DOI QR코드

DOI QR Code

한국산 무 추출물의 곰팡이 병균에 대한 항진균성

Antifungal Activity of Korean Radish (Raphanus sativaus L) Extracts Against Pathogenic Plant

  • Won, Hwang-Cher- (Department of Environmental microbiology, Handong University)
  • 발행 : 2003.04.01

초록

국내산 무의 추출물을 여러가지 Column과 HPLC를 이용하여 잿빛곰팡이 병원균에 대한 항 진균성 물질을 분리하였다. 투석튜브를 이용한 분리에서 이물질이 3.5kDa 이하의 저분자물질이며 또한 내열성 물질임을 확인하였다. 이물질들은 최소한 5종의 물질로 구성되어 있으며 이중 가장 항진균성이 강한 물질을 HPLC를 이용하여 순수 분리하였으며 잿빛곰팡이병에 감염된 식물에 적용한 결과 농약과 비슷한 효과를 나타내어 환경 친화적인 항진균 물질로서 사용가능성을 확인하였다.

A study of the anti-fungal properties in Korean radish was conducted using a variety of purification procedures such as Extrelut column, RP(Reverse Phase) Cl8 Column Chromatography, HPLC etc. to separate anti-fungal substances from Korean radish juices to test them against a common gray mold called Botrytis cenerea. Dialysis tube operation showed that these substances were presumably thermostable compounds with low molecular mass (less than 3.5 kDa). Differences of anti-fungal activities depending upon types of radishes used did not show any noticeable variation. The antifungals were presumably composed of more than 5 compounds. Among these, the most anti-fungal fraction was analyzed by HPLC in which one peak was obtained. Disease-affected plants were inoculated with 10mg of Extrelut fraction and results showed similar anti-fungal activity to pesticides suggesting possible usage of these substances as environmentally friendly antibiotics.

키워드

참고문헌

  1. J. Microbiol. Biotechnol. v.10 Isolation of bacterial strain antagonistic to Pyricularia oryzae and its made of antifungal action Bae,D.W.;J.T.Lee;D.Y.Son;E.S.Lee;H.K.Kim
  2. J. Microbiol. Biotechnol. v.10 Purification and characterization of a novel antifungal protein from Paenibacillus macerans PMI antagonistic to rice blast fungus Pyricularia oryzae Bea,D.W.;Y.S.Kawk;J.T.Lee;D.Y.Son;S.S.Chun;H.K.Kim
  3. EMBO J. v.7 Leaf-specific thionins of barleyanovel class of cell wall proteins toxic to plantpathogenic fungi and possibly involved in the defence mechanism of plants Bohlmann,H.;S.Clausen,;S.Behnke;H.Giese;H.Hiller;U.Reimann-Philipp;G.Schrader;V.Barkholt;K.Apel
  4. Biochemistry v.31 Antimicrobial peptides from Amaranthus cauatus seeds with sequence homology to the cysteine/glycine-rich domain of chtin-binding proteins Broekaert,W.F.;W.Marien;F.R.Terras;M.F.De Bolle;P.Proost;J.Van Damme;L.Dillen;M.Claeys;S.B.Rees;J.Vanderleyden;B.P.Cammue https://doi.org/10.1021/bi00132a023
  5. J. Biol. Chem. v.267 Isolation and chracterization of a novel class of plant antimicrobial peptides from Mirabilis jalapa L. seeds Cammue,B.P.;M.F.De Bolle;F.R.Terras;P.Proost;J.Van Damme;S.B.Rees;J.Vanderleyden;W.F.Broekaert
  6. J. Microbiol. Biotechnol. v.9 Purification and structure determination of antifungal phospholipids from a marin Streptomyces Cho,K.W.;Y.W.Seo;T.M.Yoon;J.H.Shin
  7. J. Mol. Biol. v.279 Determination of the three-dimensional solution structure of Raphanus sativus antifungal protein 1 by 1H NMR Fant,F.;W.Vranken;W.Broekaert;F.Borremans https://doi.org/10.1006/jmbi.1998.1767
  8. Appl. Microbiol. v.23 Susceptibility of phytopathogenic bacteria to wheat purothionins in vitro Fernandez de Caleya R.;B.Gonzalez-Pascual;F.Garcia-Olmedo;P.Carbonero
  9. J. Microbio. Biotechnol. v.8 Molecular cloning and sequence of URA5 gene encoding orotate phosphoribosyl(ORPTase)from entomopathogenic fungus, Metarhizium anisopliae Hwang,C.W.;D.K.Lee;S.C.Kang
  10. E. coil. Agri. Chemi. Biotech. v.4 Cloning of chitinase gene of Xanthomonas sp. isolated from soil and its expression Hwang,C.W.;H.S.Kim;K.Y.Seong;M.Y.Eun
  11. J. Microbio. Biotechnol. v.7 A partial nucleotide sequence of chitin synthase(CHS) gene from rice blast fungus, Pyricularia oryzae and its cloning Hwang,C.W.;I.C.Park;W.H.Yeh;M.Takagi;J.C.Ryu
  12. J. Microbiol. Biotechnol. v.10 Antimicrobial effectiveness of pine needle extract on food born illness bacteria Kim,K.Y.;M.Davidson;H.J.Chung
  13. Entomophage v.40 Current development in microbial control of insect past and prospects for the early 21st centry Lacey,L.A.;M.Goettel https://doi.org/10.1007/BF02372677
  14. J. Biol. Chem. v.266 Biochemical and molecular characterization of three barley seed proteins with antifungal properties Leah,R.;H.Tommerup;I.Svendsen;J.Mundy
  15. Phytochemistry v.12 Some properties of a β1,3-Glucanase from RYE Manners,D.J.;J.J.Marshall https://doi.org/10.1016/S0031-9422(00)84441-6
  16. FEBS Lett. v.368 Isolation and characterization of plant defensin from seeds of Asteraceae, Fabaceae, Hippocastanaceae and Saxifragaceae Osborn,R.W.;G.W.De Samblanx;K.Thevisson;I.Goderis;S.Torrekens;F.Van Leuven;S.Attenborough;S.B.Rees;W.F.Broekaert https://doi.org/10.1016/0014-5793(95)00666-W
  17. Anticancer Drug v.10 An antimelanoma-barley ribosome inactivating protein conjugate is cytotoxic to melanoma cells in vitro Ovalia,M.;C.C.Hager;T.N.Oeltmann https://doi.org/10.1097/00001813-199908000-00007
  18. J. Microbio. Biotechnol. v.11 New antimicrobial activity from Korean radish seeds(Raphanus sativus L.) Park,J.H.;H.K.Shin;C.W.Hwang
  19. J. Invertebr. Pathol. v.67 Future perspectives on insect pest management: Engineering the pest Pfeifer,T.A.;T.A.Grigliatti https://doi.org/10.1006/jipa.1996.0017
  20. Biochim. Biophys. Acta. v.880 Isolation and partial charaterization of two antifungal proteins from barley Roberts,W.K.;C.P.Selitrennikoff https://doi.org/10.1016/0304-4165(86)90076-0
  21. J. Microbiol. Biotechnol. v.10 New polyene macrolide antibiotics from Streptomyces sp. M90025 Seo,Y.W.;K.W.Cho;H.S.Lee;T.M.Yoon;J.H.Shin
  22. J. Biol. Chem. v.267 Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds Terras,F.R.G.;H.M.E.Schoofs;M.F.C.De Bolle;F.Van Leuven;S.B.Rees;J.Vanderleyden;B.P.A.Cammue;W.F.Broekaert
  23. FEBS Lett. v.316 A new family of basic cysteinerich plant antifungal proteins from Brassicaceae species Terras,F.R.G.;S.Torrekens;F.Ven Leuven;R.W.Osborn;J.Vanderleyden;B.P.A.Cammue;W.F.Broekaert https://doi.org/10.1016/0014-5793(93)81299-F
  24. Mol. Plant-Microbe Interact v.4 A new family of plant antifungal proteins Vigers,A.J.;W.K.Roberts;C.P.Selitrennikoff https://doi.org/10.1094/MPMI-4-315
  25. Biochem. Biophysi. Res. Commun. v.269 Dolichin, a new chitinase-like antifungal protein isolated from flied beans (Dolichos lablab) Ye,X.Y.;H.X.Wang;T.B.Ng https://doi.org/10.1006/bbrc.2000.2115

피인용 문헌

  1. Screening of Antifungal Activities of Medicinal Plants for the Control of Turfgrass Fungal Disease vol.2, pp.1, 2013, https://doi.org/10.5660/WTS.2013.2.1.070