생명과학회지 (Journal of Life Science)

  • 제13권2호
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  • Pages.168-174
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  • 2003
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Medilite 침출수에 의한 녹차 추출물의 항산화 활성연구

Studies on Antioxidative Activity of Green Tea Extracts in Medilite-Extraction Water

  • 차재영 (동아대학교 응용생명공학부) ;
  • 조영수 (동아대학교 응용생명공학부)
  • 발행 : 2003.04.01
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초록

천연 미네랄 성분을 함유한 medilite 침출수를 이용한 녹차 추출물에 의한 화학적 성분조성과 항산화 활성을 증류수 침출 녹차 추출물과 비교하였다. 항산화 활성은 DPPH법 및 microsone 생체막 지질 과산화물 생성정도를 나타내는 TBARS법으로 측정하였다. 녹차 추출물의 총 폴리페놀성 화합물과 추출 수율은 medilite 325 mesh, 600 mesh, DW 추출물 순으로 나타났다. DPPH법에 의한 0.05%와 0.1%의 녹차 추출물에서 항산화 활성은 60.95% ∼ 64.15% 사이였다. 간 homogenate 및 microsone 생체막 지질 과산화물 생성 억제 정도는 medilite 325 mesh 및 600 mesh 침출 녹차 추출물에 비해 증류수 침출 녹차 추출물에서 현저히 억제되었다. 특히 Fe 함량은 증류수 침출 녹차 추출물에 비해 medilite 325 mesh 및 600 mesh 침출 녹차 추출물에 현저히 높았다 이러한 medilite 침출 녹차 추출물에서 높은 Fe성분 함량은 microsone 생체막 지질 과산화물의 생성 촉진과 관련된 것으로 사료된다. 이상의 결과에서 녹차 추출물 중의 총 폴리페놀성 화합물, 추출 수율 및 미네랄 성분은 증류수에 비해 medilite 침출수를 이용함으로써 대체적으로 증가하는 결과를 얻었다.

The chemical compositions and antioxidant activity of green tea extracts in medilite-extraction water were compared to that of distilled water(DW). Antioxidant activity was fetermined by the formation of thiobarbituric acid reactive substances (TBARS) in rat liver homogenates and microsomes and the scavenging activity of free radicals by DPPH ($\alpha$, $\alpha$'-diphenyl-$\beta$-picrylhydrazyl). The order of total polyphenolic compounds and extracted yield by extracts was medilite 325 mesh-extraction water, medilite 600 mesh-extraction water and distilled water(DW). The ranges of scavenging activity of green tea extracts in DPPH method were 60.95% - 64.51%. The inhibition ratios of TBARS formation in the rat liver homogenates and microsomal fractions were significantly lower with green tea extracts by DW-extraction than with both medilite 325 mesh and 600 mesh-extraction water. The concentration of iron ion of water containing medilite 325 mesh and green tea extracts and of water containing medilite 600 mesh and green tea extracts were significantly higher compared to DW. Therefore, this result suggested that enhanced concentration of iron ion in green tea extracts by medilite-extraction water containing high iron ion content was associated with enhanced peroxidation of the rat liver microsomal fractions. These results showed that total polyphenolic compounds, the % of yield and mineral compounds of green tea extracts were increased using medilite 325 mesh and 600 mesh-extraction water.

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참고문헌

  1. Official methods of analysis(12th ed.) A.O.A.C.
  2. Nature v.26 Antioxidant determination by the use of a stable free radical Blois,M.S.
  3. J. Nutr. v.122 Iron absorption from bread in humans: inhibiting effects of cereal fiber, phytate and inositol phosphates with different numbers of phosphate groups Brune,M.;L.Rossander-Hulten;L.Hallberg;A.Gleerup;A.S.Sandberg
  4. Korean J. Life Sci. v.10 Effect of Quartz porphyry supplemented diet on mineral content in tissues of common finch Cha,J.Y.;Y.S.Cho;J.B.Ihm;D.J.Kim
  5. J. Korean Soc. Food Sci. Nutr. v.28 Effect of potato polyphenolics on lipid peroxidation in rats Cha,J.Y.;Y.S.Cho
  6. J. Korean Soc. Food Sci. Nutr. v.29 Effect of water-soluble extract from leaves of Morus alba and Cudrania tricuspidata on the lipid peroxidation in tissues of rats Cha,J.Y.;H.J.Kim;Y.S.Cho
  7. J. Korean Soc. Food Sci. Nutr. v.28 Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata Cha,J.Y.;H.J.Kim;C.H.Chung;Y.S.Cho
  8. J. Jpn Soc. Nutr. Food Sci. v.51 Time course of change in hepatic lipid peroxide level in iron-deficient rats Chiba,H.;M.Takasaki;R.Masuyama;M.Uehara;Y.Kanke;K.Suzuki;S.Goto https://doi.org/10.4327/jsnfs.51.201
  9. Korean J. Life Sci. v.10 Changes in chemical compositions of green tea under the different extraction conditions Choi,H.J.;W.S.Lee;S.J.Hwang;I.J.Lee;D.H.Shin;H.Y.Kim;K.U.Kim
  10. Korean J. Food Sci. Technol. v.26 Changes in hydrogen donating activities of the extract from Holoeion maximowiczii root by drying methods Choi,D.Y.;J.H.Do;K.S.Lee;C.B.Yang
  11. Korean J. Food Sci. Technol. v.26 Changes of chemical components in persimmon leaves during growth for processing persimmon leaves tea Chung,S.H.;K.D.Moon;J.K.Kim;J.H.Seong;T.H.Sohon
  12. Food Chem. v.60 Comparison of the antioxidant activity of rooibos tea with green, oolong and black tea Gadow,A.V.;E.Joubert;C.F.Hansmann https://doi.org/10.1016/S0308-8146(96)00312-3
  13. Ann. Pharm Fr. v.38 Pharmaceutical use of some activated bentonites(author's transl) Grecu,I.S.Barbu;L.Ghizdavu
  14. Free radicals, aging and degenerative disease Johnson,J.E.;R.Walford;D.Harma;J.Miquel
  15. J. Japan Soc. Nutr. Food Sci. v.52 Aritioxidant activity of several commercial teas and their components Kajimoto,G.;C.Mutakami https://doi.org/10.4327/jsnfs.52.209
  16. Mar. Pollut Bull v.45 Nutrients adsorption from seawater by new porous carrier made from zeolitized fly ash and slag Khelifi,O.;Y.Kozuki;H.Murakami;K.Kurata;M.Nishioka https://doi.org/10.1016/S0025-326X(02)00107-8
  17. Korean J. Food Sci. Technol. v.26 Analysis of phenolic substances content on Korea plant foods Lee,J.H.;S.R.Lee
  18. J. Jpn Soc. Nutr. Food Sci. v.51 Lipid peroxides and their relatives in organs of female rats fed diets containing excessive heme iron Murakami,A.;M.Kishimoto;M.Kawaguchi;T.Matsuura;T.Ichikawa https://doi.org/10.4327/jsnfs.51.9
  19. J. Nutr. Sci. Vitaminol. v.32 Effect of green tea catechin on plasma cholesterol level in cholesterol fed rats Muramatsu;K.M.Fukuyo;Y.Hara https://doi.org/10.3177/jnsv.32.613
  20. J. Assoc. Off. Anal. Chem. v.64 Effect of bentonites on fluorometric selenium determination Olson,O.E.;A.A.Herr;I.S.Palmer
  21. Annu. Rev. Pharmacol. Toxical. v.16 Chemicals, drugs and lipid peroxidation Plaa,G.L.;H.Witschi https://doi.org/10.1146/annurev.pa.16.040176.001013
  22. Am. J. Clin. Nutr. v.73 Green tea or rosemary extract added to foods reduces nonheme-iron absorption Samman,S.;B.Sandstrom;M.B.Toft;K.Bukhave;M.Jensen;S.S.Sorensen;M.Hansen
  23. J. Pharm. Pharmacol. v.42 Lipid peroxidation effects of a novel iron compound, ferric maltol;A comparison with ferrous sulphate Singh,R.K.;M.A.Barrand https://doi.org/10.1111/j.2042-7158.1990.tb05407.x
  24. J. Sci. Food Agric. v.10 Phenolic constituents of Ptunus domestica. I. Quantitative analysis of phenolic constituents Swain,T.;W.E.Hillis;M.Oritega
  25. Nutr. Cancer v.41 Green tea catechins and vitamin E inhibit angiogenesis of human microvascular endothelial cells through suppression of IL-8 production Tang,F.Y.;M.Meydani https://doi.org/10.1207/S15327914NC41-1&2_17
  26. J. Nutr. Food v.5 Effects of green tea supplemented with indigestible dextrin on postprandial levels of blood glucose and insuline in human subjects Unno,T.;K.Nagata;T.Horiguchi
  27. Poultry Sci. v.70 Effect of dietary zeolite A and graded levels of calcium on growth, plasma, and tibia characteristics of chicks Watkins,L.;L.L.Southern https://doi.org/10.3382/ps.0702295
  28. J. Inorganic Biochem. v.14 The role of superoxide and hydroxyl radical in the degradation of hyaluronic acid induced by metal ions and by ascorbic acid Wong,S.F.;B.Holliwell;R.Richimond;W.R.Skowroneck https://doi.org/10.1016/S0162-0134(00)80033-1

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