Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Desulfurization of Dibenzothiophene and Diesel Oil by Metabolically Engineered Escherichia coli

  • Park, Si-Jae (Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biocolecular Engineering and BioProcess Engineering Research Center) ;
  • Lee, In-Su (Department of Chemical and Biocolecular Engineering and BioProcess Engineering Research Center) ;
  • Chang, Yong-Keun (Department of Chemical and Biocolecular Engineering and BioProcess Engineering Research Center) ;
  • Lee, Sang-Yup (Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biocolecular Engineering and BioProcess Engineering Research Center, Bioinformatics Research Center, Korea Advanced Institute of Science and Technology)
  • Published : 2003.08.01
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Abstract

The desulfurization genes (dszABC) were cloned from Gordonia nitida. Nucleotide sequences similarity between the dszABC genes of G. nitida and those of Rhodococcus rhodochrous IGTS8 was 89%. The similarities of deduced amino acids between the two were 86% for DszA, 86% for DszB, and 90% for DszC. The G. nitida dszABC genes were expressed in several different Escherichia coli strains under an inducible trc promoter. Cultivation of these metabolically engineered E. coli strains in the presence of 0.2 mM dibenzothiophene (DBT) allowed the conversion of DBT to 2-hydroxybiphenyl (2-HBP), which is the final metabolite of the sulfur-specific desulfurization pathway. The maximum conversion of DBT to 2-HBP was 16% in 60 h. Recombinant E. coli was applied for the deep desulfurization of diesel oil supplemented into the medium at 5% (v/v). Sulfur content in diesel oil was decreased from 250 mg sulfur/1 to 212.5 mg sulfur/1, resulting in the removal of 15% of sulfur in diesel oil in 60 h.

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References

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