DOI QR코드

DOI QR Code

Effect of Gossypol from Cottonseed Meal Diets on Some Clinico-biochemical Parameters and Humoral Immune Response of Crossbred Calves Fed Barley or Sorghum

  • Pattanaik, A.K. (Centre of Advanced Studies in Animal Nutrition, Indian Veterinary Research Institute) ;
  • Sastry, V.R.B. (Centre of Advanced Studies in Animal Nutrition, Indian Veterinary Research Institute) ;
  • Singh, D.K. (Division of Veterinary Public Health, IVRI) ;
  • Goswami, T.K. (Immunology Section, IVRI) ;
  • Mohanty, D.N. (Division of Animal Reproduction, IVRI)
  • 투고 : 2002.07.24
  • 심사 : 2003.05.12
  • 발행 : 2003.09.01

초록

Effects of feeding of 9.95 mg free gossypol/kg live weight through cottonseed meal (CSM) were studied in 20 intact male calves fed barley or sorghum as source of cereal during the experimental duration of 210 days. Serum concentration of total protein, albumin, globulin and their ratio did not vary because of protein (gossypol) or cereal sources. Serum level of cholesterol and urea were lower (p<0.05) in sorghum than barley fed calves. Feeding of gossypol through CSM enhanced (p<0.05) serum cholesterol. An interaction between protein and period was observed with respect to serum concentrations of urea, creatinine and alanine transferase. The levels of serum creatinine and alanine transferase increased (p<0.05) following 120 days of experimental feeding in calves fed CSM diets compared to the control animals fed groundnut meal diets. No effect of feeding gossypol was, however, evident on the serum enzymes viz. alanine and aspartate transferases and alkaline phosphatase. Moreover, the source of cereal and protein did not appear to influence the metabolic profile of the calves. Humoral immune response, measured through antibody titre against Brucella abortus S99 innoculation, revealed a delayed and depressed seroreactivity indicative of immunocompromisation because of the phytotoxin gossypol. In conclusion, the feeding of gossypol at the designated levels, although had no deleterious clinico-biochemical manifestations, affected the humoral immune response of the calves.

키워드

참고문헌

  1. Alton, G. G., L. M. Jones and D. E. Pietz. 1975. Laboratory Techniques in Brucellosis. 2nd ed. WHO Monograph Series 55, WHO, Geneva.
  2. Barraza, M. L., C. E. Coppock, K. N. Brooks, D. L. Wilks, R. G. Saunders and G. W. Latimer, Jr. 1991. Iron sulphate and feed pelleting to detoxify free gossypol in cottonseed diets for dairy cattle. J. Dairy Sci. 74:3457-3467. https://doi.org/10.3168/jds.S0022-0302(91)78536-6
  3. Botsoglou, N. A. and D. C. Kufidis. 1990. Determination of total gossypol in cottonseed and cottonseed meals by derivative uvspectrophotometry. J. Assoc. Off. Anal. Chem. 73:447-451.
  4. Botosglou, N. A. 1991. Determination of free gossypol in cottonseed and cottonseed meals by second derivative ultraviolet spectriphotometry. J. Agric. Food Chem. 39:478-482. https://doi.org/10.1021/jf00003a010
  5. Chase, C. C., P. Bastidas, J. L. Ruttle, C. R. Long and R. D. Randell. 1994. Growth and reproductive development in Brahman bulls fed diets containing gossypol. J. Anim. Sci. 72: 445-452. https://doi.org/10.2527/1994.722445x
  6. Clawson, A. F., F. H. Smith, J. C. Osborne and F. R. Barrick. 1961. Effect of protein source, autoclaving and lysine supplementation on gossypol toxicity. J. Anim. Sci. 20:547-552. https://doi.org/10.2527/jas1961.203547x
  7. Colin-Negrate, J., H. E. Kiesling, T. T. Ross and J. F. Smith. 1996. Effect of whole cottonseed on serum constituents, fragility of erythrocyte cells, and reproduction of growing Holstein heifers. J. Dairy Sci. 79:2016-2023. https://doi.org/10.3168/jds.S0022-0302(96)76574-8
  8. FAO. 1997. Cottonseed production. Food and Agriculture Organization of the United Nations. FAO Production Yearbook. 44:46.
  9. Haschek, W. M., V. R. Beasley, W. B. Buck and J. H. Finnel. 1989. Cottonseed meal toxicosis in a swine herd. J. Am. Vet. Med. Assoc. 195:613-615.
  10. Horn, R. J., S. P. Kolfun, Sr. and G. Abraham. 1987. The potential commercial aspects of gossypol. J. Am. Oil Chem. Soc. 64:1315-1321. https://doi.org/10.1007/BF02540789
  11. Jarquin, R., R. Bressani, L. G. Elias, C. Tajada, M. Gonzalez and J. E. Braham. 1966. Effect of cooking and calcium and iron supplementation on gossypol toxicity in swine. J. Agric. Food Chem. 14:275-279. https://doi.org/10.1021/jf60145a021
  12. Kaneko, J. J., 1980. Clinical Biochemistry of Domestic Animals. 3rd ed. Academic Press Inc. Orlando, Florida.
  13. Kaysen, G. A., S. M. Pond and M. H. Roger. 1987. Combined hepatic and renal injury in animals during therapeutic use of acetaminpophen. Arch. Intl. Med. 145:2019-2023.
  14. Keery, C. M., J. C. Allen and W. A. Nipper. 1991. Effects of ensiling on the digestibility and utilization of whole oilseeds by wethers. J. Dairy Sci. 74:518-525. https://doi.org/10.3168/jds.S0022-0302(91)78199-X
  15. Kerr, L. A., 1989. Gossypol toxicity in cattle. Compend. Ed. Pract. Vet. 11:1139.
  16. Lindsey, T. O., G. E. Hawkins and L. D. Guthrie. 1980. Physiological responses of lactating cows to gossypol from cottonseed meal rations. J. Dairy Sci. 74:518-525. https://doi.org/10.3168/jds.S0022-0302(91)78199-X
  17. Morgan, S. E., 1989. Gossypol as a toxicant in livestock. Vet Clin. N. Am. Food Anim. Pract. 5:251. https://doi.org/10.1016/S0749-0720(15)30975-0
  18. Murray, R. K., D. K. Grannier, P. A. Mayes and W. W. Rodwell. 1993. Plasma proteins, immunoglobins, and blood coagulations. In: Harper's Biochemistry, 23rd ed. Appleton & Lange, Norwalk, CT, p. 665.
  19. Nagalakshmi, D., V. R. B. Sastry, D. K. Agrawal and R. C. Katiyar. 2001. Haematological and immunological response in lambs fed on raw and variously processed cottonseed meal. Asian-Aust. J. Anim. Sci. 14:21-29. https://doi.org/10.5713/ajas.2001.21
  20. Nielsen, K., P. F. Wright, J. Cherwongorodzky, J. R. Duncan and B. Stemshorn. 1987. Enzyme immunology for diagnosis of bovine brucellosis. Ann. Instt. Pasteur, 138:75. https://doi.org/10.1016/0769-2609(87)90078-0
  21. Nikokyris, P., K. Kandylis, K. Deligiannis and D. Liamadis. 1991. Effect of gossypol content of cottonseed cake on blood constituents in growing-fattening lambs. J. Dairy Sci. 74:4305-4313. https://doi.org/10.3168/jds.S0022-0302(91)78625-6
  22. Pattanaik, A. K., V. R. B. Sastry and R. C. Katiyar. 1999. Effect of different degradable protein and starch sources on blood and rumen biochemical profile of early weaned crossbred calves. Asain-Aust. J. Anim. Sci. 12:728-734. https://doi.org/10.5713/ajas.1999.728
  23. Pons, W. A. 1977. Gossypol analysis, past and present. J. Assoc. Off. Anal. Chem. 60:252.
  24. Prasse, K. W. 1986. Clinical Pathology. Proceedings of the Postgraduate Committee in Veterinary Science No. 93. University of Sydney, New Sout Wales, Australia. pp. 33-52.
  25. Reiser, R. and H. C. Fu. 1962. The mechanism of gossypol detoxification by ruminant animals. J. Nutr. 76:215.
  26. Risco, C. A., C. A. Holmberg and A. Kutchas. 1992. Effect of graded concentrations of gossypol on calf performance: toxicological and pathological considerations. J. Dairy Sci. 75:2787-2798. https://doi.org/10.3168/jds.S0022-0302(92)78042-4
  27. Rogozhin, P. S., V. M. Bogdanov and N. A. Belichenko. 1986. Effect of gossypol containing feeds on immunoreactivity in pigs. Trudy Uzbekskogo Nouchnoiss Ledovatelskogo i Veterinarnogo Instituta No. 40: 67-71.
  28. Snedecor, G. W. and W. G. Cochran. 1967. Statistical Methods. 6th ed. Oxford and IBH Publ. Co., New Delhi.
  29. Suryavanshi, S. N., H. G. Bhandarkar and S. S. Bhagwat. 1993. Pathological investigations of acute cottonseed toxicity in rabbit and chicken. Livestock Advisor 18:30-32.
  30. Velasquez-Pereira, J., C. A. Risco, L. R. McDowell, C. R. Staples, D. Prichard, P. J. Chenoweth, F. G. Martin, S. N. Williams, II, L. X. Rojas, M. C. Calhoun and N. S. Wilkinson. 1999. Longterm effects of feeding gossypol and vitamin E to dairy calves. J. Dairy Sci. 82:1240-1251. https://doi.org/10.3168/jds.S0022-0302(99)75347-6
  31. Warren, H. M., S. A. Neutze, J. M. Morrison and P. J. Nichlas. 1988. Whole cottonseed in a wheat-based maintenance ration for sheep. Aus. J. Exp. Agric. 28:453-458. https://doi.org/10.1071/EA9880453
  32. Williams, G. L., A. R. Morgan and M. E. Weherman. 1989. Effects of feeding high lipid supplements via whole cottonseed on reproduction in the cow and heifer. The Texas Agric. Exp. Stn. Info. Rep. No. 89-1, College Station.
  33. Zelski, R. Z., J. T. Rothwell, R. E. Moore and D. J. Kennedy. 1995. Gossypol toxicity in preruminant calves. Aust. Vet. J. 72:394-398. https://doi.org/10.1111/j.1751-0813.1995.tb06180.x

피인용 문헌

  1. Gossypol Toxicity from Cottonseed Products vol.2014, pp.1537-744X, 2014, https://doi.org/10.1155/2014/231635
  2. Effects of gossypol from cottonseed cake on the blood profile in sheep vol.48, pp.5, 2016, https://doi.org/10.1007/s11250-016-1039-0
  3. Gossypol as undesirable substance in animal feed - Scientific Opinion of the Panel on Contaminants in the Food Chain : Gossypol as undesirable substance in animal feed - Scientific Opinion of the Pane vol.7, pp.1, 2003, https://doi.org/10.2903/j.efsa.2009.908