Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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A New Device for Intrauterine Artificial Insemination in the Dog

  • Kong, I.K. (Department of Animal Science and Technology, College of Agriculture and Life Sciences, Sunchon National University) ;
  • Yu, D.J. (Department of Animal Science and Technology, College of Agriculture and Life Sciences, Sunchon National University) ;
  • Jeong, S.R. (Department of Animal Science and Technology, College of Agriculture and Life Sciences, Sunchon National University) ;
  • Oh, I.S. (Department of Animal Science and Technology, College of Agriculture and Life Sciences, Sunchon National University) ;
  • Yang, C.J. (Department of Animal Science and Technology, College of Agriculture and Life Sciences, Sunchon National University) ;
  • Cho, S.G. (Department of Animal Science and Technology, College of Agriculture and Life Sciences, Sunchon National University) ;
  • Bae, I.H. (Department of Animal Science and Technology, College of Agriculture and Life Sciences, Sunchon National University) ;
  • Oh, D.H. (Department of Animal Science and Technology, College of Agriculture and Life Sciences, Sunchon National University) ;
  • Kim, H.R. (Livestock Improvement Main Center, NACF) ;
  • Cho, S.K. (Department of Animal Science, Gyeongsang National University) ;
  • Park, C.S. (Department of Animal Science, Gyeongsang National University)
  • Received : 2002.01.17
  • Accepted : 2002.10.01
  • Published : 2003.02.01
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Abstract

The intrauterine inseminator (IUI) was developed to provide the method of depositing dog semen into the uterine body instead of the vagina. The IUI consists of a vaginal endoscope, a balloon sheath, and injection catheter. When the endoscope is inserted into the vagina and the balloon expanded with air, the cervical os becomes visible so a injection catheter can be inserted through the cervix for deposition of the frozen-thawed semen. The efficacy of the IUI device was compared to intra-vaginal artificial insemination using semen that had been collected and frozen from pooled sperm-rich fraction of ejaculates collected from two Jindo dog donors. Aliquots of semen were extended with a Tris-egg yolk diluent, centrifuged, the seminal plasma removed, the pellet resuspended with the same diluent, and cooled to $5^{\circ}C$ over a 2 h period. A Tris-egg yolk-glycerol extender was added at $5^{\circ}C$; after 1 h, semen was loaded into 0.5 ml straws, and straws were frozen in LN vapor for 5 min, and immersed in LN for storage. The final sperm concentration for freezing was approximately $100{\times}10^{6}cells/ml$. The straws were thawed at $70^{\circ}C$ for precisely 6 sec, 1.5 ml Tris-egg yolk buffer at $38^{\circ}C$ added, and the 2 ml of thawed semen was used for a single insemination using the IUI device. Each bitch was inseminated at optimal insemination point, which was estimated by vaginal epithelial cells staining and progesterone concentration analysis. Use of the IUI device resulted in 21 of 26 females giving birth to 89 pups ($4.2{\pm}1.6$ pups per litter), while intra-vaginal AI resulted in 6 of 15 females whelping a total of 17 pups ($2.8{\pm}1.2$ pups per litter). We believe the IUI device is easier to use than previously described devices used for intrauterine insemination. In our experience the expansion of the balloon has a calming effect on the bitch that aids the inseminator. These results indicate that the IUI device was able to provide high fertility with 50 million frozen sperm per insemination and two inseminations.

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References

  1. Anderson, K. 1972. Fertility of frozen dog semen. Acta. Vet. Scand. 13:128-130.
  2. Anderson, K. 1975. Insemination with frozen semen based on a new insemination technique. Zuchthyg. 10:1-4. https://doi.org/10.1111/j.1439-0531.1975.tb00275.x
  3. Concannon, P. W., J. P. McCann and M. Temple. 1989. Biology and endocrinology of ovulation, pregnancy and parturition in the dog. J. Reprod. Fertil. 39(Suppl.):3-25.
  4. Farstad, W. 1984. The correlation between a cycles coefficient based on cytological indices in the vaginal smear and circulating progesterone in oestrous bitches. Zuchthygiene. 19:211-217. https://doi.org/10.1111/j.1439-0531.1984.tb00972.x
  5. Farstad, W. and K. Andersen Berg. 1989. Factors influencing the success rate of artificial insemination with frozen semen in the dog. J. Reprod. Fertil. 39(Suppl.):289-292.
  6. Fontbonne, A. and F. Badinand. 1993. Canine artificial insemination with frozen semen: comparison of intravaginal and intrauterine of semen. J. Reprod. Fertil. 47(Suppl.):325-327.
  7. Fougner, J. A., J. Aamdal and K. Anderson. 1973. Intrauterine insemination with frozen semen in the blue fox. Nord. Vet. Med. 25:144-149.
  8. Govette, G., C. Linde-Forsberg and B. Strom. 1996. A successful concept for freezing of dog semen. 13th Int Cong Anim Reprod (ICAR), 2:p5-8.
  9. Jedruch, J., Z. Gajewski and K. Ratajska-Michalczak. 1989. Uterine motor responses to an ${\alpha}2$-adrenergic agonist medetomidine hydrochloride in the bitches during the end of gestation and the postpartum period. Acta. Vet. Scand. 85:129-134.
  10. Linde-Forsberg, C. 1991. Achieving canine pregnancy by using frozen or chilled extended semen. Vet. Clin. No. Am. Sm. Anim. Prac. 21:467-485.
  11. Linde-Forsberg, C. 1994. Accurate monitoring of the oestrus cycle of the bitch for artificial insemination. Proc. 19th World Congr. of the WSAVA. pp. 601-605.
  12. Linde-Forsberg, C. 2000. Fertility data from 2041 controlled artificial insemination in dogs. Proc. 4th Int. Symp. Canine Feline Reprod., Oslo, p. 120.
  13. Linde-Forsberg, C. 2001. Intrauterine insemination in the dog using the Scandinavian transcervical catheter and a comparison with other methods. In recent advances in Small Animal Reproduction. A1207.0201. Eds, Concannon PW, England GCW and Verstegen J. MACROBUTTON Html Res Anchor www.ivis.org. Inter. Vet. Inf. Ser., Ithaca.
  14. Linde-Forsberg, C. and M. Forsberg. 1989. Fertility in dogs in relation to semen quality and the time and site of insemination with fresh and frozen semen. J. Reprod. Fertil. 39(Suppl.):299-310.
  15. Linde-Forsberg, C. and M. Forsberg. 1993. Results of 527 controlled artificial insemination in dogs. J. Reprod. Fertil. 47(Suppl.):313-323.
  16. Linde-Forsberg, C., B. Strom and G. Govette. 1999. Comparison of fertility data from vaginal vs. intrauterine insemination of frozen-thawed dog semen: a retrospective study. Theriogenology. 52:11-23. https://doi.org/10.1016/S0093-691X(99)00106-5
  17. Mahi-Brown, C. 1991. Fertilization in dogs. In: Dunbar BS, O'Rand MG (eds), A Comparative Overview of Mammalian Fertilization. New York: Plenum Press, pp. 281-296.
  18. Mialot, J-. P., C. Dumon and B. Cassou. 1985. Insemiantion artificielle chez la chienne: Mise en place de semence fraiche avec le pistolet souple 'OSIRIS'. Pratique medicale et chirurgicale de l'animal de compagnie. 20:213-220.
  19. Pheminster, R. D., P. A. Holst, J. S. Spano and M. L. Hopwood. 1973. Time of ovulation in the Beagle bitch. Biol. Reprod. 8:74-82. https://doi.org/10.1093/biolreprod/8.1.74
  20. Rota, A., B. Strom, C. Linde-Forsberg and H. Rodriguez-Martinez. Effects of equex STM paste on viability of frozen-thawed dog spermatozoa during in vitro incubation at $38^{\circ}C$. Theriogenology, 47:1093-1101. https://doi.org/10.1016/S0093-691X(97)00066-6
  21. Schutte, A. P. 1967. Canine vaginal cytology. II. Cyclic changes. J. Small Anim. Prac. 8:307-311. https://doi.org/10.1111/j.1748-5827.1967.tb04555.x
  22. Silva, L. D. M. and J. P. Verstegen. 1995. Comparisons between three different extenders for canine intrauterine insemination with frozen-thawed spermatozoa. Theriogenology 44:571-579. https://doi.org/10.1016/0093-691X(95)00228-Z
  23. Silva, L. D. M., K. Onclin, F. Snaps and J. Verstegen. 1995. Laparoscopic intrauterine insemination in the bitch. Theriogenology 43:615-623. https://doi.org/10.1016/0093-691X(94)00067-5
  24. Tsutsui, T. 1989. Gamete physiology and timing of ovulation and fertilization in dogs. J. Reprod. Fertil. 39(Suppl.):269-275.
  25. Tsutsui, T., E. Kawakami, I. Murano and A. Ogasa. 1989. Transport of spermatozoa in the reproductive tract of the bitch: Observations through uterine fistula. Jpn. J. Vet. Sci. 51:560-565. https://doi.org/10.1292/jvms1939.51.560
  26. Tsutsui, T, T. Tezuka, T. Shimizu, I. Murao, E. Kawakami and A. Ogasa. 1988. Artificial insemination with fresh semen in Beagle bitches. Jpn. J. Vet. Sci. 50:193-198. https://doi.org/10.1292/jvms1939.50.193
  27. Wildt, D. E., W. B. Chakraborty, W. B. Panko and S. W. J. Seager. 1978. Relationship of reproductive behavior, serum leteinizing hormone and time of ovulation in the bitch. Biol. Reprod. 18:561-570. https://doi.org/10.1095/biolreprod18.4.561
  28. Wilson, M. S. 1993. Nonsurgical intrauterine insemination in bitches using frozen semen. J. Reprod. Fertil. 47(Suppl.):307-311.