Biotechnology and Bioprocess Engineering:BBE

  • 제8권2호
  • /
  • Pages.117-125
  • /
  • 2003
  • /
  • 1226-8372(pISSN)
  • /
  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Residence Time Distribution in the Chromatographic Column: Applications in the Separation Engineering of DNA

  • 발행 : 2003.04.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Experimental and theoretical works were performed for the separation of large polyelectrolyte, such as DNA, in a column packed with gel particles under the influence of an electric field. Since DNA quickly orient in the field direction through the pores, this paper presents how intraparticle convection affects the residence time distribution of DNAs in the column. The concept is further illustrated with examples from solid -liquid systems, for example, from chromatography Showing how the column efficiency is improved by the use of a n electric field. Dimensionless transient mass balance equations were derived, taking into consideration both diffusion and electrophoretic convection. The separation criteria are theoretically studied using two different Peclet numbers in the fluid and solid phases. These criteria were experimentally verified using two different DNAs via electrophoretic mobility measurements. which showed how the separation position of the DNAs varies in the column in relation to the Peg/Pef values of an individual DNA. The residence time distribution was solved by an operator theory and the characteristic method to yield the column response.

키워드

참고문헌

  1. Chem. Eng. Sci. v.35 Simultaneous intraparticle forced convection, diffusion and reaction in a porous catalyst I Nir,A.;L.Pisman
  2. Chem. Eng. Sci. v.44 The effect of intraparticle convection on nutrient transport in porous biologival pellets Stephanopoulos,G.;K.Tsiveriotis https://doi.org/10.1016/0009-2509(89)85139-5
  3. Bioechnol. Prog. v.9 Effect of inraparticle convection on the chromatography of biomolecules Frey,D.D.;E.Schweinheim;C.Horvath https://doi.org/10.1021/bp00021a006
  4. Chem. Eng. Sci. v.50 Linear driving force approximation for intraparticle diffusion and convection in permeable supports Carta,G. https://doi.org/10.1016/0009-2509(94)00206-7
  5. Ind. Eng. Chem. Res. v.32 Effect of intraparticle forced convection on gas desorption from fixed beds containing Large-pore adsorbents Lu,Z.;J.M.Lourero;M.D.Douglas;A.E.Rodrigues
  6. Biochemical Eng. J. v.1 Mass transfer mechanisms in Hyper D media for chromatographic protein separation de la Vaga, R.;C.Chenou;J.M.Loureiro;A.E.Rodrigues https://doi.org/10.1016/S1369-703X(97)00003-X
  7. Biochem. Eng. J. v.2 Dynamic behabior of a fixed-bed biofilm reactor: Analysis of the role of the intraparticle convective flow under biofilm growth Leitao,A.;A.Rodrigues https://doi.org/10.1016/S1369-703X(98)00011-4
  8. AIChE J. v.39 Solute retention in electrochromatography by electrically induced sorption Rudge,S.R.;S.K.Basak;M.R.Ladish https://doi.org/10.1002/aic.690390508
  9. Sep. Sci. Tech. v.33 A new approach to counteracting chromatographic electrophoresis Yang,T.;C.J.Lee;I.M.Chu https://doi.org/10.1080/01496399808545907
  10. Electrochemical System Newman,J.S.
  11. Sep. Sci. Tech. v.27 Electric field driven separations: Phenomena and applications Ptassinski,K.J.;P.J.A.M.Kerkhof https://doi.org/10.1080/01496399208019021
  12. Linear Operator Methods in Chemical Engineering with Applications to Transport and Chemical Reaction Systems Ramkrishna,D.;N.Amundson
  13. Chem. Eng. Sci. v.48 Application of self-adjoint operators to electrophoretic transport in a composite media Ⅱ Locke,B.;P.Arce;Y.Park https://doi.org/10.1016/0009-2509(93)80379-5
  14. First-order Partial Differential Equations: Theory and Application of Single Equation Rhee,H.;R.Aris;N.Amundson
  15. Biopolymer v.2 Electrophoresis of nucleic acids Olivera,B.;P.Baine;N.Davidson https://doi.org/10.1002/bip.1964.360020306
  16. Chem. Eng. Sci. v.46 Residence time distribution of inert and linearly adsorbed species in a fixed bed containing large-pore supports: Applications in separation engineering Rodrigues,A.E.;L.Zupping;J.M.Loureiro https://doi.org/10.1016/0009-2509(91)85145-N
  17. Science v.251 DNA: A model compound for solution studies of macromolecules Pecora,R. https://doi.org/10.1126/science.2000490
  18. AIChE J. v.9 Hindered transport of large molecules in liquid-filled pores Deen,W.M.
  19. Elements of Chemical Reaction Engineering Fogler,H.S.
  20. Biopolymer v.25 On the reptation theory of gel electrophoresis Slater,G.W.;J.Noolandi https://doi.org/10.1002/bip.360250305
  21. Bipolymer v.26 Electrophoretic mobility of λ phage DNA fragments in agarose gel Hervet,H.;C.P.Bean https://doi.org/10.1002/bip.360260512
  22. Biopolymer v.28 The biased reptation model of DNA gel electrophoresis: Mobility vs. molecular size and gel concentration Slater,G.W.;J.Noolandi https://doi.org/10.1002/bip.360281011