농약과학회지 (The Korean Journal of Pesticide Science)

  • 제7권3호
  • /
  • Pages.198-206
  • /
  • 2003
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  • 1226-6183(pISSN)
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  • 2287-2051(eISSN)
한국농약과학회 (The Korean Society of Pesticide Science)
권호 표지

Benzimidazole계 살균제의 세포독성 평가

Risk assessment on cytotoxicity for benzimidazole fungicides

  • 이제봉 (농업과학기술원 농약안전성과) ;
  • 성필남 (제주농업시험장 축산과) ;
  • 정미혜 (농업과학기술원 농약안전성과) ;
  • 신진섭 (농업과학기술원 농약안전성과) ;
  • 강규영 (경상대학교 농업생명과학대학 환경생명화학과)
  • 발행 : 2003.09.30
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초록

Benzimidazole계 살균제의 세포독성을 평가하기 위하여 CHL(chinese hamster lung) 세포를 이용한 Giemsa stain assay, Lactic dehydrogenase(LDH) activity, MTT assay, DNA synthesis inhibition test 및 Sulforhodamin B(SRB) assay를 수행하여 benzimidazole 계 살균제에 대한 약제별 세포독성을 평가하고 위해도 평가를 위한 자료로 사용하고자 하였다. 시험결과 LDH 활성은 carbendazim 4.0, 16.0 및 $32.0{\mu}g/mL$을 24 시간 처리하였을 때 음성대조군에 비해 농도별로 세포독성이 각각 2.16, 2.94 및 2.64배 강하게 나타났다. DNA 합성시험에서는 carbendazim $2.0{\mu}g/mL$ 농도에서 약 45% DNA 합성을 저해하였으며, Giemsa assay와 MTT assay에서도 세포와 mitochondria에 독성을 일으키는 것으로 나타났다. Giemsa assay의 $IC_{50}$은 thiophanate-methyl, benomyl, carbendazim 및 양성대조 약제인 captafol에서 각각 >125, 1.2, 30.0 및 $0.3{\mu}g/mL$ 이었고, MTT assay의 $IC_{50}$은 thiophanate-methyl, benomyl, carbendazim 및 captafol에서 각각 >125, 18.7, 20.4 및 $2.6{\mu}g/mL$이었다. 그리고 세포 증식에 대한 영향을 조사하기 위하여 수행된 SRB assay 의 $IP_{50}$(반수세포증 식억제농도)에서도 thiophanate-methyl, carbendazim, benomyl 및 captafol이 각각 17.4, 5.3, 1.5, 및 $0.5{\mu}g/mL$으로 관찰되었다. 이상의 결과는 급성독성이 낮은 benzimidazole계 살균제가 유전독성 등 특수독성이 강하게 나타나는 원인일 것으로 판단되었다.

To assess potential risk of the benzimidazole fungicides, their cytotoxicities were evaluated. Activities of LDH(Lactic dehydrogenase) in the culture fluid of CHL(chinese hamster lung) fiberoblast cell treated with 4.0, 16.0 or $32.0{\mu}g/mL$ of carbendazim for 24 hours were elevated 2.16, 2.94 and 2.64 folds compared to the control, respectively. DNA synthesis was inhibited by 45% at $2.0{\mu}g/mL$ of carbendazim. Benzimidazole fungicides showed high toxicity to cell and mitochondria of CHL cell by Giemsa and MTT (3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide) assay. $IC_{50}$ by the Giemsa assay of thiophanate-methyl, benomyl, carbendazim and captafol were over 125, 1.2, 30.0 and $0.3{\mu}g/mL$, respectively. $IC_{50}$ by the MTT assay of thiophanate-methyl, benomyl, carbendazim and captafol were over 125, 18.7, 20.4 and $2.6{\mu}g/mL$, respectively. Inhibitory concentration of cell median proliferation by SRB (sulforhodamin B) assay for thiophanate-methyl, carbendazim, benomyl, and captafol were 17.4, 5.3, 1.5 and $0.5{\mu}g/mL$, respectively. Accordingly, benzimidazole fungicides inhibited DNA synthesis, mitochondrial function, cell proliferation and induced cell necrosis.

키워드

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