Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Induction of Conidioma Formation of the White Root Rot Fungus in Axenic Culture under Near Ultraviolet Light Radiation

NUV 광조사에 의한 배나무 흰날개무늬병의 분생자과 형성

  • 이중섭 (농촌진흥청 원예연구소 원예환경과) ;
  • 박종한 (농촌진흥청 원예연구소 원예환경과) ;
  • 한경숙 (농촌진흥청 원예연구소 원예환경과) ;
  • 최용문 (농촌진흥청 원예연구소 원예환경과) ;
  • 송본직행 (일본 농업환경기술연구소)
  • Published : 2003.03.01
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Abstract

Conidiomata of the Rosellinia necatrix were induced in axenic culture under near ultraviolet light radiation. Pieces of sterilized Japanese pear twigs were placed on the 7 days-old oatmeal agar culture in plates. The plates were further incubated far 5 days and then illuminated by under near ultraviolet light radiation. Syn-nemata were developed on twigs in 19 out of 20 isolates tested within 5 weeks, and conidia were observed in 12 out of the 19 isolates. The synnemata and conidia produced were morphologically identical to those of Dematophora necatrix.

흰날개무늬병원균의 분생자과를 형성시키기 위하여 NUV 광을 조사하면서 무균적으로 배양하였다. 흰날개무늬병원균을 Oatmeal Agar 배지상에 7일동안 배양시킨 후 살균처리된 배나무 가지를 을려놓아 2$0^{\circ}C$실온에서 5일 동안 NUV 광을 조사하면서 배양하였다. 그 결과, 배나무 가지표면에 분생자병속이 처리 5주후에 95% 형성시킬 수 있었다. 또한, 유기된 분생자병속으로부터 63%의 분생자가 형성되는 것을 관찰하였다. 새롭게 형성된 분생자병속과 분생자의 형태적 특성은 Dematophora necatrix에 대하여 보고된 것과 동일하였다.

Keywords

References

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