Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Distribution of Waterborne Enteric Viruses in Raw Water and Tap Water in Busan Metropolitan City

부산시 상수원수와 수돗물에서의 수인성 장관계 바이러스 분포조사

  • 박홍기 (부경대학교 생물공학과) ;
  • 정은영 (부산광역시 상수도사업본부 수) ;
  • 이유정 (부산광역시 상수도사업본부 수) ;
  • 정종문 (부산광역시 상수도사업본부 수) ;
  • 최동훈 (부산광역시 상수도사업본부 수) ;
  • 손희종 (부산광역시 상수도사업본부 수) ;
  • 권기원 (부산광역시 상수도사업본부 수) ;
  • 홍용기 (부경대학교 생물공학과)
  • Published : 2003.04.01
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Abstract

We detested waterborne enteric viruses from the raw water and tap water in Busan metropolitan city by the total culturable virus assay of EPA standard method. According to the results of survey from July 2001 to November 2002, thirteen out of twenty one in raw water samples were positive (61.9%) for enteric viruses and all of the treated water and tap water samples were negative. The enteric viruses in raw water were mainly distributed through the summer to the earl y winter, suggesting the seasonal characteristics of virus distribution in water The titer of enteric viruses per 100 liters of the raw water was ranged from 1.92 to 9.70 MPN by TCVA-MPN program. The isolated viruses were identified as either human poliovirus type 1 or enteroviruses by the immunofluorescent assay.

미국 EPA (Environment Protection Agency)와 환경부에서 규정하고 있는 표준검출법인 총세포배양법 (Total Culturable Virus Assay : TCVA)을 이용하여 부산시 상수원수 및 정수장을 대상으로 2001년 7월에서 2002년 11월까지 바이러스 검사를 실시하였다. 검사결과 21개의 상수원수 시료 중 13개 시료에서 CPE (cytopathic effect)가 확인되어 61.9%의 양성률을 보였으며, 정수 및 수도꼭지수에서는 모두 검출되지 않았다. 또한, 검출된 바이러스를 계절별로 보면 주로 여름철과 초겨울에 분포하는 특성을 보였다. 검출된 바이러스는 TCVA-MPN 방법에 의해 1.92-9.70 MPN/100 L의 범위로 전량 되었으며, 면역형광법 (Immunofluorescent assay)에 의해 human poliovirus type 1과 enterovirus종으로 동정되었다.

Keywords

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