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The Influences of Addition of Sugar with or without L. buchneri on Fermentation and Aerobic Stability of Whole Crop Maize Silage Ensiled in Air-stress Silos

  • Guan, Wu-Tai (Dept. of Animal Nutrition, College of Animal Science, South China Agricultural University) ;
  • Driehuis, F. (Dept. of Animal Nutrition, Institute for Animal Science and Health (ID-DLO)) ;
  • Van Wikselaar, P. (Dept. of Animal Nutrition, Institute for Animal Science and Health (ID-DLO))
  • 투고 : 2002.07.24
  • 심사 : 2003.07.07
  • 발행 : 2003.12.01

초록

The whole plant of crop maize was chopped and ensiled in double-layered polyethylene bags to determine the influence of residual sugar on the fermentation of lactic acid and aerobic stability by L. buchneri in whole crop maize silage made in airstress condition. There were a total of six treatments used in this experiment as follow: added 25 g de-mineralised water per kg chopped maize serving as control (con), 37.5 g glucose solution containing 12.5 g glucose ($g_1$), 75 g glucose solution containing 25 g glucose ($g_2$), 25 g, L,.buchneri suspension intended for $10^6$ cfu $g^{-1}$ (L.b.), $g_1$+L.b. and $g_2$+L.b. All silos were opened at day 91 after ensiling for measuring the pH values, microbiological enumeration, fermentative products and aerobic stability. The dry matter loss increased significantly (p<0.01) due to inclusion of sugar or L. buchneri. The lower lactic acid concentrations were observed (p<0.01) in silages inoculated with L. buchneri only or in combination with sugar addition than the correspondent uninoculated silages. Compared with control silage, ethanol production was about 3 or 6-fold higher due to addition 12.5 or 25 g glucose per kg chopped maize at ensiling. The silages added with sugar contained less acetic acid concentration (p<0.01) than control, but silages inoculated with L. buchneri showed the contrary effects (p<0.01) at different sugar levels. No butyric acid was found in uninoculated silages, silages inoculated with L. buchneri. producted more propionic acid, 1-propanol and butyric acid. Lactic acid bacteria counts increased markedly (p<0.01) due to inoculation with L. buchneri, whereas it was reduced (p<0.01) by added sugar. No significant difference was observed in count of yeast, but inoculation with L. buchneri shows a decreasing trend. Mould count in all silages was less than 2 (log cfu $g^{-1}$). The added sugar had negative effects on aerobic stability of maize silage made under air-stress conditions, whereas inoculation with L. buchneri improves (p<0.01) the aerobic stability.

키워드

참고문헌

  1. Driehuis, F., S. J. W. H. Oude Elferink and S. F. Spoelstra. 1999. Anaerobic lactic acid degradation during ensilage of whole crop maize inoculated with lactobacillus buchneri inhibits yeast growth and improves aerobic stability. Journal of Applied Microbiology 87(4):583-594. https://doi.org/10.1046/j.1365-2672.1999.00856.x
  2. Driehuis, F., S. F. Spoelstra, S. C. J. Cole and R. Morgan. 1996. Improving aerobic stability by inoculation with lactobacillus buchneri. In: Proceeding of the 11th international silage conference (Ed. D. I. H. Jone, R. R. Dewhurt, R. Merry and P. M. Haigh) IGER. Aberystwyth, pp. 106-107.
  3. Driehuis, F., S. J. W. H. Oude Elferink and S. F. Spoelstra. 1998. Inoculation of silage with a strain of lactobacillus buchneri inhibits yeast growth and improves aerobic stability. In: Lactic acid bacteria: Which strain? For which products? Proceeding lactic 97. Adria Normandie, Caen, France. pp. 549-550.
  4. Guan, W. T., G. Ashbell, Y. Hen and Z. G. Weinberg. 2002a. The Effects of Two Inoculants Applied to Forage Sorghum at Ensiling on Silage Characteristics. Asian-Aust. J. Anim. Sci. 15:218-221.
  5. Guan, W. T., F. Driehuis and P. G. van Wikselaar. 2002b. The influence of addition of sugar with or without L. buchneri on fermentation and aerobic stability of whole crop maize silage in anaerobic silos Asian-Aust J. Anim. Sci. 15:1128-1133.
  6. Kung, L., R. S. Tung and K. Maciorowski. 1991. Effects of a microbial inoculant ($Ecosyl^{TM}$) and/or a glycopeptide antibiotic (vancomycin) on fermentation and aerobic stability of wilted alfalfa silage. Anim. Feed Sci. Technol. pp. 35:37-48.
  7. Oude Elferink, S. J. W. H., F. Driehuis, J. C. Gottschal and S. F. Spoelstra. 1998. Regulation of the yeast population in maize silage by silage additive. In: Proceeding of the 8th international symposium on microbial ecology, Halifax, Canada. p. 259.
  8. Oude, Elferink, S. J. W. H. J. Krooneman, J. C. Gottschal, S. F. Spoelstra, F. Faber and F. Driehuis. 2001. Anaerobic conversion of lactic acid to acetic acid and 1, 2-propanediol by lactobacillus buchneri. Applied and Environmental Microbiology. 67:1-8. https://doi.org/10.1128/AEM.67.1.1-5.2001
  9. Reuter, G. 1985. Elective and selective media for lactic acid bacteria. J. Food. Microbiology 2:55-68.
  10. Sanderson, M. A. 1993. Aerobic stability and in vitro fiber digestibility of microbially inoculanted corn and sorghum silages. J. Anim. Sci. 71:505-514. https://doi.org/10.2527/1993.712505x
  11. SAS Institute Inc. 1989. SAS/STAT User’s Guide: Version 6. 4th edn. SAS Institute Inc.,Cary, North Carolina.
  12. Schmidt, J., J. Sipocz, I. Kaszas, G. Szakacs, A. Gyepes and R. P. Tengerdy. 1997. Preservation of sugar content in ensiled sweet sorghum. Bioresource Technology 60:9-13.
  13. Spoelstra, S. F. 1983. Inhibition of clostridia growth by nitrate during the early phase of silage fermentation. J. Sci. Food Agri. 34:145-152. https://doi.org/10.1002/jsfa.2740340206
  14. Van Vuuren, A. M., van D. E. R. Koelen, C. J. Valk and D. E. Visser. 1993. Effects of partial replacement of ryegrass by low protein feed on rumen fermentation and nitrogen loss by dairy cows. J. Dairy Sci. 76:2982-2993.
  15. Weinberg, Z. G. and R. E. Muck. 1996. New trends and opportunities in the development and use of inoculants for silages. FEMS Microbiology Reviews 19:53-68.
  16. Woolford, M. K. 1990. The detrimental effects of air in silage. Journal of Applied Bacteriology 68:101-116. https://doi.org/10.1111/j.1365-2672.1990.tb02554.x

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