KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Characterization of Cell Cultures and Ginsenoside Production by Cultured Ginseng and Wild Mountain Ginseng

산삼과 재배인삼의 세포배양 및 Ginsenoside 생성 특성

  • 유병삼 (아주대학교 화학생물공학부) ;
  • 장문식 (아주대학교 화학생물공학부) ;
  • 변상요 (아주대학교 화학생물공학부)
  • Published : 2003.04.01
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Abstract

Established cell-line cultures of cultured and wild mountain ginseng were characterized and their abilities to produce ginsenoside were determined. Cell lines were made of calli induced from the roots of wild mountain ginseng and cultured ginseng(Panax ginseng). Suspension cultures of wild mountain ginseng and cultured ginseng showed different growth and ginsenoside production rate. Their specific growth rates were 0.067 and 0.0035 day-1 in spite of having the same sugar consumption rates, where cells from wild mountain ginseng grew almost twice as fast as those of cultured ginseng. Their respective abilities to produce ginsenoside, however, were 0.53 and 2.53 mg/L.day, which means cells from cultured ginseng produced around 5 times more than wild mountain ginseng.

산삼과 인삼의 세포주를 확립하고, 배양을 통하여 세포성장 및 탄소원 소모 경향과 이들이 생산하는 생리활성물질의(ginsenoside) 생성 특성을 비교 분석하였다. 세포주는 직접 산삼(KM2, KM3, KM5의 뿌리) 및 재배인삼(포천, 홍천, 영주, 무주와 금산에서 재배된 시료)으로부터 callus를 유도하고 장기간 배양하여 확립하였다. 산삼과 재배인삼 세포배양 결과 나타난 특성을 비교해 보면, specific growth rate($\mu$, day-1)는 각각 0.067과 0.035로서 산삼유래의 KM5 세포가 약 2배 정도 빨랐고, 반면에 ginsenoside 생성속도는 각각 0.53과 2.53 mg/L day로 재배인삼 세포에서 약 5배 더 높게 나타났다. 그런데 sugar consumption rate는 각각 1.51과 1.54 g/L any로 비슷한 수준으로 나타났는데, 이러한 결과들을 고찰해 볼 때, 기초 세포배양 조건에서는 KM5 보다 재배인삼인 KE세포에서 ginsenoside 생합성 대사가 더욱 활발하게 이루어지고 있음을 알 수 있었다.

Keywords

References

  1. Korean Ginseng Park, M. K.
  2. Ph.D. Dissertation, Dept. of Biotechnology, Ajou University Identification of Wild Mountain Ginsengs by Analysis of DNA, Proteome, and Bioactive Compounds and Characterization of its Cell Cultures Yoo, B. S.
  3. Mediz. Leningrad Panax ginseng Brekhman, I. I.
  4. Chem. Pharm. Bull. v.14 no.6 Protopanaxadiol a genuine sapogenin of Ginseng Saponins Shibata, S.;O. Tanaka;M. Sado;S. Tsushima;T. Oshawa https://doi.org/10.1248/cpb.14.595
  5. Bio/Technology v.1 Harvesting profitable products from plant tissue culture Curtin, M. E. https://doi.org/10.1038/nbt1083-649
  6. Process Biochem. v.33 no.7 Enhancement of taxol production by in situ recovery of product Kwon, I. C.;Y. J. Yoo;J. H. Lee;J. O. Hyun https://doi.org/10.1016/S0032-9592(98)00037-5
  7. Experientia v.42 Formation of plants from callus cultures of Ginseng(Panax ginseng) Furuya, T.;T. Yoshikawa;K. Ushiyama;H. Oda https://doi.org/10.1007/BF01952464
  8. Nature v.284 In vitro flowering of bmbryoids derived from mature root callus of Ginseng(Panax ginseng) Chang, W. C.;Y. I. Hsing https://doi.org/10.1038/284341a0
  9. Korean J. Plant Tissue Culture v.23 no.4 Antioxidative activity in callus cultures of Ginseng(Panax ginseng C. A. Meyer) Yang, D. C.;J. C. Park;W. S. Kwon;K. H. Choi;K. T. Choi
  10. Korean J. Ginseng Sci. v.12 no.1 Studies on the embryo culture of Korean Ginseng 1. Effects of growth regulators on adventitious bud formation and flower Emergence Chung, C. M.;Y. T. Kim;J. S. Jo
  11. Korean J. Ginseng Sci. v.5 no.1 Root and shoot formation in explant and callus derived from root and Cotyledon of Cinseng (Panax ginseng C. A. Meyer) Choi, K. T.;M. W. Kim;H. S. Shin
  12. Cell Culture and Somatic Cell Genetics of Plants v.5 Furuya, T.;Indra K. Vasil(ed.)
  13. Chem. Pharm. Bull. v.43 no.1 Simultaneous analysis of Saponins in Ginseng Radix by high performance liquid chromatography Samukawa, K.;H. Yamashita;H. Matsuda;M. Kubo https://doi.org/10.1248/cpb.43.137
  14. Palnt Cell Reports v.6 no.1 Saponin production by cultures of Panax ginseng transformed with agrobacterium rhizogenes Yoshikawa, T.;T. Furuya
  15. Korean J. Biotechnol. Bioeng v.4 no.3 Induction and culture of hairy root from Ginseng (Panax ginseng C. A. Meyer) roots discs by Agrobacterium rhizognes Hwang, B.;K. M. Ko
  16. Korean J. Biotechnol. Bioeng. v.5 no.3 Ginsenoside production by hairy root culture of Paanax ginseng transformed with Agrobacterium rhizogenes Ko, K. S.;I. O. Heo;J. S. Koh;W. J. Lee
  17. Korena J. Plant Tissue Culture v.20 no.1 Production of secondary metabolites from hairy root of Panax ginseng transformed by Agrobacterium rhizogenes Ko, K. M.;J. C. Ahn;S. J. Hwang;Y. H. Kang;B. Hwang
  18. Plant Cell Reports v.12 Growth pattern and Ginsenoside production of Agrobacterium- transformed Panax ginseng roots Inomata, S.;M. Yokoyama;Y. Gozu;T. Shimizu;M. Yanagi
  19. Korean J. Biotechnol. Bioeng. v.9 no.2 High yield Saponin production by mass cultures of Ginseng transformed tissue I. Induction, culture of transformed tissue and selection of high-Saponin-producing clones in Ginseng Lee, J. S.;K. M. Ko;J. C. Ahn;D. G. Bai;K. Y. Park;S. R. Ko;B. Hwang
  20. Enzyme and Microbial Technology v.21 Hyperproduction of Ginseng Saponin and polysaccharide by high density cultivation of panax notoginseng cells Zhang, Y. H.;J. J. Zhong https://doi.org/10.1016/S0141-0229(96)00224-4
  21. Applied Biochemistry and Biotechnology v.75 Production of Ginseng Saponin and polysaccharide by cell cultures of panax notoginseng and Panax ginseg effect of plant growth regulators Zhong, J. J. https://doi.org/10.1007/BF02787779
  22. Process Biochem. v.33 no.1 Phosphate effect on production of Ginseng Saponin and polysaccharide by cell suspention cultures of Panax ginseng and Panax quinquefolium Liu, S.;J. J. Zhong https://doi.org/10.1016/S0032-9592(97)00064-2
  23. Process Biochem. v.33 no.6 Effects of nitrogen source on the production of Ginseng Saponin and polysaccharide by cell culture of Panax quinquefolium Zhong, J. J.;S. J. Wang https://doi.org/10.1016/S0032-9592(98)00030-2