Restorative Dentistry and Endodontics

  • 제28권2호
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  • Pages.178-183
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  • 2003
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  • 2234-7658(pISSN)
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  • 2234-7666(eISSN)
대한치과보존학회 (The Korean Academy of Conservative Dentistry)
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중합효소연쇄반응법을 이용한 급성 치수 및 치근단 질환의 병원성 세균의 동정

IDENTIFICATION OF PUTATIVE PATHOGENS IN ACUTE ENDODONTIC INFECTIONS BY PCR BASED ON 16S rDNA

  • 김지훈 (조선대학교 치과대학 보존학교실) ;
  • 유소영 (조선대학교 치과대학 구강생화학교실, 구강생물학연구소) ;
  • 임선아 (조선대학교 치과대학 구강생화학교실) ;
  • 국중기 (조선대학교 치과대학 구강생화학교실, 구강생물학연구소) ;
  • 임상수 (조선대학교 치과대학 보존학교실) ;
  • 박슬희 (조선대학교 치과대학 보존학교실) ;
  • 황호길 (조선대학교 치과대학 보존학교실, 구강생물학연구소)
  • Kim, Ji-Hoon (Department of Conservative Dentistry, College of Dentistry, Chosun University) ;
  • Yoo, So-Young (Department of Oral Biochemistry, College of Dentistry, Chosun University) ;
  • Lim, Sun-A (Department of Oral Biochemistry, College of Dentistry, Chosun University) ;
  • Kook, Joong-Ki (Department of Oral Biochemistry, College of Dentistry, Chosun University) ;
  • Lim, Sang-Soo (Department of Conservative Dentistry, College of Dentistry, Chosun University) ;
  • Park, Seul-Hee (Department of Conservative Dentistry, College of Dentistry, Chosun University) ;
  • Hwang, Ho-Keel (Department of Conservative Dentistry, College of Dentistry, Chosun University)
  • 발행 : 2003.03.01
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초록

The purpose of this study was to investigate the frequency of 7 putative pathogens in endodontic infections. The specimens were collected from infected pulpal tissue of patients who were referred for root canal treatment to the department of conservative dentistry, Chosun University Samples were collected aseptically using a barbed broach and a paper point. The cut barbed broaches and paper points were transferred to an eppendorf tube containing 500 ml of 1 X PBS. DNAs were extracted from the samples by direct DNA extraction method using lysis buffer (0.5% EDTA, 1% Triton X-100). Identification of 7 putative pathogens was performed by PCR based on 16S rDNA. The target species were as follows : Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Bacteroides forsythus, Actinobacillus actinomycetemcomitans, and Treponema denticola. Our data revealed that the prevalence of P. endodontalis was found in 88.6% (39/54), P. ginivalis 52.3% (23/44), P. nigrescens 18.2% (8/44), P intermedia 15.9% (7/44) B. forsythus 18.2% (8/44), A. actinomycetemcomitans 3.3% (1/44), T. denticola 25% (l1/44) of the samples. The high prevalence of P. endodontalis and P. ginivalis suggests that they may play an important role in the etiology of endodontic infections.

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