한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
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온실재배와 노지재배한 금산 깻잎의 생리기능성

Physiological Functionality in Geumsan Perilla Leaves from Greenhouse and Field Cultivation

  • 발행 : 2003.10.01
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초록

깻잎 중의 각종 성인병 관련 생리기능성을 탐색하여 고부가가치의 건강식품을 개발하고자 먼저 깻잎의 주산지중의 한곳인 금산에서 1월, 5월 온실재배한 깻잎들과 8월 노지재배한 깻잎의 추출물 수율을 조사하였다. 8월 노지에서 재배한 깻잎의 에탄올 추출물 수율이 29%로 제일 높았고, 1월과 5월 온실재배 깻잎보다는 8월 노지재배 깻잎의 추출물 수율이 더 높았다. 물과 30% 에탄올 추출물에 대한 생리기능으로 혈전용해활성은 5월 온실재배 깻잎의 에탄올 추출물이 8.2U로 제일 높았고 고지혈증 예방에 관련된 HMG-CoA reductase 저해활성은 8월 노지재배의 물 추출물에서 83%의 높은 활성을 보였다. 항고혈압활성을 나타내는 안지오텐신 전환효소(ACE) 저해활성은 1월 온실재배 깻잎의 물 추출물에서 64.5%의 높은 활성을 보였다. 항산화활성은 물 추출물 보다는 대체로 30% 에탄올 추출물에서 높았고, 특히 8월 노지재배 깻잎의 에탄올 추출물에서 69%를 보였다. Elastase 저해활성은 5월 온실재배 깻잎의 30% 에탄올 추출물에서 47.5%의 활성을 보였고 혈전용해활성과 아질산염 제거활성, SOD-유사활성과 tyrosinase 저해활성 등은 모든 깻잎 추출물에서 없거나 매우 미약하였다.

Perilla leaves cultivated in greenhouses (Jan., May) and in the fields (Aug.) of Geumsan province were investigated for their extract yields and physiological functionalities. The yield was highest in 30% ethanol extracts of the August perilla leaves. The highest fibrinolytic activity (8.2 U) was observed in 30% ethanol extracts of the May perilla leaves, while the HMG-CoA reductase inhibition level, which is related to the inhibition of cholesterol biosynthesis, was 83% in water extracts of the August perilla leaves. Anti-hypertensive ACE inhibitory activity was 64.5% in the water extracts of the January perilla leaves, and antioxidative electron donating ability was the highest (69%) in 30% ethanol extracts of the August perilla leaves. Elastase inhibitory activity, which is related to the inhibition of skin aging, was highest (47.5%) in 30% ethanol extracts of the May perilla leaves. However, SOD-like activity, nitrite scavenging activity, and tyrosinase inhibitory activity were not detected were very weak in all samples.

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참고문헌

  1. Kim, J.H. and Kim, M.K. Effect of dried leaf powders and ethanol extracts of Perilla frutescens, artemisia prineceps var. orientalis and aster scaber on lipid metabolism and antioxidative capacity in rats. J. Korean Soc. Nutr. 32: 540-551(1999)
  2. Lee, K.I, Rhee, S.H., Kim, J.O., Chung, H.Y. and Park, K.Y. Antimutagenic and antioxidative effects of perilla leaf extracts. Korean J. Soc. Food Nutr. 22: 175-180 (1993)
  3. Kim, T.J. Natural Plant Resources in Korea. Seoul National University Press, Seoul, Korea (1996)
  4. Ohaba, T., Haseo, T., Akita, O. and Yamamoto, Y. A liqueur using the extract of Perilla ocimoides var. Crispa. J. Brewing Soc. Japan 80: 278-281 (1985) https://doi.org/10.6013/jbrewsocjapan1915.80.278
  5. Kim, K.H., Chang, M.W., Park, K.Y, Rhee, S.H., Rhew, T.H. and Sunwoo, Y. Antitumor activity of phytol identified from perilla leaf and its augmentative effect on cellular immune response. Korean J. Food Nutr. 26: 379-389 (1993)
  6. Lee, K.I., Rhee, S.H. and Park, K.Y. Anticancer activity of phytol and eicosatrienoic acid identified from perilla leaves. Korean J. Soc. Food Sci. Nutr. 28: 1107-1112 (1999)
  7. Park, K.Y., Lee, K.I. and Lee, S.H. Antimutagenic effects and inhibitory effect in development of AZ-521 gastric tumor cell in green and yellow vegetables. J. Korean Soc. Food Nutr. 21: 149-153 (1992)
  8. Lee, K.I., Rhee, S.K., Park, K.Y. and Kim, J.O. Antimutagenic compounds identified from perilla leaf. Korean J. Soc. Food Nutr. 21: 302-307 (1992)
  9. Toyoda, M., Tanak, K., Hoshimo, K., Akiyama, H., Tanimura, A. and Saito, Y. Profiles of potentially antiallergic flavonoids in 27 kinds of health tea and green tea infusions. J. Agric. Food Chem. 45: 2516-2564 (1997)
  10. Tada, M., Matsumoto, R., Yamaguchi, H. and Chiba, K. Novel antioxidants isolated from Perilla frutescens Britton var. crisp (Thunb.). Biosci. Biotech. Biochem. 60: 109 3-1095 (1996) https://doi.org/10.1271/bbb.60.1093
  11. Hong, Y.P., Kim, S.Y. and Choi, W.Y. Postharvest changes in quality and biochemical components of perilla leaves. Korean J. Food Sci. Technol. 18: 255-258 (1986)
  12. Fayek, K.I. and El-Sayed, S.T. Purification and properties of fibrinolytic enzyme from Bacillus subtilis. Zeit. fur Allgem. Mikrobiol. 20: 375-382 (1980) https://doi.org/10.1002/jobm.3630200603
  13. Ha, T.Y., Cho, I.J. and Lee, S.H. Screening of HMG-CoA reductase inhibitory activity of ethanol and methanol extracts from cereals and regumes. Korean J. Food Sci. Technol. 30: 224-229 (1998)
  14. Cushman, D.W. and Cheung, H.S. Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem. Pharmacol. 20: 1637-1648 (1971) https://doi.org/10.1016/0006-2952(71)90292-9
  15. Blois, M.S. Antioxidant determination by the use of stable free radical. Nature 191: 1199-1201 (1958) https://doi.org/10.1038/1911199a0
  16. Marklund, S. and Marklund, G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. J. Eur. Biochem. 47: 469-474 (1974) https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  17. Cho, J.J. Isolation and characterization of elastase inhibitor from Areca catechu. M.S. thesis, Chungbuk National Univ., Chungbuk, Korea (1999)
  18. Sun, C.K. and Cho, S.H. Studies on the pufification and characteristics of tyrosinase from Diospyros kaki Thunb. Korean J. Biochem. 25: 79-87 (1992)
  19. Kato, H., Lee, I.H., Chuyen, N.V, Kim, S.B. and Hayase, F. Inhibition of nitrosamine formation by nondialyzable melanoidins. Agric. Biol. Chem. 51: 1333-1338 (1987) https://doi.org/10.1271/bbb1961.51.1333
  20. National Rural Living Science Institute. Food Composition Table. 6th Revision. Sangrogsa, Suweon, Korea (2001)
  21. Hwang, I.J. Emaehrungsphysiologische wirkungen laktofermentierter kohlgemuese: Einfluss regelmaessiger Aufnahme von Sauerkraut und Kimchi auf verschie den Blutparameter. Ph.D. dissertation, Diss. Univ., Giessen, Germany (1991)
  22. Jeong, Y.K., Yang, E.S., Kang, J.W., Gong, I.S. and Kim, J.O. Fibrinolysis of fermented kimchi. Korean J. Life Sci. 5: 203-210 (1995)
  23. Srivastava, K.C., Brodia, A. and Verma, S.K. Garlic (AIIium sativum) for disease prevention. South Afiican J. Sci. 91: 68-72 (1995)
  24. Kim, M.J., Song, Y.S. and Song, Y.O. The fibrinolytic activity of Kimchi and its ingredients in vivo and in vitro. Korean J. Soc. Food Sci. Nutr. 27: 633- 638 (1998)
  25. Bae, I.Y. Studies of the inhibitory activity of angiotensin I-converting enzyme of various vegetables. M.S. thesis, Hanyang Univ., Seoul, Korea (1996)
  26. Rhyu, M.R., Nam, R.J. and Lee, H.Y. Screening of angiotensin I-converting enzyme inhibitors in cereals and legumes. J. Biotechnol. 4: 334-337 (1996)
  27. Lee, D.H., Kim, J.H., Kim, N.M. and Lee, J.S. Manufacture and physiological functionality of Korea traditional liquors by using chamomile (Matricariachamomile). Korean J. Food Sci. Technol. 34: 109-113 (2002)