Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Genetic relationship of the VHSV(Viral Hemorrhagic Septicemia Virus)isolated from cultured olive flounder, Paralichthys olivaceus in Korea

우리나라 양식 넙치, Paralichthys olivaceus에서 분리된 VHSV(Viral Hemorrhagic Septicemia Virus)의 유전학적 검토

  • Kim, Su-Mi (Institute ofFisheries Science, Pukyong National University) ;
  • Lee, Jae-Il (Department ofAquatic Life Medicine, College ofFisheries Science,Pukyong National University) ;
  • Hong, Mi-Ju (Ulsan Regional Maritime Affairs & Fisheries Office) ;
  • Park, Heon-Sik (Ulsan Regional Maritime Affairs & Fisheries Office) ;
  • Park, Su-Il (Department ofAquatic Life Medicine, College ofFisheries Science,Pukyong National University)
  • 김수미 (부경대학교 수산과학연구소) ;
  • 이재일 (부경대학교 수산생명의학과) ;
  • 홍미주 (부경대학교 수산생명의학과) ;
  • 박헌식 (울산지방해양수산청) ;
  • 박수일 (부경대학교 수산생명의학과)
  • Published : 20030000
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Abstract

RT-PCR method was applied to detect and clone the nucleocapsid protein (N) gene and glycoprotein (G) gene for sequencing 5 Korean VHSV isolates from cultured olive flounder, Paralichthys olivaceus. Phylogenetic analysis was performed to investigate their relationship with the VHSV strains described previously and isolated from different geographical area. Generally, VHSV strains were separated phylogenetically according to the major geographical area of isolation: Genogroup I (American type), Genogroup Il (British Isles) and Genogroup ill (European type). This study revealed that all 5 Korean VHSV isolates were belonged to Genogroup I and closely related to Japanese Obama25 type.

Keywords

References

  1. Basurco, B. and Benmansour A. : Distant strains of the fish rhabdovirus VHSV maintain a sixth functional cistron which codes for a nonstructural protein of unknown function. Virology, 212: 741-745,1995 https://doi.org/10.1006/viro.1995.1534
  2. Benmansour, A., Basurco, B., Monnier, A.F., Vende, P., winton, J.R. and Kinkelin, P. :Sequence variation of the glycoprotein geneidentifies three distinct lineages within field isolates of viral haemorrhagic septicaemia virus, a fish rhabdovirus. J. Gen. Virol., 78 :2837-2846, 1997 https://doi.org/10.1099/0022-1317-78-11-2837
  3. Bernard, J., Lecocq-Xhonneux, F., Rossius, M.,Thiry, M.E. and de Kinkelin, P. : Cloning and sequencing the messenger RNA of the N gene of viral haemorrhagic septicaemia virus. J. Gen. Virol., 71(8) : 1669-1674,1990
  4. Betts, A.M. and Stone, D.M. : Nucleotide sequence analysis of the entire coding regions of virulent and avirulent strains of viral haemorrhagic septicaemia virus. Virus Genes, 20(3) 11 : 259-262,2000
  5. Bourhy, H., Kissi, B., Lafon, M., Sacramento, D. and Tordo, N. : Antigenic and molecular characterisation of bat rabies virus in Europe. J. Clin. Microbiol., 30 : 2419-2426, 1992
  6. Bourhy, H., Kissi, B. and Tordo, N. : Molecular diversity of the Lyssavirus genus. Virology, 194: 70-81,1993 https://doi.org/10.1006/viro.1993.1236
  7. Brunson, R., True, K. and Yancey, J. : VHS virus isolated at Makah National Fish Hatchery. Am. Fish. Soc. Fish Health Sec. Newsl., 17 : 3-4,1989
  8. Horlyck, V., Mellergard, S., Dalsgaard, I. and Jorgensen, P.E.V. : Occurrence of VHS in Danish maricultured rainbow trout. Bull. Eur.Assoc. Fish Pathol., 4 : 11-13, 1984
  9. Isshiki, T., Nishizawa, T., Kobayashi, T., Nagano, T. and Miyazaki, T. : An outbreak of VHSV(viral hemorrhagic septicemia virus) infection in farmed Japanese flounder Paralichthys olivaceus in Japan. Dis. Aquat. Org., 47(2) : 87-99,2001 https://doi.org/10.3354/dao047087
  10. Jensen, NJ., Bloch, B. and Larsen, J.L. : The ulcussyndrome in cod (Gadus morhuna) Ⅲ. A preliminary virological report. Nord. Vet. Med., 31: 436-442, 1979
  11. Le Berre, M., de Kinkelin, P. and Metzger, A. :Identification serologique des rhabdovirus des salmonides. Bull. Off. Epizoot., 87 :391-393,1977
  12. Lorenzen, N., Olesen, N. J., Jorgensen, P.E.V., Etzerodt, M., Holtet, T.L. and Thoegersen, H.C. :Characterization of intramolecular disulfide bonds and secondary modifications of the glycoprotein from viral hemorrhagic septicemia virus, a fish rhabdovirus. J. Virol., 72 : 10189-10196, 1998
  13. Meyers, T.R., Sullivan, J., Emmengger, E., Follet, E., Short, S., Batt, WN. and Winton, J.R. :Identification of viral hemorrhagic septicemia virus isolated from Pacific cod Gadus macrocephalus in Prince William Sound, Alaska, USA. Dis. Aquat. Org., 12 : 167-175,1992 https://doi.org/10.3354/dao012167
  14. Meyers, T.R., Short, S., Lipson, K, Batts, WN., Winton, J.R., Wilcock, J. and Brown, E. : Association of viral haemorrhagic septicaemia virus with epizootic haemorrhages of the skin in pacific herring Clupea harengus pallasi from Prince William Sound and Kodiak Island, Alaska USA. Dis. Aquat. Org., 19: 27-37,1994 https://doi.org/10.3354/dao019027
  15. Mortensen, H.F., Heuer, O.E., Lorenzen, N., Otte, L. and Olesen, N.J. : Isolation of viral haemorrhagic septicaemia virus (VHSV) from wild marine fish species in the Baltic Sea, Kattegat, Skagerrak and the North Sea. Virus Res., 63: 95-106,1999 https://doi.org/10.1016/S0168-1702(99)00062-3
  16. Nishizawa, T., lida, H., Takano, R., Isshiki, T., Nakajima, K and Muroga, K : Genetic relateness among Japanese, American and European isolates of viral hemorrhagic septicemia virus(VHSV) based on partial G and P genes. Dis. Aquat. Org., 48 : 143-148, 2002 https://doi.org/10.3354/dao048143
  17. Office International des Epizooties : International Aquatic animal health code and diagnostic manual for aquatic animal disease. OIE, Paris, 2001
  18. Ross, K., McCarthy, U., Huntly, P.J., Wood, B.P., Stuart, D., Rough, E.I., Smail, D.A. and W Bruno, D. : An outbreak of viral haemorrhagic septicaemia (VHS) in turbot (Scophthalmus maximus) in Scotland. Bull. Eur. Assoc. Fish Pathol., 14: 213-214, 1994
  19. Schlotfeldt, H.J., Ahne, W, Jorgensen, P.E.V. and Glende, W. : Occurrence of viral haemorrhagic septicaemia in turbot (Scophthalmus maximus) a natural outbreak. Bull. Eur. Assoc. Fish Pathol., 11 : 105-107,1991
  20. Schutze, H., Mundt, E. and Mettenleiter, T.C. :Complete genomic sequence of viral hemorrhagic septicemia virus, a fish rhabdovirus. Virus Genes, 19 (1): 59-65,1999
  21. Smail, D.A. : Isolation and identification of viral haemorrhagic septicaemia (VHS) virus from North Sea cod (Gadus morhua L.), ICES Mariculture Committee Report CM. 1995/f: 15 : 1-6, 1995
  22. Smail, D.A. : Viral haemorrhagic septicaemia. In: Wood PTK, Bruno DW (eds) Fish disease and disorders, vol 3, Viral, bacterial and fungal infections. CABI Publishing, New York, pp 123-147, 1999
  23. Snow, M., Cunningham, C.O., Melvin, W.T. and Kurath, G. : Analysis of the nucleoprotein gene identifies distinct lineages of viral haemorrhagic septicaemia virus within the European marine environment. Virus Res.,63: 35-44, 1999 https://doi.org/10.1016/S0168-1702(99)00056-8
  24. Stone, D.M., Way, K. and Dixon, P.F. : Nucleotide sequence of the glycoprotein gene of viral haemorrhagic septicaemia (VHS) viruses from different geographical areas: a link between VHS in farmed fish species and viruses isolated from North Sea cod (Gadus morhua L.). J. Gen. Virol., 78 : 1319-1326,1997 https://doi.org/10.1099/0022-1317-78-6-1319
  25. Takano, R., Nishizawa, T., Arimoto, M. and Muroga, K. : Isolation of viral haemorragic septicemia (VHSV) from wild Japanese flounder, Paralichthys olivaceus. Bull. Eur. Assoc. Fish Pathol., 20 : 186-193, 2000
  26. van Regenmortel, M..H.V., Fauquet, C.M., Bishop, D.H.L., Carstens, E.B. and 7 others (eds) :Virus taxonomy: the classification and nomenclature of viruses. The Seventh Report of the International Committee on Taxonomy of Viruses. Academic Press, SanDiego, 2000
  27. Winton, J.R, Bans, W.N., Nishizawa, T. and Stehr, CM. : Characterization of the first North American isolates of viral hemorrhagic septicemia virus. Am. Fish. Soc. Fish Health Sect. Newsl., 17: 2-3,1989