Journal of Microbiology

  • 제41권2호
  • /
  • Pages.73-77
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  • 2003
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Characterization of the Proteolytic Activity of Bacteria Isolated from a Rotating Biological Contactor

  • In Jae park (Department of Microbiology, College of Sciences, Daejeon University) ;
  • Yoon, Jerng-Chang (Department of Microbiology, College of Sciences, Daejeon University) ;
  • Park, Seong-Joo (Department of Microbiology, College of Sciences, Daejeon University) ;
  • Kim, Eung-Ho (Major in Civil Engineering, School of Urban ＆ Civil Engineering, College of Engineering, Hong-Ik University) ;
  • Cho, Yeon-Jae (Seil Technology co., LTD) ;
  • Shin, Kwang-Soo (Department of Microbiology, College of Sciences, Daejeon University)
  • 발행 : 2003.06.01
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초록

Four proteolytic bacteria were isolated and identified from a rotating biological contactor based on Bacillus. The four isolates, Ni 26, 36, 39 and 49 were identified as B. vallismortis, B. subtilis, Aeromonas hydrophila and B. amyioliquefaciens, respectively, based on their biochemical properties and 16S rDNA sequence analyses. The optimal proteolytic activity was observed in the temperature and pH ranges of 40-70$^{\circ}C$ and 8.0-8.5, respectively. The proteolytic activities of all the isolates were partially inhibited by phenylmethylsulfonylfluoride (PMSF), and the isolates Ni 26, Ni 39 and Ni 49 were inhibited by the metalloprotease inhibitor, 1,10-phenanthroline. Zymographic analyses of the culture supernatants revealed the presence of at least two pretenses in all isolates.

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