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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Oxidative Modification of Neurofilament-L by Copper-catalyzed Reaction

  • Kim, Nam-Hoon (Department of Genetic Engineering, Chongju University) ;
  • Kang, Jung-Hoon (Department of Genetic Engineering, Chongju University)
  • Received : 2003.04.04
  • Accepted : 2003.04.23
  • Published : 2003.09.30
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Abstract

Neurofilament-L (NF-L) is a major element of neuronal cytoskeletons and known to be important for neuronal survival in vivo. Since oxidative stress might play a critical role in the pathogenesis of neurodegenerative diseases, we investigated the role of copper and peroxide in the modification of NF-L. When disassembled NF-L was incubated with copper ion and hydrogen peroxide, then the aggregation of protein was proportional to copper and hydrogen peroxide concentrations. Dityrosine crosslink formation was obtained in copper-mediated NF-L aggregates. The copper-mediated modification of NF-L was significantly inhibited by thiol antioxidants, N-acetylcysteine, glutathione, and thiourea. A thioflavin-T binding assay was performed to determine whether the copper/$H_2O_2$ system-induced in vitro aggregation of NF-L displays amyloid-like characteristics. The aggregate of NF-L displayed thioflavin T reactivity, which was reminiscent of amyloid. This study suggests that copper-mediated NF-L modification might be closely related to oxidative reactions which may play a critical role in neurodegenerative diseases.

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References

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