Journal of Plant Biotechnology

  • 제30권1호
  • /
  • Pages.19-25
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  • 2003
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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방사선 이용 벼 돌연변이 계통 선발 및 농경 형질조사

Selection and Agronomic Traits of Radiation-induced Variants in Rice

  • 발행 : 2003.03.01
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초록

본 실험은 방사선 처리에서 유기된 벼 변이계통의 몇 가지 농경 형질 분석 및 RAPD 분석을 통하여 방사선 이용 돌연변이 육종연구를 위한 기초적인 자료를 얻기 위하여 실시하였다. 1. 방사선에 의해 선발된 변이 계통의 초장, 수장 및 내도복성 형질은 모품종과 비교하여 정 (＋)및 부 (－)의 방향으로 작용하였다. 2. RAPD 분석에 의해 계통 간 polymorphic band를 관찰할 수 있었고, UPGMA에 의해 변이계통를 4 groups으로 분류할 수 있었다. 3. 변이체들 중 계통 91, 139, 140 및 141은 내염성 형질을 나타냈고, 유리 proline 함량이 모품종보다 유의성 있는 증가를 나타내었다. 4. 139, 140 및 141 계통은 dendrogram에서 같은 그룹을 이루었고 모품종과 가장 먼 유전적 거리를 나타내었다. 5. 이러한 계통들은 육종 및 분자유전 연구에 유용한 재료로 이용될 수 있다.

Radiation technique in agriculture was initiated to develop mutant rice. Seeds of Daechungbyeo rice were irradiated with 250 Gy gamma ray for the purpose of inducing and selecting rice variants. Some quantitative traits of the variants in M$_{8}$ generation were evaluated and RAPD analysis was carried out. Variants showed a wider range of agronomic characteristics in both a positive and a negative direction compared with their original variety. The new mutants were characterized by an increased or decreased in plant height, lodging resistance and shorter panicle. RAPD analysis showed that polymorphic bands were presented in most of the primers. In comparison with the original variety, variants were classified into four groups through UPGMA analysis. Among mutants no. 91, 139, 140 and 141 was ranked as salt tolerance and the proline content of these mutants was more increased than that of original variety. The lines of 139, 140 and 141 had the highest genetic distance as compared to original variety in the dendrogram. It is expected that such variants will be useful not only for studying molecular genetics but also for breeding research and genetic analysis.s.

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피인용 문헌

  1. Genetic Variation Analysis of Early-heading Plant (Oryza sativa L.) Lines Derived from Gamma-ray Irradiation vol.25, pp.1, 2012, https://doi.org/10.7732/kjpr.2012.25.1.142