Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Free Radical Scavenging Effect of Seed and Skin Extracts from Campbell Early Grape (Vitis labruscana B.)

국내산 포도 캠벨종의 종자 및 과피 추출물의 Free Radical 소거능 탐색

  • 박성진 (강원대학교 바이오산업공학부) ;
  • 이현용 (강원대학교 바이오산업공학부) ;
  • 오덕환 (강원대학교 바이오산업공학부)
  • Published : 2003.02.01
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Abstract

This study was conducted to determine optimal extraction condition and free radical scavenging effect of ethanol extracts of Campell Early grape seeds and skins at different temperatures, and of organic solvent fractionated from ethanol extracts of grape seeds and skins. The free radical scavenging effect was tested using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and expressed as dose required for a 50% reduction of DPPH radical (RC$_{50}$). The extraction yields were increased with increasing the extraction temperature but it was not related with ethanol concentrations. The ethanol extract of grape seeds obtained at 5$0^{\circ}C$ showed the more stronger RC$_{50}$ (16.8 $\mu\textrm{g}$/mL) than those of both 3$0^{\circ}C$ and 78$^{\circ}C$. The ethylacetate fraction obtained from ethanol extract of grape seed showed the strongest RC$_{50}$ (15.4 $\mu\textrm{g}$/mL). Overall, both ethanol extracts and their fractions of grape seeds showed significantly stronger free radical scavenging effect than those of skin extracts.racts.

본 연구는 캠벨종 포도의 종자와 과피 에탄올 추출물의 추출조건과 그에 따른 DPPH법에 의한 free radical 소거 활성을 비교하여 최적의 추출 조건의 확립과 항산화 활성을 살펴보았다. 추출 조건은 추출 용매인 에탄올의 농도와 추출 온도를 달리 하여 각각의 수율을 비교하였으며, 각 분획물은 hexane 분획물, chloroform 분획물, ethyl acetate 분획물, butanol 분획물 및 물 분획물로 나누어, free radical 소거 활성을 RC$_{50}$으로 나타내었다. 추출 수율은 종자에서는 추출 온도 78$^{\circ}C$, 에탄올 농도 100%에서 11.4%로 다른 온도와 농도 조건에 비하여 높은 수율을 나타냈으며, 과피 에서도 역시 추출 온도 78$^{\circ}C$, 에탄올 농도 100%에서 44.5%로 다른 온도와 농도 조건에 비하여 높은 수율을 나타냈다. 각 분획물 중에서는 물 분획물이 종자에서는 39.2%인데 비하여 과피 에서는 85.6%로 다른 분획물에 비하여 많이 차지하였다. DPPH법에 의한free radical 소거 활성에서는 종자의 경우, 5$0^{\circ}C$ 에탄을 추출물이 RC$_{50}$=16.8 $\mu\textrm{g}$/mL로, 분획물에서는 ethyl acetate 분획물이 RC/syb 50/=15.4 $\mu\textrm{g}$/mL로 가장 높은 활성을 나타내었다. 과피의 경우, 78$^{\circ}C$ 에탄올 추출물이 RC$_{50}$=2437.5 $\mu\textrm{g}$/mL로, 분획물에서는 butanol 분획물이 RC$_{50}$=698.4 $\mu\textrm{g}$/mL로 가장 높은 활성을 나타내었다. 이상의 결과를 통하여 추출 온도와 용매 농도에 따른 수율의 차이가 있었으며 free radical 소거 활성에서는 종자 에탄을 추출물이 과피 에탄올 추출물 보다 145배 이상의 현저히 높은 활성을 나타내었다.을 나타내었다.

Keywords

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