Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Melanin Biosynthesis Inhibitory Activities of Coumarins Isolated from Angelica polymorpha MAXIM

궁궁이(Angelica polymorpha MAXIM)로부터 분리한 Coumarin 계열 화합물의 Melanin 생합성 억제 활성

  • Published : 2003.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

During the screening for inhibitors of melanin biosynthesis from plant extract, Angelica polymorpha MAXIM which showed a high level of inhibition was selected. The inhibiting substances were purified form methanol extract of Angelica polymorpha MAXIM followed by silica gel column chromatography and HPLC. The inhibitors were identified as heraclenin, isosaxalin and heraclenol 3'-Me ether, by spectrescopic methods of ESI-MS, H-NMR, C-NMR, DEPT, HMQC and HMBC. These compounds did not have mushroom tyrosinase inhibitory activity, but showed a highly potent melanin biosynthesis inhibition zone in the plate culture of Streptomyces bikiniensis, a bacterium used as an indicator organism in this work. These compounds did not show any growth inhibition against S. bikiniensis at the same concentration of melanin biosynthesis test.

Melanin 생합성에 key enzyme인 tyrosinase 억제가 melanin색소 억제에 주된 요인으로 알려져 있지만, 생합성기작에는 여러 다른 요인들이 작용하기 때문에 일차적인 탐색 단계에서 tyrosinase 저해 활성뿐만 아니라 S. bikiniensis의 melanin생합성 억제 연구를 병행하여 천연물을 탐색하였다. 그 중 선택된 궁궁이 (Angelica poiymorpha MAXIM)에서 3가지 물질을 분리하였으며, 3가지 물질 모두가 100$\mu\textrm{g}$/$m\ell$ 이상 농도에서도 tyrosinase억제 활성이 나타나지 않는 반면, S. bikiniensis melanin 생합성 저해 효과를 보여 주었다. 동일 농도에서 S. bikinieffsis의 생장에는 영향을 미치지 않았다.

Keywords

References

  1. Ann. Rev. Phytophathol. v.24 Biosynthesis and function of fungal melanin Bell,A.A.;M.H.Weeler
  2. Phytochemistry v.12 Coumarins nad other components of Afraegle paniculata Ezekiel,K.A.
  3. Biochem. J. v.157 Mammalian tyrosinase Hearing,V.J.;T.M.Ekel
  4. Fitoterapia v.74 A new dicoumarinyl ether and two rare furocoumarins from Ruta montana Kabouche,Z.;N.Benkiki;E.Seguin;C.Buuneau
  5. J. Invest. Dermatol. v.100 Pigment production in murine melanoma cells is regulated by tyrosinase, tyrosinase-related protein 1(TRP 1), dopachrome tautomerase (TRP 2) and a melanogenic inhibitor Kameyama,K.;T.Takemura;Y.Hamada;C.Sakai;S.Kon-doh;S.Nishi-yama
  6. 신물질탐색 Kim,C.J.;H.K.Lee;Y.H.Kim;K.S.Kim;Y.B.Seo;H.S.Lee;B.S.Yun
  7. Kor. J. Appl. Microbiol. Biotechnol. v.21 Tyrosinase-inhibiting isoflavonoids produced by Streptomyces sp. 20747 Lee,C.H.;H.K.Chun;Y.B.Seu;Y.H.Kho
  8. Kor. J. Appl. Microbiol. Biotechnol. v.23 Production of isocyanide inhibitor of melanin biosynthesis by Trichoderma sp. MR-93 Lee,C.H.;H.K.Chun;M.C.Chung;H.J.Lee;K.S.Bae;Y.H.Kho
  9. Kor. J. Appl. Microbiol. Biotechnol v.23 MR304-1, A melanin synthesis inhibitor produced by Trichoderma harzianum Lee.C.H.;M.C.Chung;H.J.Lee;K.H.lee;Y.H.Kho
  10. J. Antibiotics v.50 MR567 and MR566B, new melanin synthesis inhibitors produced by Trichoderma harzianum I. taxonomy, fermentation, isolation and biological activities Lee,C.H.;M.C.Chung;H.J.Lee;K.S.Bae;Y.H.Kho
  11. J. Antibiotics v.50 MR567 and MR566B, new melanin synthesis inhibitors produced by Trichoderma harzianum II. physico-chemical properties and structural elucidation Lee,C.H.;K.Hirotuki;M.C.Chung;H.J.Lee;J.K.Hong;J.S.Yoo;Y.H.Koh
  12. Kor. J. Pharmacog. v.13 Coumarin constituents from the roots of Anelica koreana Max Lee,C.K.;W.S.Woo
  13. Yakhak Hoeji v.41 The screening of the inhibitory compounds on tyrosinase activity from the natural product Lee,S.H.;J.S.Park;S.Y.Kim;S.R.Chung
  14. 동서의학연구소 논문집 v.2000 Effects of Licorice extract on proliferation and melanization in cultured normal human melanocytes Lim,T.W.;J.W.Lee;M.H.Lee
  15. Fitoterapia v.72 Coumarins from the aerial part of Halocnemum strobilaceum Miftakhoca,A.F.;G.S.Burasheca;Z.A.Abilov;V.U.Ahmad;M.Zahid
  16. Pigment Cell Res. v.1 Induction of melanogenesis suppression : Cellular pharmacology and mode or differential action Mishima,Y.;S.Hatta;M.Inazu
  17. Phytochemistry v.13 Coumarin constituents of Selinum tenuifolium Narayan,A.;G.Dipali;C.Ashim
  18. J. Biol. Chem v.268 Melanin biosynthesis patterns following hormonal stimulation Pilar,A.;U.Kazunori;K.Takeshi;T.Katsuhiko;Hearing,V.J.
  19. Melanin and melanogensis Prota,G.
  20. Pigment Cell Res. v.13 Cyclic AMP a key messenger in the regulation of skin pigmentation Roser,B.;B.Robert
  21. Ann. N. Y. Acad. Sci. v.885 α-MSH can control the essential cofactor 6-terahydrobiopterin in melanogensis Schallreuter,K.U.;J.Moore;D.J.Tobin;N.J.Gibbons;H.S.Marshall;T.Jenner;W.D.Beazley;J.M.Wood
  22. Phytochem. Anal. v.14 HPLC-NMR/HPLC-MS analysis of the bark extract of Stauranthus perforatus Setzer,W.N.;B.Vogler;R.B.Bates;J.M.Schmidt;C.W.Dicus;P.Nakkiew;W.A.Harber
  23. J. Antibiotics v.44 A new screening method for melanin biosynthesis inhibitors using Streptomyces bikiniensis Tomit,K.N.;N.Oda;M.Kamei;T.Miyaki;T.Oki
  24. Biotechnol. Tech. v.9 Screening of melanin biosynthesis inhibitors from marine microalgae using Streptomyces bikiniensis bioassay Wachi,Y.;K.Sode,;K.Horikoshi;H.Takeyama;T.Matsunaga
  25. Phytochem. Phytobiol. v.65 Induced melanin reduces mutations and cell killing in mouse melanoma Weixiong,L.;Z.H.Helene