생명과학회지 (Journal of Life Science)

  • 제13권4호
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  • Pages.416-420
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  • 2003
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Microsatellite DNA형에 의한 개의 친자감정예

A Case of Parentage Testing in Dog by Microsatellite DNA Typing

  • 조길재 (한국마사회 유전자검사실) ;
  • 조병욱 (밀양대학교 동물자원학과) ;
  • 이길왕 (밀양대학교 동물자원학과) ;
  • 김선구 (밀양대학교 동물자원학과) ;
  • 김용균 (밀양대학교 생물공학과)
  • 발행 : 2003.08.01
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초록

Microsatellite DNA형에 의한 개의 친자감정을 실시한 결과 다음과 같은 결론을 얻었다. Labrador Retriever Pup I과 Pup II는 12개 marker 모두에서 멘델의 유전법칙에 따라 친자관계가 성립되었으나 풍산개인 Pup III은 PEZ1 (106bp/118bp), PEZ10 (276bp/300bp), FHC2010 (228bp/232bp) 등 3개 marker에서 유전법칙에 어긋나 친자관계가 성립되지 않았다.

We performed this study to determine the parentage verification of putative dogs. A total of 7 samples (4 Labrador Retriever dog and 3 Poongsan dog) were genotyped by using 12 international markers (PEZ1, PEZ5, PEZ8, PEZ10, PEZ11, PEZ12, PEZ17, PEZ20, PEZ21, FHC2010, FHC2054, FHC2079). This methods consisted of multiplexing PCR procedures, and it showed reasonable amplification of all PCR products. Genotyping was performed with an ABI 310 genetic analyzer. Labrador Retriever Pup I and Pup II were included according to principles of Mendelian genetics in all loci, while Poongsan Pup III was excluded with markers PEZ1 (106/118), PEZ10 (276/300), and FHC2010 (228/232). These results suggest that the present DNA typing is so useful for parentage verification of these two breeds.

키워드

참고문헌

  1. Cho, G. J. 2002. Microsatellite DNA polymorphism of thoroughbred horses in Korea. Korean J. Genetics 24, 177-182.
  2. Cho, G. J., Y. J. Yang, H. S. Kang and B. W. Cho. 2002. Genetic diversity and validation of microsatellite markers for Jeju native horse parentage testing. Korean J. Genetics 24, 359-365.
  3. Fredholm, M. and A. K. Wintero. 1995. Efficient resolution of parentage in dogs by amplification of microsatellites. Animal Genetics 27, 19-23.
  4. Glowatzki-Mullis, M. L., C. Gaillard, G. Wigger and R. Feies. 1995. Microsatellite-based parentage control in cattle. Animal Genetics 26, 7-12. https://doi.org/10.1111/j.1365-2052.1995.tb02612.x
  5. Halverson, J. L. and J. W. Edwards. 2000. Microsatellite polymorphism in dog breeds-the AKC Parent Club study. Proc. 27th ISAG Conference on Animal Genetics. pp. 19.
  6. Han, S. K., H. D. Byun and E. Y. Chung. 2000. Genetic diversity analysis and parentage control in Korean native cattle using Microsatellite. Proc. 27th ISAG Conference on Animal Genetics. pp. 88.
  7. Jeffreys A. J., V. Wilson and S. L. Thein. 1985. Hypervariable minisatellite regions in human DNA. Nature 314, 67-73. https://doi.org/10.1038/314067a0
  8. Kim, K. S. and C. B. Choi. 2002. Genetic structure of Korean native pig using microsatellite markers. Korean J. Genetics 24, 1-7.
  9. Litt, M. and J. A. Luty. 1989. A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. American J. Human Genetics 44, 397-401.
  10. Tozaki, T., H. Kakoi, S. Mashima, K. I. Hirota, T. Hasegawa, N. Ishida, N. Miura, N. H. Choi-Miura and M. Tomita. 2001. Population study and validation of paternity testing for thoroughbred horses by 15 microsatellite loci. J. Veterinary Medicine Science 63, 1191-1197. https://doi.org/10.1292/jvms.63.1191