Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Energy Status of Neurospora crassa Mutant nap in Relation to Accumulation of Carotenoids

  • Belozersk, Tatyana A. (Institute of Biochemistry, Russian Academy of Science) ;
  • Potapova, Tatyana V. (A.N.Belozersky Bach Institute of Physico-Chemical Biology, Moscow State University) ;
  • Isakova, Elena P. (Institute of Biochemistry, Russian Academy of Science) ;
  • Shurubor, Eugene I. (Institute of Biochemistry, Russian Academy of Science) ;
  • Savel'eva, Ludmila V. (Institute of Biochemistry, Russian Academy of Science) ;
  • Zvyagilskaya, Renata A. (Institute of Biochemistry, Russian Academy of Science)
  • Published : 2003.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

N crassa mutant strain nap showed reduced growth rate, decreased electric membrane potential, and elevated intracellular ATP content in comparison to the wild type. Blue light induced a hyperpolarization of the membrane potential in both strains. The analysis of oxidative and phosphorylation activities of mitochondria isolated from the two strains has revealed that nap utilized more efficient oxidative pathways. The higher intracellular ATP content in the nap was presumably due to impaired transport systems of the plasma membrane, and to a lesser extent to the functioning of the fully competent respiratory chain. The excess ATP possibly accounts for carotenoid accumulation in the mutant.

Keywords

References

  1. J. Biol. Chem. v.273 Characterization of a high capacity calcium transport system in mitochondria of the yeast Endomyces magnusii Bazhenova, E.N.;Yu.I. Deryabina;R.A. Zvyagilskaya;O. Eriksson;N-E. Saris https://doi.org/10.1074/jbc.273.8.4372
  2. Biol. Membr.(Russian) v.5 Connection of photo-induced hyperpolarization of the Neurospora crassa plasma membrane with the cell metabolic processes Belozerskaya, T.A.;M.S. Kritsky;N.N. Levina;T.V. Potapova;I.S. Soboleva;L.M. Chailakhian
  3. Dokl. Acad. Nauk RAN (Moscow) v.359 Activation of biosynthesis of carotenoids upon genetic ipairement of the plasma membrane Belozerskaya, T.A.;Yu.V. Ershov;N.N.Petrova;A.A. Dmitrovsky;M.S. Kritsky
  4. J. Biol. Chem. v.258 Isolation and characterization of plasma membranes from strains of Neuro-spora crassa with wild type morphology Brooks, K.;R. Addison;G. Scarborough
  5. Adv. Enzymol. v.17 The respiratory chain and oxidative phosphorylation Chance, V.;G.R. Williams
  6. Appl. Biochem. Microbiol. v.16 Effect of dark cultivation temperature on $NADH_2$ photooxidation in Neurospora crassa Chernisheva, E.K.;I.S. Soboleva;M.S. Kritsky
  7. Biochim. Biophys. Acta v.323 A new gene which affects uptake of neutral and acidic amino acids in Neurospora crassa Jacobson, E.C.;R.L. Metzenberg
  8. J. Biol. Chem. v.247 Oxidative phosphorylation in Neurospora mitochondria Lambowitz, A.M.;E.W. Smith;C.W. Slayman
  9. Plant Cell Environ. v.50 The physiological significance of cyanide resistant respiration in higher plants Lambers, H.
  10. Fungal Genet. Biol. v.22 Blue light regulation in Neurospora crassa Linden, H.;P. Ballario;G. Macino https://doi.org/10.1006/fgbi.1997.1013
  11. Arch. Microbiol. v.137 Investigation of electrophysiological responses of Neurospora crassa to blue light Potapova, T.V.;T.A. Belozerskaya;N.N. Levina;M.S. Kritsky;L.M. Chailakhian https://doi.org/10.1007/BF00414555
  12. Biochim. Biophys. Acta v.323 Characteristics of the transport-deficient mutant of Neurospora crassa Rao, T.K.;A.G. DeBask https://doi.org/10.1016/0005-2736(73)90170-3
  13. Oecologia v.95 Water stress, temperature and light effects on the capacity for isoprene emission and photo-synthesis of kudzu leaves Sharkley, T.D.;F. Loretto https://doi.org/10.1007/BF00320984
  14. J. Bacteriol. v.114 Adenine nucleotide levels in Neurospora, as influenced by conditions of growth and by metabolic inhibitors Slayman, C.L.
  15. Uspehi biologicheskoi himii(in Russian) v.40 Action of stress agents on differential gene expression in the course of Neurospora crassa development Sokolovsky, V.Yu.;T.A. Belozerskaya
  16. Appl. Biochem. Microbiol. v.23 Bioluminiscent methods of analysis in microbology Ugarova, N.N.;L.Yu. Brovko;I.Yu. Tradatian;E.I. Rainina
  17. J. Plant Physiol. v.136 Blue light-induced changes in membrane potential and intracellular pH of Phycomyces hyphae Weiss, J.;M.H. Weisenseel https://doi.org/10.1016/S0176-1617(11)81619-6
  18. Z. Naturforsch v.52c Is isoprene emitted by plants synthesized via the novel isoprenyl pyrophosphate pathway? Zeidler, J.G.;H.K. Lichtenthaler;H.U. May;F.W. Lichtenthaler