Journal of Microbiology and Biotechnology

  • 제13권4호
  • /
  • Pages.544-551
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  • 2003
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Differential Responses of Three Cyanobacteria to UV-B and Cd

  • Atri, Neelam (Laboratory of Algal Biology, Department of Botany, Banaras Hindu University) ;
  • Rai, Lal-Chand (Laboratory of Algal Biology, Department of Botany, Banaras Hindu University)
  • 발행 : 2003.08.01
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초록

Interactive effects of UV-B and Cd on growth, pigment, photosynthesis, and lipid peroxidation have been studied in Anabaena, Microcystis, and Nostoc; all the tested cyanobacteria showed differential sensitivity to different dosage of UV-B and Cd alone as well as in combination. Phycocyanin was severely affected by UV-B and Cd by all the strains; the degree of pigment bleaching was most pronounced in Anabaena followed by Microcystis and Nostoc. $UV-B_2+Cd_2$ produced nearly 83, 78, and 65% inhibition of phycocyanin in Anabaena, Microcystis, and Nostoc, respectively. The above treatment also significantly decreased the contents of Chl ${\alpha}$ and carotenoid. Presence of capsule in Microcystis protected the phycocyanin bleaching as compared to decapsulated cells. Laboratory-grown Microcystis revealed about 75 and 80% inhibition, following $UV-B_2+Cd_2$ treatment, respectively. in capsulated and decapsulated cells. Damage caused by Cd was more pronounced than UV-B. Inhibition of photosynthesis did occur in all the test strains, being maximum in Anabaena. PS II was the most sensitive component of the electron transport chain, showing 84, 80, and 70% inhibition in Anabaena, Microcystis, and Nostoc, respectively. As compared to control, significant lipid peroxidation (6.5-fold higher) was observed in Anabaena with $UV-B_2+Cd_2$, $^{14}C-uptake$ was more susceptible to Cd and Uv-B than oxygen-evolution. Approximately 84, 80, and 76% inhibition of $^{14}C-uptake$ was observed in Anabaena, Microcystis, and Nostoc, respectively. Similarly, $UV-B_2+Cd_2$ inhibited APT content of Anabaena by 87%. This ,study suggests that inhibition of carbon fixation was due to decreased ATP content of the test cyanobacteria by UV-B+Cd, where Anabaena was the most sensitive and Nostoc the most tolerant.

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참고문헌

  1. J. Biol. Chem. v.270 Structure of a novel oligosaccharide-mycosporine-amino acid ultraviolet A/B sunscreen pigment from the terrestrial cyanobacterium Nostoc Commune. Bohm,G.A.;W.Pfleiderer;P.Boger;S.Scherer https://doi.org/10.1074/jbc.270.15.8536
  2. J. Photochem. Photobiol. v.4 Target site of UVB radiation in photosynthesis of higher plants Bornman,J.F. https://doi.org/10.1016/1011-1344(89)80001-6
  3. Biochem. Biophys. Acta v.50 A quantitative assay for the number of chromophores on a chromoprotein. Its application to phycoerytrin and phycocyanin Brody,S.S.;M.Brody https://doi.org/10.1016/0006-3002(61)90333-X
  4. Limnol. Oceanogr. v.38 Response of metallothionein concentrations in a freshwater Anodonta grandis along an environmental cadmium gradient Couillard,Y.P.;G.C.Campbell;A.Tessier https://doi.org/10.4319/lo.1993.38.2.0299
  5. Nature v.356 Ozone depletion-ultraviolet on the increase Crutzen,P.J. https://doi.org/10.1038/356104a0
  6. J. Plant Physiol. v.135 Copper-induced damage to the permeability barrier in roots of Silene cucubalus De Vos;C.H.R.;H.Schat;R.Vooijs;W.H.O.Ernst https://doi.org/10.1016/S0176-1617(89)80171-3
  7. Z. Naturforsch. v.41 Impact of UV-B radiation on photosynthesis, assimilation of 14C-bicarbonate and inorganic 15N-compounds by cyanobacteria Dohler,G.;I.Biermann;J.Zink
  8. Acta Protozool. v.36 Ultraviolet radiation effects on pigmentation in the cyanobacterium Phormidium uncinatum Donkor,V.A.;D.P.Hader
  9. Am. J. Bot. v.27 The isolation, purification and culture of blue-green algae Gerloff,G.C.;G.P.Fitzerald;F.Skoog
  10. Ecol. Prog. Ser. v.114 Effect of UV-B radiation on the fatty acid composition of the marine phytoplankton Tetraselmis sp.:Relationsship to celluar pigments Goes,J.I;N.Handa;S.Taguchi;T.Hama https://doi.org/10.3354/meps114259
  11. Acta Protozool. v.31 UV effects on photosynthetic oxygen production and chromoprotein composition in a freshwater flagellate Cryptomonas Haberlein,A.;D.P.Hader
  12. Plant Cell Physiol. v.27 Inhibition of motility in the cyanobacterium Phormidium uncinatum, by solar and monochromatic UV irradiation Hader,D.P.;M.Watanabe;M.Furuya
  13. Photochem. Photoboil. v.53 Effect of enhanced solar-ultraviolet radiation on aquatic ecosystems Hader,D.P.;R.C.Worrest https://doi.org/10.1111/j.1751-1097.1991.tb08502.x
  14. Protochem. Photobiol. v.52 UV-induced protein alterations and lipid oxidation in erythrocyte membranes Kochevar,I. https://doi.org/10.1111/j.1751-1097.1990.tb08672.x
  15. Physiol. Plant v.58 Comparative study of the action of ultraviolet-C and ultraviolet-B rediation on photosynthetic electron transport Kulandaivelu,G.;A.M.Noorudeen https://doi.org/10.1111/j.1399-3054.1983.tb04199.x
  16. Proc. Natl. Acad. Sci. USA v.93 Ultraviolet-B photodestruction of a light-harvesting complex Lao,K.;A.Glazer https://doi.org/10.1073/pnas.93.11.5258
  17. Plant Cell Physiol. v.22 Firefly assay of adenine nucleotides from algae:Comparison of extraction methods Larson,C.M.;T.Olsson
  18. Phytochemistry v.27 The antioxidants of higher plants Larson,R.A. https://doi.org/10.1016/0031-9422(88)80254-1
  19. Handbook of Phycological Methods: Physiological and Biochemical Methods Hill reaction and phosphorylation with chloroplast preparations from Chlamydomonas reinhardtii Lien,S.
  20. J. Biol. Chem. v.193 Protein measurement with the folin phenol reagent Lowry,O.H.;N.J.Rosebrough;A.L.Farr;R.J.Randall
  21. J. Biol. Chem. v.140 Absorption of light by chlorophyll solutions Mackinney,G.
  22. Physiol. Plant v.94 Oxidative stress and antioxidant content in Chlorella vulgaris after exposure to ultraviolet-B radiation Malanga,G.;S.Punturalo https://doi.org/10.1111/j.1399-3054.1995.tb00983.x
  23. J. Gen. Phycol. v.39 Relationship between pigment content and photosynthetic characterist cs in a blue-green algae Myers,J.;W.A.Kratz
  24. Parker, D. L. 1982. Improved procedures for the cloning and purification of Microcystis cultures (Cyanophyta). J. Phycol. 18: 471-477 https://doi.org/10.1111/j.1529-8817.1982.tb03212.x
  25. J. Phycol. v.18 Improved procedures for the cloning and purification of Microcystis cultures(Cyanophyta) Parker,D.L. https://doi.org/10.1111/j.1529-8817.1982.tb03212.x
  26. Appl. Environ Microbiol. v.64 Effect of cellular metabolism and viability on metal ion accumulation by cultured biomass from a bloom of the cyanobacterium Microcysis aeruginosa Parker,D.L.;L.C.Rai;N.Mallick;P.K.Ra;H.D.Kumar
  27. FEMS Microbiol. Lett. v.82 Metal-induced inhibition of photosynthetic electron transport chain of the cyanobacterium Nostoc muscorum Prasad,S.M.;J.B.Singh;L.C.Rai;H.D.Kumar https://doi.org/10.1111/j.1574-6968.1991.tb04846.x
  28. Environ. Exp. Bot. v.35 Influence of UV-B radiation on membrane lipid composition and ethylene evolution in Doyene dHiver pear shoots grown in vitro under different photosynthetic photon fluxes Predieri,S.;H.A.Norman;D.T.Krizek;P.Pillai;R.M.Mirecki;R.M.Zimmerman https://doi.org/10.1016/0098-8472(95)00003-2
  29. Environmental Health XVI: A symposium The growth response of the green alga Chlorella saccharophilla to selected concentrations of the heavy metals Cd, Cu, Pb, Zn Rachlin,J.W.;T.E.Jensen;B.Warkentine;Hemphill,D.P.(ed.)
  30. J. Gen. Appl. Microbiol. v.31 Effect of nickel and silve ions on survival, growth, carbon fixation and nitrogenase activity in Nostoc muscorum. Regulation of toxicity by EDTA and calcium Rai,L.C.;M.Raizada https://doi.org/10.2323/jgam.31.329
  31. Environ. Exp. Bot. v.35 Interactive effects of UV-B and copper on photosynthetic activity of the cyanobacterium Anabaena doliolum Rai,L.C.;B.Tyagi,N.Mallick;P.K.Rai https://doi.org/10.1016/0098-8472(94)00046-8
  32. Protochem. Photobiol. v.64 Alteration in photosynthetic characteristics of Anabaena doliolum following exposure to UV-B and Pb Rai,L.C.;B.Tyagi;N.Mallick https://doi.org/10.1111/j.1751-1097.1996.tb03120.x
  33. Curr. Sci. v.74 Biotechnological potential of naturally occurring and laboratory-grown Microcystis in bisorption of ${Ni}^{2+}$ and ${Cd}^{2+}$ Rai,L.C.;S.Singh;S.Pardhan
  34. Proceedings of Indo-US Workshop on Impact of Global Climatic Changes in Photosynthesis and Plant Productivity Effect of UV-B and visible light on the reaction mechanism of photosynthetic water cleavage Renger,G.;H.J.Eckert
  35. Acta Protozool. v.34 Effects of UV-B irradiation on growth, survival, pigmentation and nitrogen metabolism enzymes in cyanobacteria Sinha,R.P.;H.D.Kumar;A.Kumar;D.P.Hadar
  36. J. Plant Physiol. v.153 Effects of ultraviolet-B radiation in three rice field cyanobacteria Sinha,R.P.;D.P.Hader https://doi.org/10.1016/S0176-1617(98)80232-0
  37. Science v.255 Ozone depletion: Ultraviolet radiation and phytoplankton biology in Antarctic waters Smith,R.C.;B.B.Preselin;K.S.Baker;R.R.Bidigare;N.P.Boucher;T.Coley;D.Karentz;S.MacIntyre;H.A.Matlick;D.Menzies;M.Ondrusek;Z.Wan;K.J.Waters https://doi.org/10.1126/science.1546292
  38. Biochem. Biophys. Acta v.1020 Effects of supplementary ultraviolet-B radiation on photosynthesis in Pisum sativum Strid,A.;W.S.Chow;J.M.Anderson https://doi.org/10.1016/0005-2728(90)90156-X
  39. Physiol. Plant v.52 Effects of supplemental UV-B radiation on growth and leaf photosynthetic reactions of soybeen(Glycine max) Vu;C.V.;L.H.Allen;L.A.Garrard https://doi.org/10.1111/j.1399-3054.1981.tb06054.x
  40. Bull. Environ. Contam. Toxicol. v.23 Cadmium toxicity to freshwater algae Wong;P.T.S.;G.Burnison;Y.K.Chou https://doi.org/10.1007/BF01769992