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Comparison of the Antioxidative Abilities of Commercial Gal Geun Tang

시판 갈근탕의 항산화능의 비교

  • Kim, Dong-Ryul (Dept. of Food Science and Biotechnology, Kyungnam University) ;
  • Kwak, Gael-Sun (Dept. of Food Science and Biotechnology, Kyungnam University) ;
  • Jeong, Seok-Moon (Dept. of Food Science and Biotechnology, Kyungnam University) ;
  • Lee, Seung-Cheol (Dept. of Food Science and Biotechnology, Kyungnam University) ;
  • Ha, Jung-Uk (Dept. of Food Science and Biotechnology, Kyungnam University)
  • 김동렬 (경남대학교 생명과학부 식품생물공학) ;
  • 곽결순 (경남대학교 생명과학부 식품생물공학) ;
  • 정석문 (경남대학교 생명과학부 식품생물공학) ;
  • 이승철 (경남대학교 생명과학부 식품생물공학) ;
  • 하정욱 (경남대학교 생명과학부 식품생물공학)
  • Published : 2003.07.01

Abstract

Each 3 g of five different commercial Gal Geun Tang extractive granules was mixed with 97 $m\ell$ of methanol or water for 1.5 hour at room temperature and filtered through a Whatman No. 1 filter paper, and the filtrate was used to determine antioxidant activity. Antioxidative abilities of each extracts were compared by measuring total phenol content (TPC), electron donating ability (EDA), inhibition of lipid peroxidation, and reducing power Average TPC of methanol extracts from five samples was 0.14 mM within the range of 0.07~0.23 mM, while that of water extracts was 0.23 mM, higher than that of methanol extracts. Average EDA of the methanol extracts was 85.54%, higher than that of the 0.1% BHT (72.26%). Average EDA values of the water extracts were 69.7%. Average value of inhibition against lipid peroxidation of the methanol extracts was 26.95%, and the range of five samples was 17.02~37.36%. Inhibition against lipid peroxidation of the water extracts showed relatively low value, 18.62%. Reducing power of the methanol extracts was 0.77 unit, which was 73.1% of 0.1% BHT, and that of water extracts was 0.88 unit. These results indicate that commercial Gal Geun Tang shows high antioxidant ability, though there are some differences depending on extract solvent and manufacturing company.

5개 회사의 시판 갈근탕 엑스과립 3 g에 물과 메탄올 97$m\ell$를 각각 추출용매로 하여 추출물을 조제한 다음 항산화능을 비교 조사하였다. 탄닌산을 기준으로 하여 측정한 총페놀 함량은 메탄올 추출물의 경우 평균 0.14 mM이었고, 제조회사에 따라 0.07 mM에서 0.23 mM의 범위를 보였다. 물 추출물의 총페놀 함량은 5개 제품의 평균값이 0.23 mM로서 메탄올 추출물에 비해 상당히 높았고, 0.12 mM에서 0.28 mM의 분포를 나타내었다. DPPH에 대한 전자공여능은 메탄올 추출물의 평균값이 85.54%로 대조구인 0.1% BHA의 72.26%보다 높은 수준을 보였고, 물 추출물의 평균값은 69.7%로서 대조구인 0.1% 아스코르빈산의 92.7%에 비해 낮게 측정되었다. TBARS 측정 에 의한 지질 과산화 저해율은 메탄올 추출물의 평균 저해률은 26.95%이었고, 전체적으로 17.02~37.36%의 범위를 보였다. 한편 물 추출물의 지질 과산화 저해율은 평균 18.62%로 메탄올 추출물에 비해 낮게 측정되었다. 또한, 메탄올 추출물의 환원력 평균값은 0.77단위로 대조구의 환원력 1.05단위의 73.1% 수준이었고, 물 추출물은 평균 0.88 단위이었다. 따라서, 시판 갈근탕 엑스과립은 제조 회사와 추출용매에 따라 항산화능의 차이가 나타남을 알 수 있었다.

Keywords

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