Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지

Comparison of RAPD Profiles and Phenotypical Characters of Streptococcal Strains

연쇄상구균의 표현형적 특성과 RAPD profiles 비교

  • Song, Jin-Gyeong (Department ofAqualife Medicine, Yosu National University) ;
  • Kim, Jong-Hun (Department ofAqualife Medicine, Yosu National University) ;
  • Kim, Eun-Hui (Department ofAqualife Medicine, Yosu National University)
  • 송진경 (여수대학교 수산샹명의학과) ;
  • 김종훈 (여수대학교 수산샹명의학과) ;
  • 김은희 (여수대학교 수산샹명의학과)
  • Published : 20030000
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Abstract

Streptococcal infection is one of the most serious disease of cultured olive flounder, Paralychthys olivaceus in Korea and caused by more than one species. However, there has been considerable confusions about the taxonomic position of the fish pathogenic streptococci. In this study, We performed the randomly amplified polymorphic DNA(RAPD) pattern analysis to evaluate the possible classification in 8 streptococci isolated from diseased olive flounder and reference strains based on their DNA structure. RAPD PCR with DNA solution prepared by simple boiling and 10-mer random primer was appeared to be a good tool for discrimination of different streptococcal strains. Phenotypical characters by simple biological test and API 20 Strep corresponded well to the specific profiles of RAPD in streptococcal isolates of this study. Therefore, the RAPD profile was considered as one of differential characters to discriminate the streptococcal isolates from diseased olive flounder.

Keywords

References

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