생명과학회지 (Journal of Life Science)

  • 제13권2호
  • /
  • Pages.197-205
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  • 2003
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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부산시 상수원수와 수돗물에서의 수인성 장관계 바이러스 분포조사

Distribution of Waterborne Enteric Viruses in Raw Water and Tap Water in Busan Metropolitan City

  • 박홍기 (부경대학교 생물공학과) ;
  • 정은영 (부산광역시 상수도사업본부 수) ;
  • 이유정 (부산광역시 상수도사업본부 수) ;
  • 정종문 (부산광역시 상수도사업본부 수) ;
  • 최동훈 (부산광역시 상수도사업본부 수) ;
  • 손희종 (부산광역시 상수도사업본부 수) ;
  • 권기원 (부산광역시 상수도사업본부 수) ;
  • 홍용기 (부경대학교 생물공학과)
  • 발행 : 2003.04.01
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초록

미국 EPA (Environment Protection Agency)와 환경부에서 규정하고 있는 표준검출법인 총세포배양법 (Total Culturable Virus Assay : TCVA)을 이용하여 부산시 상수원수 및 정수장을 대상으로 2001년 7월에서 2002년 11월까지 바이러스 검사를 실시하였다. 검사결과 21개의 상수원수 시료 중 13개 시료에서 CPE (cytopathic effect)가 확인되어 61.9%의 양성률을 보였으며, 정수 및 수도꼭지수에서는 모두 검출되지 않았다. 또한, 검출된 바이러스를 계절별로 보면 주로 여름철과 초겨울에 분포하는 특성을 보였다. 검출된 바이러스는 TCVA-MPN 방법에 의해 1.92-9.70 MPN/100 L의 범위로 전량 되었으며, 면역형광법 (Immunofluorescent assay)에 의해 human poliovirus type 1과 enterovirus종으로 동정되었다.

We detested waterborne enteric viruses from the raw water and tap water in Busan metropolitan city by the total culturable virus assay of EPA standard method. According to the results of survey from July 2001 to November 2002, thirteen out of twenty one in raw water samples were positive (61.9%) for enteric viruses and all of the treated water and tap water samples were negative. The enteric viruses in raw water were mainly distributed through the summer to the earl y winter, suggesting the seasonal characteristics of virus distribution in water The titer of enteric viruses per 100 liters of the raw water was ranged from 1.92 to 9.70 MPN by TCVA-MPN program. The isolated viruses were identified as either human poliovirus type 1 or enteroviruses by the immunofluorescent assay.

키워드

참고문헌

  1. Appl. Environ. Microbiol. v.65 A strategy for detection of viruses in ground-water by PCR Abbaszadegan,M.;P.Stewart;M.Lechevallier
  2. Appl. Environ. Microbiol. v.59 Detection of enteroviruses in groundwater with the polymerase chain reaction Abbaszadegan,M.;M.S.Huber;C.P.Gerba;I.L.Pepper
  3. Standard Methods for the Examination of Water and Wastewater APHA
  4. Appl. Environ. Microbiol. v.66 Detection of astroviruses, enteroviruses and adenovirus type 40 and 41 in surface waters collected and evlauated by the information collection rule and integrated cell culture-nested PCR procedure C.D.Chapron;N.A.Ballester;J.H.Fontaine;N.F.Christine;A.B.Margolin https://doi.org/10.1128/AEM.66.6.2520-2525.2000
  5. Can. J. Microbiol. v.46 Detection of adenoviruses and enteroviruses in tap water and river water by reverse transcription multiplex PCR Cho,H.B.;S.H.Lee;J.C.Cho;S.J.Kim https://doi.org/10.1139/cjm-46-5-417
  6. The Korean J. of Microbiology v.38 Detection of Poliovirus in Water by Cell Culture and PCR Methods Cho,Y.H.;C.H.Lee
  7. ICR Microbial Laboratory Manual EPA
  8. Information Collection Requirements Rule Protozoa and Enteric Virus Sample Collection Procedure EPA
  9. Korean J. of Life Science v.10 An Effective Method for the Concentration and Detection of Enteroviruses from Water Samples by Combined Cell Culture-Polymerase Chain Reaction Jung,E.Y.;K.L.Jang
  10. Korean J. of Life Science v.10 Detection of Enteroviruses from Surface Water by Combined Cell Culture-PCR Jung,E.Y.;J.M.Jung;J.I.Ryoo;P.S.Shin;H.K.Jun;K.L.Jang
  11. Forum collection of waterborne viral analysis National survey research and significance from ICR method Jung,Y.S.
  12. Can. J. Microbiol. v.47 ICC/PCR detection of infectious enteroviruses and hepatitis A virus in environmental samples K.A.Reynolds;C.P.Gerba;I.L.Pepper https://doi.org/10.1139/cjm-47-2-153
  13. Appl. Environ. Microbiol. v.62 Detection of enteroviruses by an integrated cell culture PCR procedure K.A.Reynolds;C.P.Gerba;I.L.Pepper
  14. Wat. Res. v.36 Detection of infections enteroviruses and adenoviruses in tap water in urban areas in Korea Lee,S.H.;S.J.Kim https://doi.org/10.1016/S0043-1354(01)00199-3
  15. Standard for Drinking Water Treatment Ministry of Environment
  16. Standard Method for the Drinking Water Quality Ministry of Environment
  17. Water Quality Management for Health and Treatment related microorganisms National Institute of Environmental Research
  18. Wat. Res. v.26 N.Tani;K.Shimamoto;K.Ichmura;Y.Nishii;S.Tomita;Y.Oda https://doi.org/10.1016/0043-1354(92)90109-H
  19. Eutrophication of Waters; Monitoring, Assessment and Control OECD
  20. J. of the Korean Environmental Sciences Society v.8 The Relationship between Phytoplankton Productivity and Water Quality in Downstream of Nakdong River Park,H.K.;C.M.Chung;J.R.Bahk;Y.K.Hong
  21. Virology v.16 Cationic stabilization a new property of enterovirus Wallis,C.;J.L.Melinick
  22. Distribution of Viruses in Tap Water Water Quality Institute of Busan Metropolitan City
  23. A Study on the control and distribution of viruses in Tap Water Waterworks Research Institute of Seoul Metropolitan Government
  24. Research on expansion plan of water quality test items Waterworks Research Institute of Seoul Metropolitan Government
  25. Guidelines for Drinking-Water Quality WHO
  26. J. Med. Virol. v.6 Enzyme-linked immunosobant assay for detection and identification of coxsachie B antigen in tissue cultures and clinical specimens Yolken,R.H.;V.M.Torsch https://doi.org/10.1002/jmv.1890060107

피인용 문헌

  1. Detection and Distribution of Bacterial Pathogens in Raw Water and During Water Treatment Process by Polymerase Chain Reaction vol.17, pp.10, 2007, https://doi.org/10.5352/JLS.2007.17.10.1374
  2. A 5-year survey (2007-2011) of enteric viruses in Korean aquatic environments and the use of coliforms as viral indicators vol.57, pp.1, 2013, https://doi.org/10.1111/j.1348-0421.2012.00515.x