Journal of Gastric Cancer
- 제3권4호
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- Pages.206-213
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- 2003
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- 2093-582X(pISSN)
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- 2093-5641(eISSN)
위암 환자에 있어서 p53 유전자 돌연변이, 종양 p53 단백질 과발현 및 혈청 p53 항체
p53 Gene Mutation, Tumor p53 Protein Overexpression, and Serum Anti-p53 Antibody in Patients with Gastric Cancer
- Bong Jin-gu (Department of Surgery, Wallace Memorial Baptist Hospital) ;
- Lee Myung-Hoon (Departments of Pathology Kyungpook National University Hospital) ;
- Song Kyung-Eun (Departments of Laboratory Medicine, Kyungpook National University Hospital) ;
- Kim Taebong (Departments of Surgery, Kyungpook National University Hospital) ;
- Yu Wansik (Departments of Surgery, Kyungpook National University Hospital)
- 발행 : 2003.12.01
초록
Purpose: The clinical implication of p53 mutation in gastric cancer is still unclear, as shown by the discordant results that continue to be reported in the literature. Materials and Methods: To assess p53 gene mutation, tumor p53 overexpression, and serum anti-p53 antibody, we employed a polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis, an immunohistochemistry using monoclonal antibody DO-7, and an enzymelinked immunosorbent assay (ELISA), respectively. Results: Of 169 surgical specimens of gastric cancer, mutation at exon