Development and Morphology of pelvetia siliquosa Tseng et Chang (Phaeophyta) In Culture

  • Published : 2003.02.01

Abstract

The present study reports morphology and developmental pattern off siliquosa cultured in a laboratory condition. The zygote was spherical with a diameter of 85 ${\mu}{\textrm}{m}$. During development the polarized zygote divided horizontally and the lower daughter cell divided horizontally into 2 cells. The upper cell was divided repeatedly in horizontal and vertical directions to form a cylinder-like structure, which subsequently developed into secondary and tertiary dichotomous branches. Optimum temperature for zygote release and fertilization was 25C. Injury inflicted by slicing was cured by epidermal differentiation, and adventitious branches; the branches emerging from the pith cells, however, developed no rhizoid. Adventitious branch formation rate was over 88% in all plates supplemented with 0.5 mg/L IAA and peaked at 98% under 0.5 mg/L IAA plus 0.5-5.0 mg/L NAA treatment. NAA stimulated the differentiation of adventitious branches at a wide range of concentrations, while IAA, 2,4-D and kinetin exhibited dose-dependent stimulation.

Keywords

References

  1. Bot. Mag. v.82 Rhizoid differentiation under low temperature condition in Fucus eggs. Abe, M.
  2. Bot. Mag. v.83 A method of inducing egg liberation in Fucus evanescens. Abe, M.
  3. New Plant Physiology Cha, J. H.;J. K. Kim;J. S. Maeng
  4. Amer. J. Bot. v.24 The presence of growth regulators during the early development of Fucus. Du Buy, H. G.;R. A. Olson
  5. Ag. Fac. Sc., Hokkaido Univ., Ser. v.5 no.1 Embryological studies on Pelvetia wrightii Yendo and Fucus evanesens. Inoh, S.
  6. L. Biol. Plant. v.22 Cytokinin-like activity in sea water and Fucus vesiculosus. Kentzer, T.;R. Synak;J. Burkiewicz;A. Banas
  7. New Phytol. v.66 The apical meristem of Fucus. Moss, B. L.
  8. Br. Phycologia. Bull. v.3 The culture of fertile tissue of Fucus vediculosus. Moss, B. L.
  9. Br. Phycologia Bull. v.3 The transition from vegetative to fertile tissue in Fucus vesiculosus. Moss, B. L.
  10. Jap. J. Phycologia v.17 no.1 Notes on Fucales (4). A review on the egg polarity. Nakazawa, S.
  11. Phycologia v.6 Formation of a crustaceous sporophyte, with unilocular sporangia in Scyosiphon lomentaria. Tatewaki, M.
  12. Algae v.13 no.2 Developmental morphology on the regeneration of Pelvetia siliquosa Tseng et Chang (Phaeophyta) in Korea. Yoon, J. T.;S. W. Soh.