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Detection of Silent Allele at Esterase(Es) Locus in Jeju Native Horse

제주마에서 Esterase(Es) locus의 silent allele 검출

  • 조길재 (한국마사회 유전자검사실) ;
  • 조병욱 (밀양대학교 말과학연구소) ;
  • 강한석 (밀양대학교 말과학연구소) ;
  • 김용균 (밀양대학교 생물공학과)
  • Published : 2003.08.01

Abstract

The purpose of this present study was to investigate the polymorphism of esterase locus for individual identification and parentage verification in Jeju native horse (JNH). Seventy three random JNH samples were studied by polyacrylamide gel isoelectric focusing(IEF) at pH 3.5 ∼ 6.0. We detected international recognized alleles, F, G, H, I, M, and an silent allele $I^o$. Gene frequencies of allele I showed 0.479 the highest, while allele H and M($I^o$) with relatively low frequencies were 0.027 and 0.014, respectively.

제주마의 Es유전적 다형은 F, G, H, I, M, $I^o$ 의 6개의 대립유전자가 분포되어 있으며, 대립유전자 II가 22두(30.1%), FI 16두(21.9%), FF 9두(12.3%), GI 9두(12.4%) 순으로 높은 분포를 보였다. 또한 특이적인 silent 대립유전자로 추정되는 $I^oI^o$가 1두(1.4%)에서 관찰되었다. Es의 유전자 빈도는 대립유전자 I가 47.9%로 가장 높은 빈도를 보였으며 그 다음은 F (27.4%), G (19.2%), H (2.7%), M과 $I^o$가 각각 1.4% 순으로 분포하였다.

Keywords

References

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