coriolus versicolor. Kor. J. Appl. Mycol. 17, 223-228.
  14. Usui, I., Y. Iwasaki and T. Mizuno. 1983. Isolation and characterization of antitumor $\beta$-D-glucans the fruit body of Garnoderma applanatum. Carbohydr. Res. 115, 273-280. https://doi.org/10.1016/0008-6215(83)88159-2
  15. Wolport, F. S. 1924. Studies on the physiology of fungi. ⅩⅦ. The growth of certain wood-destroying fungi in relation to the H-ion concentration of the media. Ann. Misouri Bot. Garden, 11-97.
  16. Yoo, K. H., J. H. Kim and S. J. Seok. 2001. Studies on the cultural charateristics of Hohenbuehelia petaloides. Kor. J. Mycol. 29, 52-60.
  17. Zadrazil, F. 1974. The ecology and industrial production of Pleurotus ostreatus, Pleurotus florida, Pleurotus cormucopiae and Pleurotus eryngii. Mushroom Science 9, 621-655.

Cited by

  1. Homology search of genus Pleurotus using an internal transcribed spacer region vol.24, pp.3, 2007, https://doi.org/10.1007/s11814-007-0070-2
  2. Comparative evaluation of antioxidant, nitrite scavenging, and antitumor effects of Antrodia camphorata extract vol.14, pp.2, 2009, https://doi.org/10.1007/s12257-008-0291-1
  3. Effects ofFomitopsis pinicola extracts on antioxidant and antitumor activities vol.12, pp.5, 2007, https://doi.org/10.1007/BF02931349
  4. Antioxidant and Anti-Inflammatory Activities of Methanol and Hot Water Extracts of Pholiota nameko Fruiting Bodies vol.41, pp.2, 2013, https://doi.org/10.4489/KJM.2013.41.2.97