Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Identification of Quantitative Trait Loci for Resistance to Soybean Cyst Nematode Race 14

콩시스트 선충 race14에 대한 저항성 유전자좌 구명

  • Published : 2003.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

A direct and precise explanation of soybean resistance to soybean cyst nematode will be possible only when the individual gene(s) involved in the resistance are tagged. This study was conducted, (1) to identify and localize quantitative trait loci for resistance to soybean cyst nematode race 14 on RAPD map, (2) to identify the magnitude and mode of inheritance for each quantitative trait loci, and (3) to identify the best combinations of quantitative trait loci for resistance to soybean cyst nematode race 14. Thirty markers (29 RAPD and 1 RFLP) showed significant association with resistance to soybean cyst nematode race 14. From MAPMAKER/QTL analysis, we identified two regions (linkage group C-7 and linkage group C-9) for resistance to soybean cyst nematode .ace 14. The first quantitative trait loci that was localized at 6.0 cM from $H06^1$ on linkage group C-7 showed a dominant inheritance mode. However, we can not exclude the possibility of additive inheritance mode. The second quantitative trait loci that was localized between $B15^2$ and $E01^1$ on linkage group C-9 also showed a dominant mode of inheritance. One pair of flanking markers ($H06^1$ and $H06^2$) and B15$^2$ were used for multiple regression analysis. Marker combination that included 2 markers, $B15^2$ and $H06^1$, explained the highest total variance (22.9%) for resistance to soybean cyst nematode race 14. Further localization of genes for resistance to soybean cyst nematode race 14 and examination of interaction between quantitative trait loci will accelerate the exploitation of resistance to soybean cyst nematode.

본 연구는 콩 cyst 선충 race 14에 대한 저항성 QTLs 구명을 목적으로 한 바 결과를 요약하면 다음과 같다. 1. 회귀분석 결과 30개의 marker들(29 RAPD, 1 RFLP)에서 cyst 선충 race 14의 저항성에 대한 유의성이 인정되었다. 2. MAPMAKER/QTL 분석 결과 2개의 QTL들이 구명되었는데, 이 QTL들은 2개의 linkage groups (LGC-7와 LGC-9)에 위치하였으며, 모두 우성유전 양상을 나타내었다. 3. 다중회귀분석 결과 2개의 marker들($B15^2$와 $H06^1$)로 구성된 조합에서 가장 높은 표현적 변이의 값(22.9%)을 나타내었다. 콩 cyst 선충 rare 14에 대한 표현적 변이를 충분히 설명하기 위해서는 지속적인 QTL 구명 연구가 요구된다.

Keywords

References

  1. Anand, S. C. 1991. Registration of soybean germplasm line S88-2036 having multiple-race soybean cyst nematode resistance. Crop Sci. 31, 856. https://doi.org/10.2135/cropsci1991.0011183X003100030077x
  2. Anand, S. C., A. J. Wrather and C. R. Shumway 1985. Soybean genotypes with resistance to races of soybean cyst nematode. Crop Sci. 25, 1073-1075. https://doi.org/10.2135/cropsci1985.0011183X002500060042x
  3. Boutin, S, Ansari, V. Concibido, R. Denny, J. Orf and N. Young 1992. RFLP analysis of cyst nematode resistance in soybeans. Soybean Genet. Newsl. 19, 123-127.
  4. Caldwell, B. E., C. A. Brim and J. P. Ross 1960. Inheritance of resistance in soybeans to the cyst nematode Heterodera glycines. Agronomy J. 52, 635-636. https://doi.org/10.2134/agronj1960.00021962005200110007x
  5. Choi, I. S. and H. T. Skorupska 1995. RAPD marker selection using DNA composite of cultivars for differential response to soybean cyst nematode races 3, 5, and 14. Soybean Genetics Newsl. 250, 245-250.
  6. Choi, I. S. and H. T. Skorupska 1997a. Random Amplified Polymorphic DNA map for intraspecific cross of Essex x PI 437654 in Glycine max(L.) Merr. Korean J. Breeding 29, 270-281.
  7. Choi, I. S. and H. T. Skorupska 1997b. Identification of Quantitative Trait Loci for Resistance to Soybean Cyst Nematode Race 3 in Soybean. Korean J. Breeding 29, 166-177.
  8. Concibido, V. C., R. L. Denny, S. R. Boutin, R. Hautea, J. H. Orf and N. D. Young, 1994. DNA marker analysis of loci underlying resistance to soybean cyst nematode (Heterodera glycines Ichinohe). Crop Sci. 34, 240-246. https://doi.org/10.2135/cropsci1994.0011183X003400010044x
  9. Lincoln, S., M. Daly and E. Lander 1992. Mapping genes controlling quantitative traits with MAPMAKER/QTL 1.1. Whitehead Institute Technical Report. 2nd edition.
  10. Luedders, V. D. 1989. Inheritance of genes for resistance to soybean cyst nematode populations. Crop Sci. 29, 667-671. https://doi.org/10.2135/cropsci1989.0011183X002900030025x
  11. Mahalingam, R. and H. T. Skorupska 1995. DNA markers for resistance to Heterodera glycines I. race 3 in soybean cultivar Peking. Plant Breeding 45, 435-443.
  12. Matson, A. L. and L. F. Williams 1965. Evidence of a fourth gene for resistance to the soybean cyst nematode. Crop Sci. 5, 477. https://doi.org/10.2135/cropsci1965.0011183X000500050032x
  13. Myers, G. O. and S. C. Anand 1991. Inheritance of resistance and genetic relationships among soybean plant introductions to races of soybean cyst nematode. Euphytica 55, 197-201. https://doi.org/10.1007/BF00021239
  14. Paterson, A., E. Lander, S. Lincoln, J. Hewitt, S. Paterson and S. Tanksley 1988. Resolution of Quantitative traits into Mendelian factors using a complete RFLP linkage map. Nature 335, 721-726. https://doi.org/10.1038/335721a0
  15. Rao-Arelli, A. P. and S. C. Anand 1988. Genetic relationships among soybean plant introductions for resistance to race 3 SCN. Crop Sci. 28, 650-652. https://doi.org/10.2135/cropsci1988.0011183X002800040017x
  16. Rao-Arelli, A. P., S. C. Anand and J. A. Wrather 1992. Soybean resistance to soybean cyst nematode race 3 is conditioned by an additional dominant gene. Crop Sci. 32, 862-864. https://doi.org/10.2135/cropsci1992.0011183X003200040005x
  17. Riggs, R. D. and D. P. Schmitt. 1987. Nematodes. pp.757-778. In: Soybeans: Improvement, Production, and Uses. J. R. Wilcox (ed.) American Society of Agronomy, Inc., Madison, Wisconsin, USA.
  18. SAS 1990. SAS/STAT User's Guide, version 6.0. 3rd ed. SAS Institute Inc. SAS campus Drive, Cary, NC.
  19. Skorupska, H. T., A. Warner and I. S. Choi, 1995. Identification of PCR-generated DNA variation in transmission analysis with PI 437654. Soybean Genetics Newsl. 250, 240-244.
  20. Soller, M. and J. Poltkin-Hazan 1977. The use of marker alleles for the introgressions of linked quantitative alleles. Genetics 51, 133-137.
  21. Sugiyama, S. and H. Katsumi 1966. A resistant gene to the soybean cyst nematode observed from cross between Peking and Japanese varieties. Jpn. J. Breeding 16, 83-86. https://doi.org/10.1270/jsbbs1951.16.83
  22. Weisemann, J. M., B. F. Mattews and T. E. Devine 1992. Molecular markers located proximal to the soybean cyst nematode resistance gene, Rhg4. Theor. Appl. Genet. 85, 136-138.