Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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A Case of Parentage Testing in Dog by Microsatellite DNA Typing

Microsatellite DNA형에 의한 개의 친자감정예

  • 조길재 (한국마사회 유전자검사실) ;
  • 조병욱 (밀양대학교 동물자원학과) ;
  • 이길왕 (밀양대학교 동물자원학과) ;
  • 김선구 (밀양대학교 동물자원학과) ;
  • 김용균 (밀양대학교 생물공학과)
  • Published : 2003.08.01
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Abstract

We performed this study to determine the parentage verification of putative dogs. A total of 7 samples (4 Labrador Retriever dog and 3 Poongsan dog) were genotyped by using 12 international markers (PEZ1, PEZ5, PEZ8, PEZ10, PEZ11, PEZ12, PEZ17, PEZ20, PEZ21, FHC2010, FHC2054, FHC2079). This methods consisted of multiplexing PCR procedures, and it showed reasonable amplification of all PCR products. Genotyping was performed with an ABI 310 genetic analyzer. Labrador Retriever Pup I and Pup II were included according to principles of Mendelian genetics in all loci, while Poongsan Pup III was excluded with markers PEZ1 (106/118), PEZ10 (276/300), and FHC2010 (228/232). These results suggest that the present DNA typing is so useful for parentage verification of these two breeds.

Microsatellite DNA형에 의한 개의 친자감정을 실시한 결과 다음과 같은 결론을 얻었다. Labrador Retriever Pup I과 Pup II는 12개 marker 모두에서 멘델의 유전법칙에 따라 친자관계가 성립되었으나 풍산개인 Pup III은 PEZ1 (106bp/118bp), PEZ10 (276bp/300bp), FHC2010 (228bp/232bp) 등 3개 marker에서 유전법칙에 어긋나 친자관계가 성립되지 않았다.

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References

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