생명과학회지 (Journal of Life Science)

  • 제13권3호
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  • Pages.355-358
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  • 2003
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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잔디 뿌리병 병원균인 Rhizoctonia solani의 성장을 저해하는 미생물 선발

Screening of Potent Biofungicide for the Growth Inhibition of Soilborne Pathogenic Fungi, Rhizoctonia solani

  • 이은열 (경성대학교 공과대학 식품공학과) ;
  • 이재화 (신라대학교 공과대학 생명공학과)
  • 발행 : 2003.06.01
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초록

식물 병원성 진균인 Rhizoctonia solani에 대한 길항능이 있는 Trichoderma 계열의 미생물 균주를 선별하였다. R. solani의 성장을 저해하는 능력이 우수한 균주를 선별하기 위하여 일차적으로 PDA 평판에서 inhibition zone을 측정하였고, 병원성 진균의 세포벽을 용해시킬 수 있는 세포외 효소인 glucanase 및 chitinase 활성을 분석하였다. 4∼5 mm 이상의 inhibition zone을 보여주었고, glucanase 및 chitinase 활성이 우수한 Trichoderma sp. UK-3와 T. viride 균주들을 선별할 수 있었다.

Various Trichoderma spp. were evaluated for the development of biofungicides to control soilborne pathogen, Rhiztonia solani, Various Trichoderma spp. were initially tested for their ability to inhibit growth of R. solani by inhibition zone test. Inhibition zones of 3∼5 mm toward R. solani were detected on PDA agar plates. The parasitic activity of strains, the activities of cell-wall-degrading enzymes such as glucanases and chitinases, were also evaluated. Highest activities of glucanase and chitinase were 3.5 U/ml and 0.9 U/ml, respectively, Isolated Trichoderma spp. also exhibited good growth with currently used agrochemicals, which represents that the isolated biofungicides can be mutually used with agrochemicals.

키워드

참고문헌

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