한국어병학회지 (Journal of fish pathology)

  • 제16권1호
  • /
  • Pages.61-68
  • /
  • 2003
  • /
  • 1226-0819(pISSN)
  • /
  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
권호 표지

연쇄상구균의 약제내성과 전이성 R-plasmid의 특성

Multiple Drug Resistance and Transferable R-plasmid of Streptococci Isolated from Diseased Olive Flounder

  • 김종훈 (여수대학교 수산생명의학과) ;
  • 임대환 (여수대학교 수산생명의학과) ;
  • 이창훈 (국립수산과학원 자원조성연구소) ;
  • 이수정 (부산지방해양수산청 수산관리과) ;
  • 이월라 (마산지방해양수산청 남해수산기술관리소) ;
  • 황미혜 (목포지방해양수산청 해남수산기술관리소) ;
  • 김은희
  • Kim, Jong-Hun (Department ofAqualifeMedicine, YosuNationalUniversity) ;
  • Im, Dae-Hwan (Department ofAqualifeMedicine, YosuNationalUniversity) ;
  • Lee, Chang-Hun (ResourcesEnhancement Institute, NationalFisheries Research&Development Institute) ;
  • Lee, Su-Jeong (Fisheries Management Division, BusanMaritime Affairs and Fisheries Office) ;
  • Lee, Wol-Ra (Namhae Fisheries Technology Institute, Masan RegionalMaritime affairsand Fisheries Office) ;
  • Hwang, Mi-Hye (Haenam Fisheries Technology Institute, MokpoRegionalMaritime affairsand Fisheries Office,) ;
  • Kim, Eun-Hui
  • 발행 : 20030000
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

MICs of 8 chemotherapeutic agents against forty streptococcal isolates were determined. These strains were isolated from diseased olive flounder, Paralichthys olivaceus, and showed 2-5 multiple drug resistance against different antibacterial agents including ampicillin, doxycycline (DOXY), erythromycin, florfenicol, flumequine, norfloxacin, oxolinic acid, and oxytetracycline (OTC). In conjugation experiment, we found transferable R plasmids carrying OTC and DOXY resistance determinant in 3 drug resistance strains analyzed. Six out of 40 isolates showed positive signal in colony hybridization with the R plasmid DNA (pST9) as a probe. It suggests that other types R plasmid different from pST9 is also involving in multiple drug resistance of streptococci isolated from olive flounder.

키워드

참고문헌

  1. Adams, C.A., Austin, B., Meaden, P.G. and Mcintosh, D. : Molecular characterizationof plasmid mediated oxytetracycline resistance in Aeromonas salmonicida. Appl. Environ. Microbiol.,64 : 4194-4201,1998
  2. Anderson, D.G. and McKay, L.L. : Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl. Environ. Microbiol.,46 : 549-552, 1983
  3. Aoki, T. : Drug resistant plasmids from fish pathogen. Microbiol. Sci., 5: 219-223, 1988
  4. Ausbel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A, and Struhl, K. : Short protocols in molecular biology, 3rd.,John Wiley& Son, 1995
  5. Choi, M.S., Park, K.H., Choi, S.H., Jang, S.I., Yoon,C. Y.,Cho, J. G. and Song, H. J. : Survey of drug resistance in Edwardsiella tarda isolated from diseased eels (Anguilla japonica). J. Fish. Pathol., 9 : 195-210, 1996. (in Korean)
  6. Choi, M.S., Park, K.H., Joung, K.M., Shim, H.B. and Yun, S.H. : Effect of levamisole on immunomodulation of eels (Anguilla japonica) in vitro. J. Fish Pathol., 12: 16-23,1999 (in Korean)
  7. Eldar, A., Horovitcz, A. and Bercovier, H. : Development and efficacy of a vaccine against Streptococcus iniae infection in farmed rainbow trout. Vet. Immunol. Immunopathol.,56: 175-183, 1997 https://doi.org/10.1016/S0165-2427(96)05738-8
  8. Hwang, M.H., Park, S.1. and Kim, ye. : Effect of dietary herb medical stuff on the non-specific immune response of nile tilapia, Oreochromis niloticus. J. Fish Pathol., 12 :5-15,1999. (in Korean)
  9. Kim, E. : Transferable R plasmid of Edwardsiella tarda isolated from diseased flounder, Paralichithys olivaceus. J. Fish Pathol., 12 :115-121,1999. (in Korean)
  10. Kim, E. : Drug sensitivity of Streptococcus sp. isolated from cultured flounders. Bull. Yosu Nat'l Univ., 14: 583-589,1999. (in Korean)
  11. Kim, E.. and Aoki, T. : Drug resistance and broad geographical distribution of identical R plasmid of Pasteurella piscicida isolated from cultured yellowtail in Japan. Microbiol. Immunol., 37: 103-109,1993 https://doi.org/10.1111/j.1348-0421.1993.tb03186.x
  12. Kim, E. and Aoki, T. : The transposon like structure of IS26-tetracycline, and kanamycin resistance determinant derived from transferable R plasmid of fish pathogen, Pasteurella piscicida. Microbiol. Immunol., 37 : 103-109, 1994
  13. Kwon, M.G., Kim, Y.C., Sohn, Y.C. and Park, S.I. : The dietary supplementing effects of kugija, Lycium chinense, on immune responses of nile tilapia, Oreochromis niloticus, to Edwardsiella tarda. 1. Fish Pathol., 12 : 7381,1999. (in Korean)
  14. L'Abee-Lund, T.M. and Sorum, H. : Funtional Tn5393-like transposon in the R plasmid pRAS2 from the fish pathogen Aeromonas salmonicida subspecies salmonicida isolated in Norway. Appl. Environ. Microbiol., 66 : 5533-5535, 2000 https://doi.org/10.1128/AEM.66.12.5533-5535.2000
  15. Lee, D.C., Lee, J.., Park, C.I. and Park, S.1. : The study on the causal agent of streptococcicosis (Lactococcus garvieae), isolated from cultured marine fishes. J. Fish Pathol., 14 : 71-80,2001. (in Korean)
  16. Murray, P.R., Baron, E.J., Pfaller, M.A., Tenover, Ee. and Yolken, R.H. : Manual of clinical microbiology. 7th ed. 1469-1497. American Society for Microbiology, Washinton, USA, 1999
  17. Nguyen, H.T. and Kanel, K. : Selective agars for the isolation of Streptococus iniae from Japanese flounder, Paralichthys olivaceus, and its cultural environment. J. Appl. Microbiol.,86: 769-776,1999 https://doi.org/10.1046/j.1365-2672.1999.00724.x
  18. Oh, S.P., Kim, D.W., Lee, J.J. and Lee, C.H. : Bacterial disease in flounder farms of Cheju island. J. Fish Pathol., 11 : 23-27, 1998. (in Korean)
  19. O'Sullivan, D.J. and Klaenhammer, T.R. : Rapid mini prep isolation of high quality plasmid DNA from Lactococcus and Lactobacillus spp. Appl. Environ. Microbiol., 59 : 2730-2733,1993
  20. Yoo, M.H., Jeong, J.B., Kim, E., Lee, H.H. and Jeong, H.D. : Application of a new conjugation method to fish pathogenic bacteria containing R plasmid for the analysis of drugresistant status in aquaculture. J. Korean Fish. Soc., 35: 115-121,2002. (in Korean)
  21. 심두생, 이주석, 허문수, 김진우, : 양식생물질병진단 연구, 국림수산진흥원 사업보고서, 405-423, 1995
  22. 전홍기, 김선희 : 항생물질과 약제내성 메커니즘. pp.395. 부산대학교, 2000