Improvement of the Stability of the Multibead Assay for Pneumococci by the Use of 6B-protein Complex

피막 다당류 6B-단백질 복합체를 이용한 폐렴구균에 대한 Multibead Assay의 안정성 향상

  • 김지혜 (전북대학교 기초과학연구소) ;
  • 임낙룡 (전북대학교 사범대학 과학교육학부) ;
  • 박문국 (전북대학교 자연과학대학 생물과학부)
  • Published : 2003.03.01

Abstract

A procedure to increase the stability of 6B capsular polysaccharide on microbead surface in the mutibead assay, a serotyping method for Streptococcus pneumoniae, was studied. Pneumococcal capsular polysaccharide 6B was conjugated to bvine serum albumin (BSA), and the coating efficiency and the stability of the 6B-BSA complex was measured. The 6B-BSA complex showed about 200-fold higher coating efficiency to polystyrene surface than 6B polysaccharide. And the stability of the 6B- BSA to be used in the multibead assay for 30 days after coating.

폐렴구균의 혈청형 판별법인 multibead assay에서 사용하는 미세구슬 표면에 코팅한 다당류의 안정성을 높이기 위한 방안을 연구하였다. 폐렴구균 피막 다당류 6B에 bovine serum albumin (BSA)을 결합시킨 다당류-단백질 복합체로 코팅했을 경우와 기존의 방법인 다당류만으로 코팅했을 경우의 코팅 효율과 미세구슬표면에서의 6B 안정성을 비교하였다. 다당류 6B-BSA 복합체를 사용했을 경우에 코팅 효율은 약 200 배 증가하였으며, 미세구슬 표면에서의 6B 안정성도 증가하여, 한번 코팅 후 미세구슬을 사용할 수 있는 기간이 3 일에서 30 일 이상으로 연장될 수 있음을 확인하였다.

Keywords

References

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