Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Molecular probe for identification of cysts of resting cyst of PSP-producer Alexandrium tamarense (Dinophyceae)

분자생물학적 방법을 이용하여 마비성 패류 독소를 생산하는 알렉산드륨 타마렌스 시스트 탐색

  • Cho, Eun-Seob (Harmful Algal Research Division, Author for reprint requests, National Fisheries Research and Development Institute)
  • Published : 2003.04.01
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Abstract

Identification of species within the toxin-producing genus Alexandrium is vital for biotoxin monitoring and mitigation decisions regarding shellfish industry. In particular, the discrimination of resting cysts of only A. tamarense from that of Alexandrium spp. is considerable important to fundamentally monitor and predict this species before vegetative cells occur in the nature. Fluorescent cTAM-F1 DNA probe was responsible to not only binding the activity of the vegetative cells in A. tamarense, but also to the resting cysts, which was treated with methanol after fixation and stained by primuline on the surface The location of fluorescence in cultured vegetative cells and resting cysts was almost at tile bottom of the nucleus. The optimal incubation temperature and time using in situ hybridization were 50-$54^{\circ}C$ and 40-60 min, respectively, to penetrate the DNA probe into cell.

알렉산드륨 적조생물 속에서 마비성 패류독소를 생산하는 종을 신속하게 동정하므로 패류양식의 독성 모니터링과 방제에 중요한 역할을 할 수 있다. 자연상태에서 영양세포가 출현하기 전 알렉산드륨 타마렌스의 휴면포자만을 신속하게 분리 동정한다는 것은 근본적인 마비성 패류독소 모니터링 및 예측에 큰 역할을 할 수 있다. cTAM-Fl DNA probe은 알렉산드륨 타마렌스의 영양세포 뿐만 아니라 primuline으로 염색하여 메타놀로 고정한 휴면포자에도 반응이 되었다. 영양세포와 휴면포자에 반응되는 DNA probe 위치는 핵내의 말단 부위에 보였다. DNA probe가 세포내로 삽입되는데 가장 적합한 온도와 시간은 50-$54^{\circ}C$, 40-60분이 좋았다.

Keywords

References

  1. J. Phycol. v.30 Restriction fragment length polymorphism of ribosomal DNA in ternal transcribed spacer and 5.8S regions in Japanese Alexandrium species (Dinophyceae) Adachi,M.;Y.Sako;Y.Ishida https://doi.org/10.1111/j.0022-3646.1994.00857.x
  2. J. Phycol. v.32 Identification of the toxic dinoflagellates Alexandrium catenella and A. tamarense (Dinophyceae) using DNA probes and whole-cell hybridization Adachi,M.;Y.Sako;Y.Ishida https://doi.org/10.1111/j.0022-3646.1996.00424.x
  3. Harmful Marine Algal Blooms Identification of harmful algal species using molecular probes an emerging perspective Anderson,D.M.;Lassus,P.(ed.);G.Arzul(ed.);E.Erard(ed.);P.Gentien(ed.);C.Marcaillou(ed.)
  4. J. Phycol. v.14 Potential importance of benthic cysts of Gonyaulax tamarensis and G. excavata in initiating toxic dinoflagellate blooms Anderson,D.M.;D.Wall https://doi.org/10.1111/j.1529-8817.1978.tb02452.x
  5. Toxic Dinoflagellates The genus Alexandrium or Gonyaulax of the tamarensis group Balech,E.;Anderson,D.M.(ed.);A.W.White(ed.);D.G.Baden(ed.)
  6. Limnol. Oceanogr. v.23 Use of fluorescence microscopy for quantifying phytoplankton, especially filamentous blue-green algae Brock,T.D. https://doi.org/10.4319/lo.1978.23.1.0158
  7. J. Kor. Lif. Sci. v.12 Application of species-specific DNA probe to field samples of Alexandrium tamarense (Lebour) Balech Cho,E.S.;H.G.Kim;S.K.Moon;J.D.Lee https://doi.org/10.5352/JLS.2002.12.3.250
  8. Bull. Mar. Sci. v.37 Morphology of Protogonyaulax tamarensis (Lebour) Taylor and Protogonyaulax catenella (Whedon and Kofoid);Taylor from Japanese coastal waters Fukuyo,Y.
  9. Can. J. Micro. v.8 Studies of marine planktonic diatoms 1: Cyclotella nana Hustedt, and Detonula confervacea (Cleve) Gran Guillard,R.R.L.;J.H.Ryther https://doi.org/10.1139/m62-029
  10. Phycologia v.32 A review of harmful algal blooms and their apparent global increase Hallegraeff,G.M. https://doi.org/10.2216/i0031-8884-32-2-79.1
  11. Red Tides: Biology, Environmental Science, and Toxicology Methods for modern dinoflagellate cyst studies Matsuok,K.;Y,Fukuyo;D.M.Anderson;Okaichi,T.(ed.);D.M.Anderson(ed.);T.Nemoto(ed.)
  12. J. Phycol. v.36 On detection of Pseudo-nitzschia (Bacillariophyceae) species using whole cell hybridization: sample fixation and stability Miller,P.;C.A.Scholin https://doi.org/10.1046/j.1529-8817.2000.99041.x
  13. J. Phycol. v.32 Identification and enumeration of cultured and wild Pseudo-nitzschia(Bacillariophyceae) using species-specific LSU rRNA-targeted fluorescent probes and filter-based whole cell hybridization Miller,P.;C.A.Scholin https://doi.org/10.1111/j.0022-3646.1996.00371.x
  14. J. Phycol. v.30 Identification of group-and strain-specific genetic markers for globally distributed Alexandrium (Dinophyceae). Ⅰ. RFLP analysis of SSU rRNA genes Scholin,C.A.;D.M.Anderson https://doi.org/10.1111/j.0022-3646.1994.00744.x
  15. Phycologia v.35 Identification of Pseudonitzschia austalis (Bacillariophyceae) using rRNA-targeted probes in whole cell and sandwich hybridization formats Scholin,C.A.;K.R.Buck;T.Britschgi;G.Cangelosi;E.P.Chavez https://doi.org/10.2216/i0031-8884-35-3-190.1
  16. Phycologia v.34 Molecular evolution of the Alexandrium tamarense species complex (Dinophyceae): dispersal in the North American and West Pacific regions Scholin,C.A.;G.M.Hallegraeff;D.M.Anderson https://doi.org/10.2216/i0031-8884-34-6-472.1
  17. J. Phycol. v.30 Identification of group-and strain-specific genetic markers for globally distributed Alexandrium (Dinophyceae).Ⅱ. Sequence analysis of a fragment of the LSU rRNA gene Scholin,C.A.;M.Herzog;M.Sogin;D.M.Anderson https://doi.org/10.1111/j.0022-3646.1994.00999.x
  18. Limnol. Oceanogr. v.42 Detection and quantification of Pseudonitzschia australis in cultured and natural populations using LSU rRNA-targeted probes Scholin,C.A.;P.E.Miller;K.Buck;F.Chavez;P.Harris;P.Haydock;J.Howard https://doi.org/10.4319/lo.1997.42.5_part_2.1265
  19. Rev. Fish. Sci. v.1 The impact of toxic algae on scallop culture and fisheries Shumway,S.E.;A.D.Cembella https://doi.org/10.1080/10641269309388538
  20. Protoplasma v.59 Starch-primulin fluorescence Sterling,C. https://doi.org/10.1007/BF01247861
  21. Seafood Toxins;American Chemical Society Symposium, Series no.262 Toxic dinoflagellates: taxonomic and biogeographic aspects with emphasis on Protogonyaulax Taylor,F.J.R.;Ragelis,E.P.(ed.)
  22. Phycologia v.34 A rapid and precise technique for enumeration of resting cysts of Alexandrium spp. (Dinophyceae) in natural sediments Yamaguchi,Y.;S.Itakura;I.Imai;Y.Ishida https://doi.org/10.2216/i0031-8884-34-3-207.1