The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Screening of Mushrooms Having Angiotensin I-Converting Enzyme Inhibitor

각종 버섯류로부터 안지오텐신 전환효소 저해제의 탐색

  • Lee, Dae-Hyoung (Department of Genetic Engineering and Bio-medicinal Resource Research Center, Paichai University) ;
  • Kim, Jae-Ho (Department of Genetic Engineering and Bio-medicinal Resource Research Center, Paichai University) ;
  • Cheong, Jong-Chun (National Institute of Agricultural Science and Technology) ;
  • Gong, Won-Shik (National Institute of Agricultural Science and Technology) ;
  • Yoo, Young-Bok (National Institute of Agricultural Science and Technology) ;
  • Park, Jeong-Sik (National Institute of Agricultural Science and Technology) ;
  • Yoo, Chang-Hyun (National Institute of Agricultural Science and Technology) ;
  • Lee, Jong-Soo (Department of Genetic Engineering and Bio-medicinal Resource Research Center, Paichai University)
  • 이대형 (배재대학교 유전공학과.바이오의약연구센타) ;
  • 김재호 (배재대학교 유전공학과.바이오의약연구센타) ;
  • 정종천 (농업과학기술원) ;
  • 공원식 (농업과학기술원) ;
  • 유영복 (농업과학기술원) ;
  • 박정식 (농업과학기술원) ;
  • 유창현 (농업과학기술원) ;
  • 이종수 (배재대학교 유전공학과.바이오의약연구센타)
  • Published : 2003.12.30
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Abstract

Extracts from 52 samples of mushrooms were prepared by using water, ethanol and methanol, and then yields and angiotensin I-converting enzyme(ACE) inhibitory activity were investigated. Sample mushrooms contained crude proteins of $7.1{\sim}56.5%$, curde lipids of $0.2{\sim}4.4%$ and carbohydrates of $30.3{\sim}86.6%$. Among 52 samples, the water extract from fruiting body of Pholiota spp. ASI 24027 showed the highest extraction yield of 68%. Water extract of Pholiota spp. ASI 24012 fruiting body had potential ACE inhibitory activity of 66%. The optimal extraction condition of the ACE inhibitor from the fruiting bodyies of Pholiota spp. ASI 24012 was In water at $30^{\circ}C$ for 1 hr and ACE inhibitory activity was 67.6% on the condition with 0.2 mg of $IC_{50}$.

항고혈압 효능이 우수하며 부작용이 없는 고혈압 예방 제품을 개발하고자 농업과학기술원에서 분양 받은 52종의 버섯을 대상으로 일반성분, 추출 수율, 그리고 ACE 저해활성을 조사하여 활성이 우수한 버섯을 선정한 다음 ACE 저해물질의 추출 최적조건을 검토하였다. 시료 버섯은 $7.1{\sim}56.5%$의 조단백질과 $0.2{\sim}4.4%$의 조지방 및 $30.3{\sim}86.6%$의 탄수화물을 각각 함유하고 있었으며 비늘버섯 ASI 24027 자실체의 물 추출물 수율이 68%로 제일 높았다. 그러나 ACE 저해활성은 비늘 버섯 ASI 24012 균주의 자실체를 물 추출로부터 얻은 추출물($IC_{50}$: 0.45 mg)에서 가장 높았다. 그리고 이 버섯의 ACE 저해물질 최적추출조건은 자실체 분말을 물로 $30^{\circ}C$에서 1시간 추출했을때 가장 많이 용출 되었으며 이때 ACE 저해활성도도 67.6%($IC_{50}$: 0.20 mg)로 가장 높았다.

Keywords

References

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