Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Drug-Induced Haploinsufficiency of Fission Yeast Provides a Powerful Tool for Identification of Drug Targets

  • PARK, JO-YOUNG (Division of Genome Research, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • YOUNG-JOO JANG (Division of Genome Research, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • SEOG-JONG YOU (DNA Chip Team, Bioneer Corp.) ;
  • YOUNG-SOOK KIL (Division of Genome Research, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • EUN-JUNG KANG (Division of Genome Research, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • JEE-HEE AHN (Division of Genome Research, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • YOUNG-KWON RYOO (Division of Genome Research, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • MIN-YOUN LEE (Division of Genome Research, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • MISUN WON (Division of Genome Research, Korea Research Institute of Bioscience and Biotechnology(KRIBB))
  • Published : 2003.04.01
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Abstract

Genome-wide systematic deletion mutants were generated using a PCR-based targeted mutagenesis of Schizosacchaaromyces pombe. In a drug-sensitivity assay using thiabendazole(TBZ), an inhibitor of microtubule assembly, a heterozygous nda2 mutant ($nda2^+/nda2^-$), deleting one copy of nda2 encoding the microtubule subunit alpha1 demonstrated a distinct sensitivity to TBZ, indicating TBZ-induced haploinsufficiency. This result suggests that profiling drug-induced haploinsufficiency can be exploited to identify target genes for drugs and discover new drugs.

Keywords

References

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