International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Molecular Cloning and Expression of a cDNA Encoding Putative Chemosensory Protein from the Mole Cricket, Gryllotalpa orientalis

  • Kim, Iksoo (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology) ;
  • Lee, Kwang-Sik (College of Natural Resources and Life Science, Dong-A University) ;
  • Ryu, Kang-Sun (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology) ;
  • Kim, Jin-Woo (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology) ;
  • Ahn, Mi-Young (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology) ;
  • Lee, Heui-Sam (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology) ;
  • Sohn, Hung-Dea (College of Natural Resources and Life Science, Dong-A University) ;
  • Jin, Byung-Rae (College of Natural Resources and Life Science, Dong-A University)
  • Published : 2003.03.01
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Abstract

We describe here the cloning, expression and characterization of a cDNA encoding a putative chemosensory protein (CSP) from the mole cricket, Gryllotalpa orientalis. The G. orientalis chemosensory protein cDNA sequences comprised of 384 bp with 128 amino acid residues. The G. orientalis chemosensory protein showed 75.4% protein sequence identity to the Locusta migratoria CSP, Northern blot analysis revealed that signal was stronger in head than leg and cuticle, indicating that the head part containing antennae is a main site for G. orientalis chemosensory protein synthesis. The cDNA encoding G. orientalis chemosensory protein was expressed as approximately 12 kDa polypeptide in baculovirus-infected insect cells.

Keywords

References

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