Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Effects of Wolguk-whan Water Extract on Acute Oxidative Liver Injury Induced by Acetaminophen

월국환(越鞠丸) 물 추출물이 Acetaminophen으로 유도된 마우스의 급성 간손상에 미치는 효과

  • Lee Chae-Jung (Dept. of Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Park Sun-Dong (Dept. of Oriental Medical Prescription, College of Oriental Medicine, Dongguk University) ;
  • Moon Jin-Young (Dept. of AM-Pointology, College of Oriental Medicine, Dongguk University)
  • 이채중 (동국대학교 한의과대학 내과학교실) ;
  • 박선동 (동국대학교 한의과대학 방제학교실) ;
  • 문진영 (동국대학교 한의과대학 경혈학교실)
  • Published : 2003.12.30
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Abstract

Objectives : Wolguk-whan has been used as a prescription of natural drug for the treatment of stress digestive system disease. Recently, we reported that Wolguk-whan methnol extract (WGWM) exerted a significant protective effect against oxidative damage to the liver of ICR mice. This study was purposed to investigate the effects of Wolguk-whan water extract (WGWW) on liver injury induced by oxidative stress. Methods : In order to investigate the effects of WGWW on acute liver injury, ICR mice were pretreated with WGWW for 6days, starved for 24hrs, and administerated acetamirtophen(500mg/kg, i.p.). In the liver homogenates, lipid peroxide and glutathione(GSH) levels were measured. In addition, activities of hepatic enzyme, such as catalase, glutathione peroxidase(GSH-Px), glutathione S-transferase(GST) were measured in the hepatic mitochondrial and cytosolic fractions. Results : In vivo administeration of WGWW showed effective inhibition of acetaminophen induced lipid peroxidation, and showed elevations of GSH level, catalase, GSH-Px, GST activities. Conclusions : These results suggested that WGWW might suppress the formation of oxidative metabolites, and prevent acetaminophen induced hepatotoxicity.

Keywords

References

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